Prolongation of Anti-Inflammatory Activity of Glucocorticosteroids Encapsulated in Large Oligolamellar Liposomes in Treatment of Arthritis in Rabbits

Rosenberg, O.; Seiliev, A. A.; Shul'ga, A E; Volchkov, Vladimir

doi:10.21103/article7(2)_oa9

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…The retention time of the compound was found to be 36.7 s, and it eluted from along with the eluents from KACC 2 extract. Prednisolone hemisuccinate has been known to possess antiinflammatory activity [22]. The retention time was found to be 37.02, and the extract from KACC 2 was found to possess the compound.…”

Section: Gc/ms Analysismentioning

confidence: 95%

Anticancer Activity of Micro-Algae Extract on Human Cancer Cell Line (Mg-63)

James

Thomas

2019

Asian J Pharm Clin Res

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Objective: The objective of the present study is to assess the anticancer effect of limnic algae extract of KACC 2 (Karunya Algae Culture Collection) and KACC 23 against MG-63 cell line.Methods: Bioactive compounds were extracted from the algae culture by Soxhlet extraction method using methanol as solvent, and the extract was checked for its anticancer activity. Polyphenols were estimated using Folin-Ciocalteu reagent method. The constituents of the extract were analyzed using gas chromatography-mass spectrometry (GC-MS).Results: The results showed that KACC 2 had more phenolic content than KACC 23. The half maximal inhibitory concentration value for KACC 2 and 23 was found as 0.386 μl/ml and 0.285 μl/ml of extracts necessary for the 50% of cell death. GC-MS analysis revealed that the compounds with possible therapeutic effects are quercetin, stigmast-4-en-3-one, epoxygedunin, which has got anticancer activity.Conclusion: These observations point out that the algae extract of KACC 2 and KACC 23 has anticancer property and the phytoconstituents contributed to the anticancer effects.

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Section: Gc/ms Analysismentioning

confidence: 95%

Anticancer Activity of Micro-Algae Extract on Human Cancer Cell Line (Mg-63)

James

Thomas

2019

Asian J Pharm Clin Res

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“…[14] For instance, the intra-articular administration of prednisolone and hydrocortisone encapsulated into liposomes prolonged the anti-inflammatory effect by 7-8 days compared to 1 day for free glucocorticoids in arthritic rabbits. [41] In fact, in order to improve the therapeutic action of several drugs as well as to enhance their site-specific distribution, PEGylated liposomes were extensively investigated in several preclinical and clinical studies. [14] Therefore, the developed IL-23 axis targeted liposomes are a promising therapeutic approach not only for RA but also other ADs.…”

Section: Discussionmentioning

confidence: 99%

Biofunctionalized Liposomes to Monitor Rheumatoid Arthritis Regression Stimulated by Interleukin‐23 Neutralization

Lima

Campos

Cunha

et al. 2020

Adv Healthcare Materials

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Even after the revolution of rheumatoid arthritis (RA) treatment with biologic agents, this debilitating disease remains a major clinical problem. The outstanding outcomes of the systemic administration of antibodies (Abs) are narrowed by the risk of serious side effects and limited efficacy due to their short half‐life. Interleukin‐23 (IL‐23) is a crucial pro‐inflammatory cytokine involved in inflammation that potently enhances the generation of T‐helper type‐17 (Th17) cells. Hence, in this work, anti‐IL‐23 Abs are immobilized at the surface of liposomes to increase their therapeutic efficacy, being gold nanoparticles (AuNPs) incorporated to allow monitoring the biodistribution of the liposomes after systemic administration as well as due to their anti‐inflammatory and antioxidant effects. A stable monodispersed liposomes’ suspension with around 130 nm is produced and efficiently biofunctionalized with anti‐IL‐23 Abs. IL‐23 capture and neutralization capacity are confirmed using activated macrophages. Biological assays demonstrate their hemocompatibility and cytocompatibility with human articular chondrocytes, macrophages, and endothelial cells. Moreover, the neutralization of IL‐23 by the biofunctionalized liposomes efficiently decreases the production of IL‐17A by peripheral blood mononuclear cells of healthy donors and RA patients who are activated to Th17 differentiation. Therefore, the developed formulation may be a promising strategy to treat RA.

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