Prolidase activity and oxidative status in patients with thalassemia major

Çakmak, Alpay; Söker, Murat; Ahmet, Koc; Aksoy, Nurten

doi:10.1002/jcla.20361

Cited by 26 publications

(25 citation statements)

References 39 publications

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“…In addition, a significant decrease of TAC has been reported in thalassemia patients compared to controls [42,50]. TAC was also lower in thalassemia patients with DFO chelation therapy compared to those without DFO [50] while Cakmak et al [51] reported no significant differences in TAC between thalassemia and control groups, in spite of increased level of oxidant status and oxidative stress index. These findings imply that oxidant-antioxidant balance was disturbed in β-TM and there is enhanced oxidative stress with decreased antioxidant defense irrespective of bilirubin levels.…”

Section: Discussionmentioning

confidence: 97%

Ischemia-modified albumin as a marker of vascular dysfunction and subclinical atherosclerosis in β-thalassemia major

et al. 2017

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Background: Ischemia-modified albumin (IMA) is an altered type of serum albumin that forms under conditions of oxidative stress and an independent predictor of major adverse cardiovascular events. Objectives: To measure the levels of IMA in 45 children and adolescents with β-thalassemia major (β-TM) compared with 30 healthy controls and assess its relation to lipid peroxidation, vascular complications and subclinical atherosclerosis. Methods: β-TM patients without symptoms of heart disease were studied focusing on transfusion history, chelation therapy, serum ferritin, malondialdehyde (MDA) and IMA levels. Echocardiography was performed and carotid intima media thickness (CIMT) was assessed. Results: IMA and MDA levels were significantly higher in β-TM patients compared with controls (p < 0.001). IMA was higher among patients with heart disease, pulmonary hypertension risk and serum ferritin ≥2500 µg/l than those without. TM patients compliant to chelation had significantly lower IMA levels. IMA levels were positively correlated to MDA and CIMT while negatively correlated to ejection fraction and fractional shortening. Conclusion: Our results highlight the role of oxidative stress in the pathophysiology of vascular complications in thalassemia. IMA could be useful for screening of β-TM patients at risk of cardiopulmonary complications and atherosclerosis because its alteration occurs in early subclinical disease.

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Section: Discussionmentioning

confidence: 97%

Ischemia-modified albumin as a marker of vascular dysfunction and subclinical atherosclerosis in β-thalassemia major

et al. 2017

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“…The homogenate was centrifuged at 2,800 × g for 10 min at 4°C (20) . Total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured in supernatant and ocular tissue samples as described previously (21) . Briefly, TAS was measured spectrophotometrically using a commercial kit ( …”

Section: Analysis Of Oxidative Stress Markers In the Aqueous Humor Anmentioning

confidence: 99%

Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats

Göncü¹,

Oğuz²,

Sezen³

et al. 2016

Arquivos Brasileiros De Oftalmologia

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“…In addition to collagen turnover, oxidative status and oxidative stress have also been shown to correlate with prolidase activity levels under certain conditions (Aslan et al. 2007; Cakmak et al. 2009, 2010; Selek et al.…”

Section: Prolidase As a Marker For Diseasementioning

confidence: 99%

“…The coupling of oxidative stress with the lack of antioxidant response could explain why prolidase is elevated in this situation. In thalassaemia major, an autosomal recessive blood disorder characterized by widening of the bone marrow cavities and decreased bone mass, elevated prolidase activity has been documented (Cakmak et al. 2010).…”

Section: Prolidase As a Marker For Diseasementioning

confidence: 99%

Prolidase function in proline metabolism and its medical and biotechnological applications

Kitchener

Grunden

2012

J Appl Microbiol

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Summary Prolidase is a multifunctional enzyme that possesses the unique ability to degrade imidodipeptides in which a proline or hydroxyproline residue is located at the C‐terminal end. Prolidases have been isolated from archaea and bacteria, where they are thought to participate in proline recycling. In mammalian species, prolidases are found in the cytoplasm and function primarily to liberate proline in the final stage of protein catabolism, particularly during the biosynthesis and degradation of collagen. Collagen comprises nearly one‐third of the total protein in the body, and it is essential in maintaining tissue structure and integrity. Prolidase deficiency (PD), a rare autosomal recessive disorder in which mutations in the PEPD gene affect prolidase functionality, tends to have serious and sometimes life‐threatening clinical symptoms. Recombinant prolidases have many applications and have been investigated not only as a possible treatment for PD, but also as a part of anti‐cancer strategies, a component of biodecontamination cocktails and in the dairy industry. This review will serve to discuss the many in vivo functions of procaryotic and eucaryotic prolidases, as well as the most recent advances in therapeutic and biotechnological application of prolidases.

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Prolidase activity and oxidative status in patients with thalassemia major

Abstract: Significant increases in prolidase activity in patients with thalassemia major may constitute a key parameter in demonstrating a disorder of the collagen metabolism.

Cited by 26 publications

References 39 publications

Ischemia-modified albumin as a marker of vascular dysfunction and subclinical atherosclerosis in β-thalassemia major

Ischemia-modified albumin as a marker of vascular dysfunction and subclinical atherosclerosis in β-thalassemia major

Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats

Prolidase function in proline metabolism and its medical and biotechnological applications

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