Prolactin affects bovine oocytes through direct and cumulus-mediated pathways

Lebedeva, I.; Сингина, Г. Н.; Волкова, Н. А.; Vejlsted, Morten; Zinovieva, N. A.; Schmidt, M.

doi:10.1016/j.theriogenology.2014.08.005

Cited by 14 publications

(23 citation statements)

References 54 publications

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“…Our data have demonstrated that both PRL and GH affect the morphology of M-II chromosomes in CEOs in a biphasic dose-dependent manner, which is typical for actions of these hormones in different cell systems ( Ilondo et al, 1994 ; Hodson et al, 2010 ; Lebedeva et al, 2014b ). A bell-shaped pattern of dose-response curves for PRL and GH is usually attributed to the two-site mechanism of the hormone binding to their receptors involving dimerization of these latter and self-antagonism at high concentrations when monomeric hormone-receptor complexes become predominant ( Kelly et al, 1994 ).…”

Section: Discussionsupporting

confidence: 51%

“…The protein expression of PRL and GH receptors in cumulus cells surrounding in vitro matured oocytes was detected by immunocytochemistry as described previously for freshly isolated bovine CEOs ( Lebedeva et al, 2014b ). Briefly, following 20 h of maturation, CEOs were washed twice in PBS containing 0.2% (w/v) bovine serum albumin (PBS-BSA) and fixed with 4% (w/v) paraformaldehyde in PBS for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…For detection of PRL and GH receptors, the specimens were incubated respectively with mouse anti-PRL receptor monoclonal antibody MA1-610 (Thermo Scientific, Rockford, IL, USA; 1:50 dilution) or mouse anti-GH receptor monoclonal antibody MAB 263 (Abcam, Cambridge, MA, USA; 1:50 dilution) overnight at 4°C. The primary antibody MA1-610 reacts both with long and short PRL receptor isoforms and detects the receptor in tissues of different mammalian species including bovine cumulus cells ( Lebedeva et al, 2014b ). The antibody MAB 263 has been extensively validated for immunohistochemical studies in various types of tissues including bovine ovary ( Kölle et al, 1998 ).…”

Section: Methodsmentioning

confidence: 99%

“…The available evidence points to participation of two closely-related hormones, prolactin (PRL) and growth hormone (GH), in modulation of the mammalian oocyte maturation and developmental competence ( Izadyar et al, 1996 ; Bole-Feysot et al, 1998 ; Hull and Harvey, 2002 ; Lebedeva et al, 2014b ). Receptors of PRL and GH or their mRNA have been detected in oocytes and surrounding cumulus cells of different species including cows ( Bevers and Izadyar, 2002 ; Marchal et al, 2003 ; Picazo et al, 2004 ; Lebedeva et al, 2014b ). Both hormones can modulate the mitochondrial activity and/or calcium homeostasis in bovine oocytes maturing in vitro ( Kuzmina et al, 1999 , 2007 ), indicating the hormonal implication in processes modified by aging.…”

Section: Introductionmentioning

confidence: 99%

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Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

et al. 2015

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General senescence of the adult organism is closely connected with reproductive one. Meanwhile, the age-related reduction in the female fertility is primarily associated with a decline in the gamete quality. Molecular and cellular changes in oocytes of old mammalian females are very similar to those occurring during aging of matured ova of their young counterparts, suggesting similarities in underlying mechanisms. The aim of the present work was to study actions of two related pituitary hormones, prolactin (PRL) and growth hormone (GH), on age-associated modifications of metaphase-II (M-II) chromosomes in bovine oocytes using a model of the prolonged culture. We analyzed: (1) effects of PRL and GH on abnormal changes in the chromosome morphology in aging matured oocytes and the role of cumulus cells in these effects and (2) signaling pathways involved in the hormone actions. During the prolonged culture of oocytes, a gradual rise in the frequency of destructive modifications of M-II chromosomes was revealed. In the case of cumulus-enclosed oocytes (CEOs), PRL and GH exerted dose-dependent biphasic effects on the frequency of these modifications. Both PRL (50 ng/ml) and GH (10 ng/ml) decelerated the abnormal chromosome changes in CEOs, but did not affect the chromosome configuration in denuded oocytes. Concurrently, the presence of PRL and GH receptors in cumulus cells surrounding matured oocytes was demonstrated. Attenuating effects of both hormones on the chromosome modifications in aging CEOs were abolished by PP2 (an inhibitor of Src-family tyrosine kinases), triciribine (an inhibitor of Akt kinase), and calphostin C (a protein kinase C inhibitor). Our findings indicate that PRL and GH can exert the similar decelerating action on age-associated alterations in the M-II chromosome morphology in bovine ova, which is mediated by cumulus cells and may be related to activation of Src-family tyrosine kinases as well as Akt- and protein kinase C-dependent signal pathways.

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Section: Discussionsupporting

confidence: 51%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

et al. 2015

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show abstract

“…Optimization of culture media through supplementation with hormones, proteins and growth factors is one of the main current fields of research in in vitro blastocyst production; its main goal is to improve both efficiency of the technique and embryo quality [8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%