Prognostic significance of TBL1XR1 in predicting liver metastasis for early stage colorectal cancer

Liu, Hongda; Xu, Yaping; Zhang, Qun; Yang, Hui; Shi, Weichen; Liu, Zhaochen; Li, Kangshuai; Gong, Zheng; Ning, Shanglei; Li, Shuo; Chen, Yuxin

doi:10.1016/j.suronc.2016.12.003

Cited by 75 publications

(63 citation statements)

References 18 publications

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“…Total RNA of HCC tissues and normal liver tissues was extracted with Trizol reagent according to the manual 14. The cDNA synthesis and quantitative PCR were performed by StepOnePlus real-time PCR system (Thermo Fisher Scientific) and SYBR Green method.…”

Section: Methodsmentioning

confidence: 99%

Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Hu¹,

Wang²,

Wang³

et al. 2017

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AimTo investigate the expression and clinical significance of Holliday junction–recognizing protein (HJURP) in hepatocellular carcinoma (HCC).MethodsIn this study, we detected the expression of HJURP protein in samples of 164 patients with HCC, and based on this, we divided the patients into two cohorts: high expression of HJURP and low expression of HJURP. We analyzed the correlation between HJURP expression and the clinicopathological factors using chi-square test. Survival significance of HJURP was defined by Kaplan–Meier method and log-rank test, and the independent prognostic factors were identified by Cox regression model. Using function assays of HCC cell lines, we investigated the influence of HJURP on the proliferation of HCC cells.ResultsIn our study, the proportion of patients with high HJURP expression was 25.6%, which was significantly associated with the tumor size and Barcelona clinic liver cancer stage. Univariate analysis confirmed that high HJURP expression was remarkably associated with poorer overall survival rates (P=0.003), as well as tumor number (P=0.016), tumor differentiation (P=0.047), TNM stage (P=0.005), and Barcelona clinic liver cancer stage (P=0.004). Multivariate analysis confirmed that high HJURP expression (P<0.001) acted as an independent prognostic risk factor of unfavorable prognosis. Real-time polymerase chain reaction analysis revealed that the expression of HJURP was significantly higher in HCC tissues than that in the corresponding normal liver tissues. Moreover, we demonstrated that HJURP overexpression could accelerate HCC cell line proliferation, whereas HJURP knockdown could attenuate the proliferation.ConclusionHigh HJURP expression was an independent prognostic biomarker of HCC, predicting poorer prognosis. HJURP also played an important role in HCC cell proliferation.

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Section: Methodsmentioning

confidence: 99%

Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Hu¹,

Wang²,

Wang³

et al. 2017

OTT

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“…The cutoff of IHC score divided the cohort into low SMYD3 expression group and high SMYD3 expression group. The cut-off was determined by the receiver operating characteristic (ROC) curve and defined as the point with the highest sum of the specificity plus sensitivity according to previous report (Liu et al 2017b). In our study, the calculated cut-off was 5.4, which means cases with IHC score ≥ 6 was set as high SMYD3 expression group.…”

Section: Immunohistochemical Evaluationmentioning

confidence: 99%

Overexpression of SMYD3 Is Predictive of Unfavorable Prognosis in Hepatocellular Carcinoma

Xiao

Liu

et al. 2017

Tohoku J. Exp. Med.

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SET and MYND domain-containing protein 3 (SMYD3) is a kind of histone lysine methyltransferase, responsible for transcriptional activation as a member of an RNA polymerase complex. The ectopic expression of SMYD3 is proved to promote the progress of many kinds of cancers. In hepatocellular carcinoma (HCC), SMYD3 was demonstrated to promote the proliferation and metastasis of HCC cell lines, but the clinical significance of SMYD3 has not been elucidated. In the present study, we detected the expression of SMYD3 in 100 HCC tissues with immunohistochemistry and divided these tissue specimens into high-expression group and low-expression group according to the immunohistochemical score of SMYD3. Importantly, the intensity of SMYD3 immunoreactivity was significantly stronger in HCC tissues than that in adjacent normal tissues. Moreover, high expression levels of SMYD3 were significantly associated with larger tumor size (P = 0.043), suggesting that SMYD3 could promote the proliferation of HCC. Moreover, patients with positive hepatitis B virus infection had higher expression levels of SMYD3 (P = 0.013). With univariate and multivariate analysis, we explored the prognostic significance of SMYD3 in HCC. As a result, high expression levels of SMYD3 were significantly correlated to the poorer clinical outcome of HCC patients (P = 0.009) and were identified as an independent risk factor of HCC for predicting the unfavorable prognosis. In conclusion, overexpression of SMYD3 is an independent prognostic risk of unfavorable prognosis of HCC. We propose that the anti-SMYD3 therapy may be a potential approach to treat HCC.

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“…In prostate cancer, TBL1XR1 was reported to function as a tumor suppressor and inhibit tumor growth, perhaps through regulating androgen receptor (AR) signaling pathway (Daniels et al 2014. However, TBL1XR1 has been reported to be highly expressed in cervical cancer , breast cancer , nasopharyngeal carcinoma , hepatocellular carcinoma (Kuang et al 2016), digestive cancers (Liu et al 2016a;Zhou et al 2016;Liu et al 2017), and ovarian cancer . However, the significance of TBL1XR1 in predicting clinical outcomes of EOC patients has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer

2017

Tohoku J. Exp. Med.

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Transducin (β)-like 1 X-linked receptor 1 (TBL1XR1) is a core component of the NCoR/SMRT transcription co-repressor complex, and its role in regulating cancer progression has been reported. Serous epithelial ovarian cancer (EOC) is the most common histological type of EOC. Here we explored the significance of TBL1XR1 expression in predicting outcomes of patients with serous EOC. Real-time quantitative PCR analysis showed that the expression level of TBL1XR1 mRNA was significantly higher in EOC tissues compared with adjacent non-tumorous tissues. The protein levels of TBL1XR1 in EOC tissues were assessed by immunohistochemistry, and the patients were classified into low-expression group (n = 62) and high-expression group (n = 54) according to the immunoreactivity. Prognostic significance of TBL1XR1 was evaluated by univariate and multivariate analyses, showing that over-expression of TBL1XR1 was correlated with poor prognosis. In addition, TBL1XR1 was positively associated with the lymph node metastasis of EOC. Because vascular endothelial growth factor (VEGF)-C is known as a critical mediator of lymph node metastasis, we measured the expression level of VEGF-C mRNA in EOC tissues and thus identified a positive correlation between TBL1XR1 and VEGF-C mRNA levels. Subsequently, using human EOC cell lines, we showed that silencing of TBL1XR1 decreased VEGF-C expression, suggesting that TBL1XR1 may function as an upstream regulator of VEGF-C in EOC. Furthermore, the proliferation and invasion of EOC cells were inhibited by TBL1XR1 silencing. In conclusion, TBL1XR1 overexpression may be an unfavorable prognostic factor for EOC. We also suggest that the TBL1XR1-VEGF-C axis may determine the EOC progression.

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Prognostic significance of TBL1XR1 in predicting liver metastasis for early stage colorectal cancer

Cited by 75 publications

References 18 publications

Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Overexpression of SMYD3 Is Predictive of Unfavorable Prognosis in Hepatocellular Carcinoma

Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer

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Prognostic significance of TBL1XR1 in predicting liver metastasis for early stage colorectal cancer

Cited by 75 publications

References 18 publications

Holliday junction&ndash;recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Holliday junction&ndash;recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Overexpression of SMYD3 Is Predictive of Unfavorable Prognosis in Hepatocellular Carcinoma

Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer

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Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma