Prognostic Value of Expression of MicroRNAs in Non-Small Cell Lung Cancer: A Systematic Review and Meta-Analysis

Zhan, Bingxiang; Lu, Dapeng; Luo, Peng; Wang, Baolong

doi:10.7754/clin.lab.2016.160426

Cited by 35 publications

(31 citation statements)

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“…Studies have shown that miR-125b, as an oncogene, is highly expressed in lung cancer cells/tissues/serums and is associated with poor outcome of NSCLC patients. [29][30][31][32][33] Our study indicated that the G allele of rs512932 might play a role in the development and prognosis of non-small-cell lung cancer through affecting the binding of the transcription factor, which then alters miR-125b expression. Rs2241490 was not associated with the transcriptional activity of miR-125b-1.…”

Section: F I G U R E 1 Kaplan-meier Plots Of Survival According Tomentioning

confidence: 81%

Genetic variations in miR‐125 family and the survival of non‐small cell lung cancer in Chinese population

Shen

Yang

et al. 2019

Cancer Medicine

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To investigate the associations between the functional single nucleotide polymorphisms (SNPs) in the miR‐125 family and the survival of non‐small cell lung cancer (NSCLC) patients, we systematically selected six functional SNPs located in three pre‐miRNAs (miR‐125a, miR‐125b‐1, miR‐125b‐2). Cox proportional hazard regression analyses were conducted to estimate the crude and adjusted hazard ratios (HRs) and their 95% confidence intervals (CIs). Reporter gene luciferase assay was performed to examine the relationship between the SNPs and transcriptive activity of the miRNAs. The expression of miRNAs in different cells was detected using quantitative real‐time PCR assay. We found that rs2241490 (upstream of miR‐125b‐1, G > A, adjusted HR = 1.24, 95%CI = 1.05‐1.48, P = 0.014, in dominant model; adjusted HR = 1.18, 95%CI = 1.03‐1.35, P = 0.014, in additive model), rs512932 (upstream of miR‐125b‐1, A > G, dominant model: adjusted HR = 1.25, 95%CI = 1.05‐1.48, P = 0.013) and rs8111742 (upstream of miR‐125a, G > A, dominant model: adjusted HR = 0.84, 95%CI = 0.71‐1.00, P = 0.047) were associated with the prognosis of 1001 Chinese NSCLC patients. The combined analysis of the three SNPs related the number of risk alleles (rs2241490‐A, rs512932‐G and rs8111742‐G) to death risk of NSCLC in a locus‐dosage mode ( P for trend <0.001). Furthermore, luciferase reporter gene assay showed significantly higher levels of luciferase activity with rs512932 variant G than that with A allele in 293T, SPC‐A1 and A549 cell lines. Besides, miR‐125b was highly expressed in lung cancer cells than the normal lung cell. Our study indicated that genetic variations in miR‐125 family were implicated in the survival of NSCLC patients. Larger population‐based and functional studies are needed to verify these findings.

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Section: F I G U R E 1 Kaplan-meier Plots Of Survival According Tomentioning

confidence: 81%

Genetic variations in miR‐125 family and the survival of non‐small cell lung cancer in Chinese population

Shen

Yang

et al. 2019

Cancer Medicine

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“…Epidemiologic studies and meta-analysis have shown that prolonged use of NSAIDs reduces the risk of several solid tumor including lung cancer, and recent meta-analysis suggests that low-dose aspirin could reduce the relative risk of cancer mortality (Zhan et al 2013;Vaish and Sanyal 2011;Setia and Sanyal 2012). Both clinical and experimental studies support the anti-neoplastic effects of NSAIDs mediated by regulation of COX-2 levels and induction of apoptosis (Haynes et al 2003).…”

Section: Inhibitors Of Inflammation For the Prevention And Treatment mentioning

confidence: 87%

The Role of Inflammation in Lung Cancer

Gomes

Teixeira

Coelho

et al. 2014

Advances in Experimental Medicine and Biology

212

165

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Lung cancer remains a serious public health problem and is the first cause of cancer death worldwide, and the overall 5-year survival rate for all stages is 14-17 % for Non-small-cell lung cancer and 6 % for small-cell lung cancer. Clinical and epidemiologic studies have suggested a strong association among chronic infection, inflammation, and cancer. Immune system plays a critical role in maintaining tissue homeostasis, cell turnover, tissue remodeling, and preventing infection and cell transformation. The inflammatory component in the development of the neoplasm includes a diverse leukocyte population; these components are considered inflammatory tumor key factors promoting tumor progression due to its ability to release a variety of cytokines, chemokines, and cytotoxic mediators such as reactive oxygen species (ROS), metalloproteinases, interleukins, and interferons. Cancer-related inflammation affects many aspects of malignancy, including the proliferation and survival of malignant cells, angiogenesis, tumor metastasis, and tumor response to chemotherapeutic drugs and hormones. Moreover, epidemiologic studies and meta-analysis have shown that prolonged use of non-steroid anti-inflammatory (NSAID) drugs reduces the risk of several solid tumor including lung cancer. Strong lines of evidence suggest that the chemopreventive properties of chronic NSAID administration are based on their COX-inhibitory activity. However, the prevention is a much better and more economical way to fight against cancer than treating an already advanced and often incurable disease.

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“…Recent studies have revealed that cancer‐associated miRNAs play important roles in tumorigenesis and may serve as diagnostic and prognostic biomarkers of various cancers . Increasing evidence indicates that miRNA deregulation has frequently been observed in the proliferation, differentiation, apoptosis, metastasis, and drug resistance of cancer cells . Although the precise function of individual miRNAs has not been characterized, biochemical and genetic studies have revealed that miRNAs regulate a variety of biological processes, such as cell proliferation, apoptosis, development, and differentiation…”

Section: Discussionmentioning

confidence: 99%

Micro ribonucleic acid (RNA)‐101 inhibits cell proliferation and invasion of lung cancer by regulating cyclooxygenase‐2

Zhang

et al. 2015

Thoracic Cancer

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BackgroundMicro ribonucleic acid (miR-101) can regulate the expression of cyclooxygenase-2 (COX-2) and participate in the pathogenesis of malignant tumors. This study investigates the effects of miRNA-101 and COX-2 in lung cancer and the impact of miR-101 on the proliferation and invasion of human lung cancer A549 cell line.MethodsThe expression of miR-101 in 20 separate lung cancer tissues was detected by real time polymerase chain reaction; COX-2 expression was also detected. A549 cells were transfected with miR-101 or negative control oligonucleotide duplex mimic (miR-NC). In vivo tumorigenesis abilities were detected in localized human lung cancer xeno-transplant models in BALB/c nude mice.ResultsMiR-101 expression was significantly lower and the level of COX-2 significantly higher in lung cancer tissues than in adjacent parenchyma (2.918 ± 1.006 vs. 5.953 ± 1.976, P = 0.001; 0.887 ± 0.260 vs. 0.355 ± 0.156, P = 0.001, respectively). Correlation analysis revealed that miR-101 negatively correlated with COX-2 in lung cancer tissues (R = −0.596, P = 0.002). Compared with A549-miR-NC cells, the expression of COX-2 was significantly decreased in A549 cells transfected with miR-101 (P < 0.001). The proliferation of A549 cells was markedly inhibited after transfection of miR-101. The in vivo tumor growth of A549 cells transfected with miR-101 was significantly slower than wide type A549 cells.ConclusionMiR-101 expression is decreased in lung cancer, inducing an increase in COX-2 level. Enforced expression of miR-101 can remarkably reduce the cell proliferation and invasion ability of lung cancer cells.

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Prognostic Value of Expression of MicroRNAs in Non-Small Cell Lung Cancer: A Systematic Review and Meta-Analysis

Abstract: Our meta-analysis provided the evidence that miR-21, miR-200c, miR-125b, miR-148b, miR-365, miR-124, miR-32, miR-146a, and miR-375 can act as prognostic biomarkers in NSCLC.

Cited by 35 publications

References 0 publications

Genetic variations in miR‐125 family and the survival of non‐small cell lung cancer in Chinese population

Genetic variations in miR‐125 family and the survival of non‐small cell lung cancer in Chinese population

The Role of Inflammation in Lung Cancer

Micro ribonucleic acid (RNA)‐101 inhibits cell proliferation and invasion of lung cancer by regulating cyclooxygenase‐2

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