Prognostic Impact of Hypoxia-Inducible<i>miRNA-210</i>in Patients with Lung Adenocarcinoma

Osugi, Jun; Kimura, Yoshihide; Owada, Yohei; Inoue, Takuya; Watanabe, Yuzuru; Yamaura, Takumi; Fukuhara, Mitsuro; Muto, Satoshi; Okabe, Naoyuki; Matsumura, Yuki; Hasegawa, T.; Yonechi, Athushi; Hoshino, Mika; Higuchi, Mitsunori; Shio, Yutaka; Suzuki, Hiroyuki; Gotoh, Mitsukazu

doi:10.1155/2015/316745

Cited by 34 publications

(29 citation statements)

References 47 publications

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“…This result was consistent with previous findings in which miR-210 levels were increased in lung cancer patients (34)(35)(36)(37). MiR-210 levels expression also was a prognostic factor for survival of lung cancer patients (38). However, the mechanism of miR-210 upregulation has not been completely clear.…”

Section: Discussionsupporting

confidence: 92%

Role of plasma MicroRNAs in the early diagnosis of non-small-cell lung cancers: a case-control study

et al. 2016

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Section: Discussionsupporting

confidence: 92%

Role of plasma MicroRNAs in the early diagnosis of non-small-cell lung cancers: a case-control study

et al. 2016

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“…There were a total of 2 GEO datasets and 4 public studies [35, 41-43], as well as our own RT-qPCR results with an overall number of 396 patients included in this meta-analysis (Table 5). A fixed model was selected for estimation as no significantly high heterogeneity was observed.…”

Section: Resultsmentioning

confidence: 99%

Clinical Significance of miR-210 and its Prospective Signaling Pathways in Non-Small Cell Lung Cancer: Evidence from Gene Expression Omnibus and the Cancer Genome Atlas Data Mining with 2763 Samples and Validation via Real-Time Quantitative PCR

Cen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Since the function of microRNA (miR)-210 in non-small cell lung cancer (NSCLC) remains unclear, we aimed to explore the clinical significance of miR-210 in NSCLC. Methods: NSCLC-related data from 1673 samples on Gene Expression Omnibus and 1090 samples on The Cancer Genome Atlas were obtained and analyzed. The expression level of miR-210 was validated via real-time quantitative PCR analysis with 125 paired clinical samples. A meta-analysis was performed to generate a comprehensive understanding of miR-210 expression and its clinical significance in NSCLC. In addition, bioinformatics analysis was also conducted to reveal the potential underlying mechanism of miR-210 action in NSCLC. Results: miR-210 expression was consistently elevated in NSCLC solid tissue samples. However, its expression was controversial in easily obtained body fluids (i.e., blood, plasma, and serum). Moreover, an overall pooled meta-analysis implied a comparatively higher level of miR-210 expression in NSCLC cancerous tissue than in normal control tissue (P < 0.001). In addition, a meta-analysis of outcome revealed a significant diagnostic capacity of miR-210 in NSCLC by detecting its expression in serum and sputum (area under the summary receiver operating characteristic curve 0.82 and 0.81, respectively). miR-210 overexpression was associated with poor progression-free survival (PFS) in NSCLC and was negatively related to overall survival and disease-free survival. Bioinformatic gene enrichment and annotation analyses showed that the target genes of miR-210 were greatly enriched in cell adhesion and plasma membrane, and three pathways were considered to be the main functional circuits of miR-210: renin secretion, the cGMP-PKG signaling pathway, and cell adhesion molecules. Conclusion: In NSCLC, miR-210 expression was elevated and overexpression indicated poor PFS. Expression level of miR-210 in serum and sputum showed significant diagnostic value for NSCLC.

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“…VEGF can contribute to tumorigenesis, by promoting angiogenesis and enhancing the blood supply (35). Moreover, miR-210 has been reported to be the most highly induced miRNA in hypoxic cells (36), and hypoxia reduces the radiosensitivity of tumor cells both in vitro and in vivo (37). Previous studies have reported that some miRNAs can regulate the DNA damage response to radiation and participate in DNA repair pathways (38,39), but the mechanisms involved in radioresistance, angiogenesis, apoptosis, cell cycle control and DNA damage/repair are still unknown.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells

Luo

Zhao

et al. 2017

Molecular Medicine Reports

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Radiotherapy is one of the primary methods of treatment of malignant tumors, however, resistance to radiation is a major problem. The reasons for the radioresistance are still poorly understood. However, it is generally accepted that microRNAs (miRNAs or miRs) can regulate the radiosensitivity of tumors. The present study therefore aimed to identify specific miRNAs and their effects on radioresistant cells. More specifically, the aim was to investigate specific miRNAs and their effects on radioresistant tumor cells. The radioresistant tumor cells (CNE‑2R) were established using a dose gradient method, and the miRNA expression profiles of CNE‑2R cells and the parental cells (CNE‑2) were determined. The expression of miR‑210 in CNE‑2R cells was significantly higher than in CNE‑2 cells. CNE‑2R cells were transfected with LV‑hsa‑miR‑210‑inhibitor, and CNE‑2 cells were transfected with LV‑hsa‑miR‑210. The expression of miR‑210 was confirmed by reverse transcription quantitative‑polymerase chain reaction. The percentages of CNE‑2R‑miR‑210‑inhibitor and CNE‑2 cells in the G2/M phase were higher than in the CNE‑2R and CNE‑2‑miR‑210 cells, and the percentages of cells in S phase were lower than in the CNE‑2R and CNE‑2‑miR‑210 cells. Following 4 Gy of radiation, CNE‑2R‑miR‑210‑inhibitor and CNE‑2 cells, which express low levels of miR‑210, had a higher apoptosis rate than CNE‑2R and CNE‑2‑miR‑210 cells. Following 4, 8 and 12 Gy of radiation, cell viability and survival fraction of CNE‑2R‑miR‑210‑inhibitor cells were lower than those of CNE‑2R and CNE‑2‑miR‑210 cells, and similar to those of CNE‑2 cells. Together, these findings strongly suggest that miR‑210 negatively regulates the radiosensitivity of tumor cells, and may therefore have therapeutic potential for the treatment of radiation resistance.

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Prognostic Impact of Hypoxia-InduciblemiRNA-210in Patients with Lung Adenocarcinoma

Cited by 34 publications

References 47 publications

Role of plasma MicroRNAs in the early diagnosis of non-small-cell lung cancers: a case-control study

Role of plasma MicroRNAs in the early diagnosis of non-small-cell lung cancers: a case-control study

Clinical Significance of miR-210 and its Prospective Signaling Pathways in Non-Small Cell Lung Cancer: Evidence from Gene Expression Omnibus and the Cancer Genome Atlas Data Mining with 2763 Samples and Validation via Real-Time Quantitative PCR

MicroRNA-210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells

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