Prolificacy Detection in Egyptian Sheep using RFLP-Specific PCR

Abulyazid, I.; Abdalla, Moustafa; Sharada, H. M.; Saleh, Hatem Mohamed; Hassanin, W. F.

doi:10.21608/eajbsc.2011.16139

Egyptian Academic Journal of Biological Sciences. C, Physiology

2011

DOI: 10.21608/eajbsc.2011.16139

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Prolificacy Detection in Egyptian Sheep using RFLP-Specific PCR

I. Abulyazid

Moustafa Abdalla²,

H. M. Sharada³

et al.

Abstract: Booroola gene (FecB) was the first major gene for prolificacy identified in sheep. In this study twenty two (11 twin producing female, 7 single lamb producing female, and 4 male) crossbred sheep were tested for the presence of the FecB mutation of BMPR1B. The females were selected for their twin production in three repetitive production cycles while the males were selected for being produced from prolific females as above. Forced restriction PCR of the FecB gene, 190 base pair (bp) was amplified using specific… Show more

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Cited by 5 publications

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Investigation of the Booroola Gene in Egyptian Ewes with Different Reproductive Status Using PCR- RFLP

Ahmed¹,

M.²,

Kandiel

et al. 2016

NIDOC-ASRT

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VULATION CAN BE regulated by a group of genes, termed as fecundity (Fec) genes. The aim of this study is to identify genetic polymorphism in the Booroola (FecB) gene in Barki, Rahmani, and Ossimi sheep breeds with different physiological status diagnosed by ultrasound. Accordingly, animals were early classified into three groups: carrying single fetus, twin fetuses and non-pregnant by ultrasonographic examination. Demonstration of the fetal number was available as early as Day 35-40 post-mating transrectally, and transabdominally. The fetal viability was checked through heart examination with M-Mode. Genomic DNA was extracted from blood samples of the total number of sheep and two primers were used to amplify 190 and 140 bp fragments of FecB gene. The amplified fragments were digested using AvaII restriction enzyme. All sheep groups were non carriers for the FecB mutation and gave a 190 bp band (++) and 140 bp band (Fec++) for primer 1 and 2, respectively. In conclusion, no genetic polymorphism was detected in the three Egyptian sheep breeds in relation to pregnancy with single or twin fetuses. The study could be continued to search for other major genes.

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Investigation of the Booroola Gene in Egyptian Ewes with Different Reproductive Status Using PCR- RFLP

Ahmed¹,

M.²,

Kandiel

et al. 2016

NIDOC-ASRT

View full text Add to dashboard Cite

show abstract

Genetic polymorphism detection in bone morphogenetic protein 15 (BMP15) gene related to fecundity in two Egyptian sheep breeds

Hassan

Nassar

2017

BIOTEC ANIM HUSB

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This study was intended to detect the polymorphism of bone morphogenetic protein 15 (BMP15) gene that can act as marker influencing fertility for increasing litter size in Egyptian sheep breeds (191 Saidi and 145 Ossimi females). In this study, the mean litter size, showed highly significant between Saidi and Ossimi sheep breads, however, litter size of Saidi white sheep was significantly decreased compared to black and brown. Blood samples were collected from 19 Saidi and 13 Ossimi female and then genomic DNA was extracted. A portion of bone morphogenetic protein 15 (BMP15) gene, 310 bp was amplified using specific primers, and was sequenced and analyzed to clarify the phylogenetic relationship of Egyptian breed sheep. The data suggested that the gene shared a similarity in sequence compared to 9 accession numbers of Ovis aries found in GenBank. Molecular phylogenetic analyses were performed based on nucleotide sequences in order to examine the position of the Egyptian breeds among many other sheep breeds. The results indicate that 5 accession numbers of Ovis aries are closely related with Ossimi and Saidi female that produce single or twins lamb in UPGMA analysis. In addition, PCR-RFLP method using Pst1 and Msp1 restriction enzymes was used to mask polymorphisms of partial exon 2 in 18 female sheep. Results showed that FecX gene was monomorphic and disagreement with litter size, therefore, it is indispensable to survey other gene in order to establish marker assisted selection technique.

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FSHR (exon 10) gene polymorphisms and its association with fertility trait in Egyptian Ossimi sheep

et al. 2019

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For the association between of Follicle stimulating hormone receptor (FSHR) gene (partial part of exon 10) polymorphisms and litter size trait in Egyptian Ossimi sheep, polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and DNA sequencing techniques were developed. Fifty female Ossimi sheep reared under Egyptian conditions were selected according to their litter size. DNA from blood samples of these animals was isolated to amplify 250-bp of the FSHR gene influencing litter size production trait in sheep. Based on litter size, 50 animals were selected from the highest to the lowest litter size productivity during three seasons. PCR-SSCP analysis of the FSHR gene (250-bp) showed two various genotypes AA and AB with frequencies 0.64 and 0.36, respectively. The frequencies of the A and B alleles were 0.82 and 0.18, respectively. PCR fragment of FSHR gene (191-bp) was sequenced only in the high and low litter size productivity animals (GenBank accession numbers from MG973191 to MG973207, sequentially). The result indicated that 6SNPs

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Prolificacy Detection in Egyptian Sheep using RFLP-Specific PCR

Cited by 5 publications

References 9 publications

Investigation of the Booroola Gene in Egyptian Ewes with Different Reproductive Status Using PCR- RFLP

Investigation of the Booroola Gene in Egyptian Ewes with Different Reproductive Status Using PCR- RFLP

Genetic polymorphism detection in bone morphogenetic protein 15 (BMP15) gene related to fecundity in two Egyptian sheep breeds

FSHR (exon 10) gene polymorphisms and its association with fertility trait in Egyptian Ossimi sheep

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