Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Jakočiūnas, Tadas; Klitgaard, Andreas; Kontou, Eftychia Eva; Nielsen, Julie B.; Thomsen, Emil; Romero-Suarez, David; Blin, Kai; Kim, Young-Mo; Gin, Jennifer; Tong, Yaojun; Gotfredsen, Charlotte Held; Charusanti, Pep; Frandsen, Rasmus John Normand; Weber, Tilmann; Lee, Sang Yup; Jensen, Michael K.; Keasling, Jay D.

doi:10.1016/j.synbio.2020.01.004

Cited by 16 publications

(12 citation statements)

References 38 publications

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“…While transcriptomics yields gene expression data (i.e., activity of target genes, gene sequence data, and gene expression levels), proteomics and metabolomics approaches are increasingly being used for pathway analysis studies as they can measure protein translation and activity, respectively (Volke et al, 2019 ). Proteomics-guided approaches have been used to engineer polyketide biosynthesis platforms for aromatic compounds in yeast (Jakočiunas et al, 2020 ) and in vitro production of adipic acid (Hagen et al, 2016 ). In addition to this, metabolomics enables the assessment of pathway flux, carbon source diversion, and cofactor imbalance, which all contribute to the identification of pathway bottlenecks (Nielsen and Jewett, 2007 ; Zhao et al, 2020 , Volke et al, 2019 ).…”

Section: Omics-guided Biotechnologymentioning

confidence: 99%

Omics-Driven Biotechnology for Industrial Applications

Amer

Baidoo

2021

Front. Bioeng. Biotechnol.

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Biomanufacturing is a key component of biotechnology that uses biological systems to produce bioproducts of commercial relevance, which are of great interest to the energy, material, pharmaceutical, food, and agriculture industries. Biotechnology-based approaches, such as synthetic biology and metabolic engineering are heavily reliant on “omics” driven systems biology to characterize and understand metabolic networks. Knowledge gained from systems biology experiments aid the development of synthetic biology tools and the advancement of metabolic engineering studies toward establishing robust industrial biomanufacturing platforms. In this review, we discuss recent advances in “omics” technologies, compare the pros and cons of the different “omics” technologies, and discuss the necessary requirements for carrying out multi-omics experiments. We highlight the influence of “omics” technologies on the production of biofuels and bioproducts by metabolic engineering. Finally, we discuss the application of “omics” technologies to agricultural and food biotechnology, and review the impact of “omics” on current COVID-19 research.

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Section: Omics-guided Biotechnologymentioning

confidence: 99%

Omics-Driven Biotechnology for Industrial Applications

Amer

Baidoo

2021

Front. Bioeng. Biotechnol.

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“…In addition, we see more platform strains becoming available, providing sufficient precursor and co-factor supply, easing the discovery and production for specific product classes of interest. [171][172][173] Synthetic genetic circuits can be used to establish switches to specifically turn on BGC-derived pathway genes, allowing the de-coupling of growth from production. 174 For example, feedforward regulation has been described for many biosynthetic pathways of natural products, 175 allowing for dynamic regulation and the adaption to precursor availability and activating product export.…”

Section: Hit Generationmentioning

confidence: 99%

Section: Hit Generationmentioning

confidence: 99%

A Perspective on Synthetic Biology in Drug Discovery and Development—Current Impact and Future Opportunities

et al. 2021

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“…Polyketides are a diverse group of secondary metabolites produced by bacteria (type I, II, III), fungi (type I, II), and plants (type III). Polyketides exhibit various bioactive properties such as anticancer, antifungal, and antiviral, and serve as important resources for pharmaceutical development ( Jakočiūnas et al, 2020 ). Within the type III polyketides class, triacetic acid lactone, ρ-coumaric acid, naringenin, and resveratrol have been produced at high titers by fungal as well as bacterial hosts ( López et al, 2019 ).…”

Section: Production Of Non-native Chemicals In Yeast Cell Factoriesmentioning

confidence: 99%

Current Challenges and Opportunities in Non-native Chemical Production by Engineered Yeasts

Kim

Tran

Lee

2020

Front. Bioeng. Biotechnol.

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Yeasts are promising industrial hosts for sustainable production of fuels and chemicals. Apart from efficient bioethanol production, yeasts have recently demonstrated their potential for biodiesel production from renewable resources. The fuel-oriented product profiles of yeasts are now expanding to include non-native chemicals with the advances in synthetic biology. In this review, current challenges and opportunities in yeast engineering for sustainable production of non-native chemicals will be discussed, with a focus on the comparative evaluation of a bioethanol-producing Saccharomyces cerevisiae strain and a biodiesel-producing Yarrowia lipolytica strain. Synthetic pathways diverging from the distinctive cellular metabolism of these yeasts guide future directions for product-specific engineering strategies for the sustainable production of non-native chemicals on an industrial scale.

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Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Cited by 16 publications

References 38 publications

Omics-Driven Biotechnology for Industrial Applications

Omics-Driven Biotechnology for Industrial Applications

A Perspective on Synthetic Biology in Drug Discovery and Development—Current Impact and Future Opportunities

Current Challenges and Opportunities in Non-native Chemical Production by Engineered Yeasts

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