Programmed cell death in the germline

Baum, Jason; George, J.P. St.; McCall, Kimberly

doi:10.1016/j.semcdb.2004.12.008

Cited by 117 publications

(98 citation statements)

References 123 publications

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“…As it has been demonstrated before, the process of programmed cell death is activated and implemented during Diptera germarium, mid-and late-oogenesis (Drummond-Barbosa and Spradling, 2001;Nezis et al, 2000;2001;2005;2006a;2006b;2006c, for reviews see McCall, 2004;Baum et al, 2005). Follicular atresia, a distinct pattern of developmentally regulated cell death, has been sporadically observed during Diptera mid-oogenesis (Giorgi and Deri, 1976;Nezis et al, 2000;2006a;Uchida et al, 2004), or massively induced in response to (a) nutritional deprivation, (b) ecdysone signalling malfunction, (c) treatment with chemotherapeutic drugs (i.e.…”

Section: Introductionmentioning

confidence: 91%

“…The atretic egg chambers contain degenerated nurse cells, mainly characterized by condensed chromatin, fragmented DNA and disorganized actin cytoskeleton network (Buszczak et al, 1999;Chao and Nagoshi, 1999;DeLorenzo et al, 1999;Soller et al, 1999;Nezis et al, 2000;2006a). Nurse and follicle cell death programs are also required for the normal maturation of the developing follicles during the late stages of Drosophila, Bactrocera oleae and C. capitata oogenesis (Cavaliere et al, 1998;Foley and Cooley, 1998;Nezis et al, 2000;2001;2006b;2006c, for reviews see McCall, 2004Baum et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)

Velentzas¹,

Nezis²,

Stravopodis³

et al. 2007

Int. J. Dev. Biol.

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In the present study, we describe novel features of programmed cell death in developing egg chambers occurring during mid-and late-oogenesis of the medfly Ceratitis capitata. During mid-oogenesis, the spontaneously degenerated egg chambers exhibit typical characteristics of apoptotic cell death. Their nurse cells contain fragmented DNA and fragmented actin, as revealed by TUNEL assay and immunolabelling, respectively. In vitro caspase activity assays and immunostaining procedures demonstrated that the atretic egg chambers acquired high levels of caspase activity. Distinct features of autophagic cell death were also observed during C. capitata mid-oogenesis, as revealed by the monodansylcadaverine staining approach and ultrastructural examination performed by transmission electron microscopy. Additionally, atretic egg chambers exhibit an upregulation of lysosomal proteases, as demonstrated by a procathepsin L immunolabelling procedure. At the late stages of C. capitata oogenesis, apoptosis and autophagy coexist, manifesting cell death features that are similar to the ones mentioned above, being also chaperoned by the involvement of an altered cytochrome c conformational display. We propose that apoptosis and autophagy operate synergistically during C. capitata oogenesis for a more efficient elimination of the degenerated nurse cells and abnormal egg chambers.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)

Velentzas¹,

Nezis²,

Stravopodis³

et al. 2007

Int. J. Dev. Biol.

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“…Programmed cell death is a component of both invertebrate and vertebrate germline development (reviewed in Baum et al 1 and in Tilly 2 ). In mammals there is a wave of oocyte cell death prior to or around birth, with continuing cell death during postnatal life.…”

Section: Introductionmentioning

confidence: 99%

“…Insect germline cell death has been studied in several species, including Ceratitis capitata, Dacus oleae, and most extensively in Drosophila melanogaster. 1 The female germline of these insects is organized into egg chambers consisting of an oocyte and nurse cells that provide the growing oocyte with macromolecules, including proteins, mRNA, and organelles. During oogenesis, the nurse cells gradually transport maternal components to the oocyte, and then finally extrude all their remaining cytoplasmic contents into the oocyte, after which the nurse cell remnants die.…”

Section: Introductionmentioning

confidence: 99%

Illuminating the role of caspases during Drosophila oogenesis

Mazzalupo

Cooley

2006

Cell Death Differ

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Cell death is a prominent feature of animal germline development. In Drosophila, the death of 15 nurse cells is linked to the development of each oocyte. In addition, females respond to poor environmental conditions by inducing egg chamber death prior to yolk uptake by the oocyte. To study these two forms of cell death, we analyzed caspase activity in the germline by expressing a transgene encoding a caspase cleavage site flanked by cyan fluorescent protein and yellow fluorescent protein. When expressed in ovaries undergoing starvation-induced apoptosis, this construct was an accurate reporter of caspase activity. However, dying nurse cells at the end of normal oogenesis showed no evidence of cytoplasmic caspase activity. Furthermore, although expression of the caspase inhibitors p35 or Drosophila inhibitor of apoptosis protein 1 blocked starvation-induced death, it did not affect normal nurse cell death or overall oogenesis in well-fed females. Our data suggest that caspases play no role in developmentally programmed nurse cell death.

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“…Apoptosis is indispensable for normal spermatogenesis (Baum et al, 2005). High levels of germ cell apoptosis occur in spermatogonia (Clermont, 1962) and spermatocytes (Blanco-Rodriguez et al, 2003 …”

Section: Introductionmentioning

confidence: 99%

Regulated expression of the ubiquitin protein ligase, E3^Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis

Liu

Miao

Xia

et al. 2007

Developmental Dynamics

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A ubiquitin protein ligase (E3), E3Histone /LASU1 (Mule/ARF-BP1/HUWE1), was recently identified that mediates ubiquitination of core histones, the Mcl-1 anti-apoptotic protein, and the p53 tumor suppressor protein. However, the expression of E3 Histone /LASU1 remains poorly studied. Because we identified E3 Histone /LASU1 from the testis, we explored its regulation during spermatogenesis. In the first wave of rat spermatogenesis, E3Histone /LASU1 mRNA and protein had peak expression at days 10 and 20, respectively, and decreased with age. Consistent with these findings, immunohistochemistry revealed that E3 Histone /LASU1 was highly expressed in nuclei from spermatogonia to mid-pachytene spermatocytes. There was no obvious staining in spermatids, when histones are ubiquitinated and degraded.

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Programmed cell death in the germline

Cited by 117 publications

References 123 publications

Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)

Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)

Illuminating the role of caspases during Drosophila oogenesis

Regulated expression of the ubiquitin protein ligase, E3^Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis

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Programmed cell death in the germline

Cited by 117 publications

References 123 publications

Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)

Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)

Illuminating the role of caspases during Drosophila oogenesis

Regulated expression of the ubiquitin protein ligase, E3Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis

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Regulated expression of the ubiquitin protein ligase, E3^Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis