Profiling of miRNAs in porcine germ cells during spermatogenesis

Chen, Xiaoxu; Che, Dongxue; Zhang, Pengfei; Li, Xueliang; Yuan, Quan; Liu, Tiantian; Guo, Jiayin; Feng, Tongying; Wu, Ligang; Liao, Minzhi; He, Zuping; Zeng, Wenxian

doi:10.1530/rep-17-0441

Cited by 33 publications

(24 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These observations led us to hypothesize that boar SP-EVs may participate in porcine spermatogenesis. Ssc-miR-10a-5p is highly expressed in spermatogonia (43) and is also abundant in the SP-EVs, suggesting that boar SP-EVs may partially derive from testis and influence spermatogenesis. The miRNA ssc-miR-200b has been shown to target porcine spermatogenesis-associated serine-rich 2-like (SPATS2L) gene, which significantly affects litter size (44), suggesting that boar SP-EVs may affect sperm function and influence embryo development.…”

Section: Discussionmentioning

confidence: 99%

Expression Pattern of Seminal Plasma Extracellular Vesicle Small RNAs in Boar Semen

Xie

Zhou

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

Extracellular vesicles (EVs) regulate multiple physiological processes. Seminal plasma contains numerous EVs that may deliver functional molecules such as small RNAs (sRNAs) to the sperm. However, the RNA profiles in the boar seminal plasma extracellular vesicles (SP-EVs) and its function have not been characterized. The aim of this study was to characterize the functions and sRNA profiles in the boar SP-EVs using deep sequencing technology. Briefly, boar SP-EVs were isolated by differential ultracentrifugation and confirmed with a transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and Western blot. The isolated boar SP-EVs contained numerous and diverse sRNA families, including microRNAs (miRNAs, 9.45% of the total reads), PIWI-interacting RNAs (piRNAs, 15.25% of the total reads), messenger RNA fragments (mRNA, 25.30% of the total reads), and tRNA-derived small RNAs (tsRNA, 0.01% of the total reads). A total of 288 known miRNAs, 37 novel miRNA, and 19,749 piRNAs were identified in boar SP-EVs. The identified ssc-miR-21-5p may confer negative effects on sperm fertility based on a dual-luciferase reporter experiment. This study therefore provides an effective method to isolate SP-EVs and characterizes the sRNA profile.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression Pattern of Seminal Plasma Extracellular Vesicle Small RNAs in Boar Semen

Xie

Zhou

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Total RNA was extracted using RNAiso Plus reagent (TaKaRa, Dalian, China). RT-qPCR was performed as described previously using the primers listed in Supplementary Table S1 ( Chen et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

Chen

Liu

et al. 2020

Front. Genet.

Self Cite

View full text Add to dashboard Cite

SETDB1, a histone H3 lysine 9 (H3K9) methyltransferase, is crucial in meiosis and embryo development. This study aimed to investigate whether SETDB1 was associated with spermatogonial stem cells (SSC) homeostasis. We found that knockdown of Setdb1 impaired cell proliferation, led to an increase in reactive oxygen species (ROS) level through NADPH oxidase, and Setdb1 deficiency activated ROS downstream signaling pathways, including JNK and p38 MAPK, which possibly contributed to SSC apoptosis. Melatonin scavenged ROS and rescued the phenotype of Setdb1 KD. In addition, we demonstrated that SETDB1 regulated NADPH oxidase 4 ( Nox4 ) and E2F1 . Therefore, this study uncovers the new roles of SETDB1 in mediating intracellular ROS homeostasis for the survival of SSC.

show abstract

“…Small RNA-Seq and analysis were performed as previous report [26]. Briefly, total RNA was used for adaptor ligation, first strand cDNA synthesis and PCR amplification to construct small RNA libraries.…”

Section: Small Rna-seq and Analysismentioning

confidence: 99%

“…To further analyze the expression pattern of miRNAs in SCs, we compared SC seq dada with small RNA data from germ cells (GSE124099) that was reported previously [26]. Principal component analysis (PCA) revealed that three SC samples clustered together ( Fig.…”

Section: Analysis Of Mirna Expression In Scs and Germ Cellsmentioning

confidence: 99%

Profiling of miRNAs in porcine Sertoli cells

Chen

Zheng

et al. 2020

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

Background: Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), a kind of~22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown. Methods: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor (TNFR)-associated factor 3 (TRAF3) by ssc-miR-149. Results: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3. Conclusion: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.

show abstract

Profiling of miRNAs in porcine germ cells during spermatogenesis

Cited by 33 publications

References 57 publications

Expression Pattern of Seminal Plasma Extracellular Vesicle Small RNAs in Boar Semen

Expression Pattern of Seminal Plasma Extracellular Vesicle Small RNAs in Boar Semen

The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

Profiling of miRNAs in porcine Sertoli cells

Contact Info

Product

Resources

About