Prolonged Regional Nerve Blockade by Controlled Release of Local Anesthetic from a Biodegradable Polymer Matrix

Masters, David B.; Berde, Charles B.; Dutta, Sanjoy; Griggs, Chauncey T.; Hu, D.; Kupsky, William J.; Langer, Robert

doi:10.1097/00000542-199308000-00020

Cited by 137 publications

(81 citation statements)

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“…Both of these have been validated in studies of functional recovery of sciatic nerve. 38,39 In this study, the functional recovery of denervated muscle in terms of EPT and WRL was minimal, despite the fact that the CMAP amplitude was near normal after 8-12 weeks. This functional recovery was much lower than that reported in other studies (50%), which used muscle force measurement to determine recovery.…”

Section: Discussionmentioning

confidence: 57%

“…Sensory functional recovery was evaluated by measuring nociceptive withdrawal reflex. To evaluate the withdrawal reflex latency (WRL), proposed by Masters et al, 39 a modified version of the hotplate test was used. Briefly, the rat was wrapped in a surgical towel above its midsection and then positioned such that the affected hind paw was in contact with a hot plate set to 56°C.…”

Section: Neurobehavioral Studiesmentioning

confidence: 99%

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Functional Recovery of Completely Denervated Muscle: Implications for Innervation of Tissue-Engineered Muscle

Kang

Olson

Atala

et al. 2012

Tissue Engineering Part A

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Tissue-engineered muscle has been proposed as a solution to repair volumetric muscle defects and to restore muscle function. To achieve functional recovery, engineered muscle tissue requires integration of the host nerve. In this study, we investigated whether denervated muscle, which is analogous to tissue-engineered muscle tissue, can be reinnervated and can recover muscle function using an in vivo model of denervation followed by neurotization. The outcomes of this investigation may provide insights on the ability of tissue-engineered muscle to integrate with the host nerve and acquire normal muscle function. Eighty Lewis rats were classified into three groups: a normal control group (n = 16); a denervated group in which sciatic innervations to the gastrocnemius muscle were disrupted (n = 32); and a transplantation group in which the denervated gastrocnemius was repaired with a common peroneal nerve graft into the muscle (n = 32). Neurofunctional behavior, including extensor postural thrust (EPT), withdrawal reflex latency (WRL), and compound muscle action potential (CMAP), as well as histological evaluations using alpha-bungarotoxin and anti-NF-200 were performed at 2, 4, 8, and 12 weeks (n = 8) after surgery. We found that EPT was improved by transplantation of the nerve grafts, but the EPT values in the transplanted animals at 12 weeks postsurgery were still significantly lower than those measured for the normal control group at 4 weeks (EPT, 155.0 -38.9 vs. 26.3 -13.8 g, p < 0.001; WRL, 2.7 -2.30 vs. 8.3 -5.5 s, p = 0.027). In addition, CMAP latency and amplitude significantly improved with time after surgery in the transplantation group ( p < 0.001, one-way analysis of variance), and at 12 weeks postsurgery, CMAP latency and amplitude were not statistically different from normal control values (latency, 0.9 -0.0 vs. 1.3 -0.7 ms, p = 0.164; amplitude, 30.2 -7.0 vs. 46.4 -26.9 mV, p = 0.184). Histologically, regeneration of neuromuscular junctions was seen in the transplantation group. This study indicates that transplanted nerve tissue is able to regenerate neuromuscular junctions within denervated muscle, and thus the muscle can recover partial function. However, the function of the denervated muscle remains in the subnormal range even at 12 weeks after direct nerve transplantation. These results suggest that tissue-engineered muscle, which is similarly denervated, could be innervated and become functional in vivo if it is properly integrated with the host nerve.

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Section: Discussionmentioning

confidence: 57%

Section: Neurobehavioral Studiesmentioning

confidence: 99%

Functional Recovery of Completely Denervated Muscle: Implications for Innervation of Tissue-Engineered Muscle

Kang

Olson

Atala

et al. 2012

Tissue Engineering Part A

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“…The nociceptive withdrawal reflex (WRL) was adapted from the hotplate test developed by Masters et al (1993) and described elsewhere ( Luis et al, 2007( Luis et al, , 2008a. Normal rats withdraw their paws from the hotplate within 4 s or less ( Hu et al, 1997).…”

Section: Motor Performance and Nociceptive Functionmentioning

confidence: 99%

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

et al. 2012

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Cellular systems implanted into an injured nerve may produce growth factors or extracellular matrix molecules, modulate the inflammatory process and eventually improve nerve regeneration. In the present study, we evaluated the therapeutic value of human umbilical cord matrix MSCs (HMSCs) on rat sciatic nerve after axonotmesis injury associated to Vivosorbs membrane. During HMSCs expansion and differentiation in neuroglial-like cells, the culture medium was collected at 48, 72 and 96 h for nuclear magnetic resonance (NMR) analysis in order to evaluate the metabolic profile. To correlate the HMSCs ability to differentiate and survival capacity in the presence of the Vivosorbs membrane, the [Ca 2þ ]i of undifferentiated HMSCs or neuroglial-differentiated HMSCs was determined by the epifluorescence technique using the Fura-2AM probe. The Vivosorbs membrane proved to be adequate and used as scaffold associated with undiffer-entiated HMSCs or neuroglial-differentiated HMSCs. In vivo testing was carried out in adult rats where a sciatic nerve axonotmesis injury was treated with undifferentiated HMSCs or neuroglial differentiated HMSCs with or without the Vivosorbs membrane. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index (SFI), extensor postural thrust (EPT), and withdrawal reflex latency (WRL).Stereological analysis was carried out on regenerated nerve fibers. In vitro investigation showed the formation of typical neuroglial cells after differentiation, which were positively stained for the typical specific neuroglial markers such as the GFAP, the GAP-43 and NeuN.NMR showed clear evidence that HMSCs expansion is glycolysis-dependent but their differentiation requires the switch of the metabolic profile to oxidative metabolism. In vivo studies showed enhanced recovery of motor and sensory function in animals treated with transplanted undifferentiated and differentiated HMSCs that was accompanied by an increase in myelin sheath. Taken together, HMSC from the umbilical cord Wharton jelly might be useful for improving the clinical outcome after peripheral nerve lesion.

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“…To assess the nociceptive WRL, the hotplate test described by Masters et al [32] was used with some modifications and previously described by the authors [3,9,17,25,31,33] . Also, it is considered by the scientific bibliography that rats without sciatic nerve injury withdraw their paws from the hotplate within 4.3 s or less [9,17,31,33] .…”

Section: % Motor Deficit = [(Nept Eept)/nept] × 100 (1)mentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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Prolonged Regional Nerve Blockade by Controlled Release of Local Anesthetic from a Biodegradable Polymer Matrix

Cited by 137 publications

References 0 publications

Functional Recovery of Completely Denervated Muscle: Implications for Innervation of Tissue-Engineered Muscle

Functional Recovery of Completely Denervated Muscle: Implications for Innervation of Tissue-Engineered Muscle

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

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