Proliferation and Differentiation of Hematopoietic Cells and Preservation of Immune Functions

Hayashi, Osamu

doi:10.5772/48322

Cited by 3 publications

(2 citation statements)

References 60 publications

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“…Such results have already been reported in agreement with (Neyrinck et al, 2017) Altogether, those data indicate an improvement of the immune function of mice after oral intake of SP. Furthermore, other studies also showed anticancer effects of the bioactive compound phycocyanin from spirulina on differentiation and morphological and cytochemical changes of human myeloid leukemia cell lines, U937 and HL-60 cells as well as the enhanced the expression of monocytic surface antigens CD11b and CD14 on human premonocytic U937 cells (Hayashi 2012), which are generally used for the studies of cell differentiation (Hayashi 2012).…”

Section: Effect Of Sp Treatment On Alt and Ast Activitiesmentioning

confidence: 99%

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Medhat

El‐Sheekh²,

Abo-Shady³

et al. 2019

Journal of Cancer and Biomedical Research

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Spirulina platensis (SP) is a blue-green alga used as a dietary supplement as it possesses antioxidant, anti-inflammatory and hepatoprotective effects with clinical importance in several disorders. The aim of this study was to evaluate the ameliorating effects of SP extract on toxicity induced mice by treatment with the anticancer drug cyclophosphamide (CTX). To this end, SP was cultured under optimal conditions of growth at pH 9 for 9 days using Zarrouk , s medium. The active ingredients including pigments, phycobiliproteins and total soluble proteins were estimated in SP suspensions which were then dried to a powder form. To assess antitoxicity, adult male albino mice were treated with interperitoneal (i.p.) injection of PBS, single dose of CTX (4mg/mouse), CTX (i.p.) followed by subcutaneous (s.c.) injection of G-CSF (5μg/mouse) daily for 5 consecutive days or CTX (i.p.) followed by oral administration of 0.5 gm/mouse SP extract for 7 consecutive days. After 7 days of initial treatment with CTX, mice were sacrificed; blood, spleen, bone marrow and liver were harvested to assess CBC, total count for spleen and bone marrow and liver for biochemical analysis. The results showed that SP showed the highest yield of dry weight and pigment content C-phycocyanin (CPC), allophycocyanin (APC), phycoerthrin (PE) and total phycobiliproteins (phycobilins) at the 9 th day of growth at pH 9 in culture. Oral administration of SP induced amelioration of CTX induced leucopenia in blood, bone marrow and spleen comparable to those of G-CSF. It also ameliorated the dysfunction in the liver enzymes ALT and AST. Interestingly, however, SP showed higher antioxidant effects than those of G-CSF as reflected by the higher activities of the anti-oxidant MDA and GSH. Conclusion: SP showed potent antitoxic effect through antioxidant activity and thus could be a useful co-adjuvant agent against chemotherapeutic drugs toxicity including cyclophosphamide. Protective effect of Spirulina platensis on Cyclophophamide …El-Sheekh et al., 2019

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Section: Effect Of Sp Treatment On Alt and Ast Activitiesmentioning

confidence: 99%

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Medhat

El‐Sheekh²,

Abo-Shady³

et al. 2019

Journal of Cancer and Biomedical Research

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“…Phycocyanin (phyco) is a photosynthetic pigment protein of Spirulina platensis. Its health promoting and medicinal effects such as its antioxidant, anti-inflammatory, anticancer, hepatoprotective, neuroprotective, and immunomodulatory effects have also been reported [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Immuno-regulatory and anti-inflammatory actions of phycocyanin on Caco-2/U937 cells co-culture as a model of the intestinal barrier

Yoshimoto¹,

Okada²,

Hayashi

2019

FFHD

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Background: Phycocyanin, a photosynthetic pigment protein of cyanobacteria Spirulina platensis, has been used as a health or functional food because of its health promoting and medicinal effects such as its antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects.Methods: Under the co-culture system using a human colorectal cancer cell line, Caco-2 cells, and differentiated U937 macrophages in the presence of sodium butyrate (SB) and/or lipopolysaccharide (LPS), immune-regulatory and anti-inflammatory activities of phycocyanin were examined.Results: Phycocyanin attenuated a certain damage in the tight junction of co-cultured Caco-2 cells induced by LPS in the presence of SB, and suppressed IL-6 and IL-8 and conversely enhanced TGF-β1 productions. Phagocytic and bactericidal abilities of the differentiated U937 cells were also regulated under the co-cultivation.Conclusions: Phycocyanin contributes to protecting against inflammation and to regulating macrophages in the mucosal immune responses partly through release of cytokines in the presence of butyrate.Keywords: Spirulina, phycocyanin, Caco-2 cells, U937 cells, IL-6, IL-8, TGF-β1

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Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway

et al. 2017

Sci Rep

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Our aim was to investigate the effects of phycocyanin (PC) on bleomycin (BLM)-induced pulmonary fibrosis (PF). In this study, C57 BL/6 wild-type (WT) mice and toll-like receptor (TLR) 2 deficient mice were treated with PC for 28 days following BLM exposure. Serum and lung tissues were collected on days 3, 7 and 28. Data shows PC significantly decreased the levels of hydroxyproline (HYP), vimentin, surfactant-associated protein C (SP-C), fibroblast specific protein-1 (S100A4) and α-smooth muscle actin (α-SMA) but dramatically increased E-cadherin and podoplanin (PDPN) expression on day 28. Moreover, PC greatly decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) at the earlier time. Reduced expression of key genes in the TLR2 pathway was also detected. Compared with WT mice, TLR2-deficient mice exhibited less injury, and the protective effect of PC was partly diminished in this background. These data indicate the anti-fibrotic effects of PC may be mediated by reducing W/D ratio, MPO, IL-6, TNF-α, protecting type I alveolar epithelial cells, inhibiting fibroblast proliferation, attenuating epithelial-mesenchymal transitions (EMT) and reducing oxidative stress. The TLR2-MyD88-NF-κB pathway plays an important role in PC-mediated reduction in pulmonary fibrosis.

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Proliferation and Differentiation of Hematopoietic Cells and Preservation of Immune Functions

Cited by 3 publications

References 60 publications

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice

Immuno-regulatory and anti-inflammatory actions of phycocyanin on Caco-2/U937 cells co-culture as a model of the intestinal barrier

Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway

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