Prolonged outbreak of Serratia marcescens in Tartu University Hospital: a case–control study

Adamson, Vivika; Mitt, Piret; Pisarev, Heti; Metsvaht, Tuuli; Telling, Kaidi; Naaber, Paul; Maimets, Matti

doi:10.1186/1471-2334-12-281

Cited by 24 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(151,152). We did not see any significant differences between non-Serratia LOS and LOS with S. marcescens.…”

Section: Discussioncontrasting

confidence: 53%

The faecal flora: a source of healthcare-associated infections and antibiotic resistance

Samuelsson¹

2013

View full text Add to dashboard Cite

“…(151,152). We did not see any significant differences between non-Serratia LOS and LOS with S. marcescens.…”

Section: Discussioncontrasting

confidence: 53%

The faecal flora: a source of healthcare-associated infections and antibiotic resistance

Samuelsson¹

2013

View full text Add to dashboard Cite

“…was also found to be present in higher abundance in CD. The phylogenetic microarray targets mainly S. marcescens , which is considered as an opportunistic pathogen able to cause invasive infections (sepsis, meningitis, pneumonitis) in neonates [45,46]. These bacteria may impair the intestinal integrity, but their possible role in CD remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Duodenal microbiota composition and mucosal homeostasis in pediatric celiac disease

et al. 2013

View full text Add to dashboard Cite

BackgroundCeliac disease (CD) is an autoimmune disorder of the small intestine which is triggered by dietary gluten in genetically predisposed (HLA-DQ2/DQ8 positive) individuals. Only a fraction of HLA-DQ2/DQ8 positive individuals develop CD indicating that other factors have a role in the disorder. Several studies have addressed intestinal microbiota aberrancies in pediatric CD, but the results are inconsistent. Previously, we demonstrated that pediatric CD patients have lower duodenal expression of TLR2 and higher expression of TLR9 as compared to healthy controls (HC) indicating that microbiota may have a role in CD.MethodsWe used bacterial phylogenetic microarray to comprehensively profile the microbiota in duodenal biopsies of CD (n = 10) and HC (n = 9) children. The expression of selected mucosa-associated genes was assessed by qRT-PCR in CD and HC children and in treated CD adults (T-CD, n = 6) on gluten free diet.ResultsThe overall composition, diversity and the estimated microbe associated molecular pattern (MAMP) content of microbiota were comparable between CD and HC, but a sub-population profile comprising eight genus-like bacterial groups was found to differ significantly between HC and CD. In HC, increased TLR2 expression was positively correlated with the expression of tight junction protein ZO-1. In CD and T-CD, the expression of IL-10, IFN-g and CXCR6 were higher as co5mpared to HC.ConclusionsThe results suggest that microbiota and altered expression of mucosal receptors have a role in CD. In CD subjects, the increased expression of IL-10 and IFN-g may have partly resulted from the increased TLR9 expression and signaling.

show abstract

“…4 However, the organism is capable of causing nosocomial infections in other body systems, most notably respiratory and urinary tract infection, meningitis, bacteremia and different types of wound infection. [5][6][7] Serratia species cause outbreaks in neonatal intensive care units, 8,9 including necrotizing enterocolitis, and have been associated with significant morbidity and mortality in children. 10 However, recent studies showed that 65% of all Serratia infections were community-based, with S. marcescens being the most commonly isolated species, accounting for 92% of all isolated Serratia.…”

Section: Introductionmentioning

confidence: 99%

Serratia marcescensis injurious to intestinal epithelial cells

et al. 2014

View full text Add to dashboard Cite

Diarrhea causes substantial morbidity and mortality in children in low-income countries. Although numerous pathogens cause diarrhea, the etiology of many episodes remains unknown. Serratia marcescens is incriminated in hospital-associated infections, and HIV/AIDS associated diarrhea. We have recently found that Serratia spp. may be found more commonly in the stools of patients with diarrhea than in asymptomatic control children. We therefore investigated the possible enteric pathogenicity of S. marcescens in vitro employing a polarized human colonic epithelial cell (T84) monolayer. Infected monolayers were assayed for bacterial invasion, transepithelial electrical resistance (TEER), cytotoxicity, interleukin-8 (IL-8) release and morphological changes by scanning electron microscopy. We observed significantly greater epithelial cell invasion by S. marcescens compared to Escherichia coli strain HS (p = 0.0038 respectively). Cell invasion was accompanied by reduction in TEER and secretion of IL-8. Lactate dehydrogenase (LDH) extracellular concentration rapidly increased within a few hours of exposure of the monolayer to S. marcescens. Scanning electron microscopy of S. marcescens-infected monolayers demonstrated destruction of microvilli and vacuolization. Our results suggest that S. marcescens interacts with intestinal epithelial cells in culture and induces dramatic alterations similar to those produced by known enteric pathogens.

show abstract

Prolonged outbreak of Serratia marcescens in Tartu University Hospital: a case–control study

Cited by 24 publications

References 21 publications

The faecal flora: a source of healthcare-associated infections and antibiotic resistance

The faecal flora: a source of healthcare-associated infections and antibiotic resistance

Duodenal microbiota composition and mucosal homeostasis in pediatric celiac disease

Serratia marcescensis injurious to intestinal epithelial cells

Contact Info

Product

Resources

About