Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice

Mustari, Afrina; Nooruzzaman, Mohammed; Miah, Mohammad Alam; Sujan, Khaled Mahmud; Chowdhury, Emdadul Haque

doi:10.1002/vms3.708

Cited by 5 publications

(2 citation statements)

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“…These findings are in line with recent studies by many researchers [ 3 , 12 , 38 ]. Mice given black seed oil had considerably higher serum TT concentrations [ 39 ]. Kaliandra honey had a significant effect on improving TT levels [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Nigella sativa-Floral Honey and Multi-Floral Honey versus Nigella sativa Oil against Testicular Degeneration Rat Model: The Possible Protective Mechanisms

et al. 2023

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The male reproductive function, particularly the testes, and the related hormones are sensitive to various xenobiotics. This work aimed for the first time to assess Nigella sativa floral honey (NS floral honey) and multi-floral honey (M-floral honey) versus Nigella sativa oil (NS oil) against rat testicular degeneration induced with azathioprine (AZA). A total of 40 male Wister rats were assigned into 5 groups: (1) control, (2) 15 mg/kg of AZA, (3) AZA + 1.4 mL/kg of M-floral honey, (4) AZA + 1.4 mL/kg of NS floral honey, and (5) AZA + 500 mg/kg of NA oil. Total testosterone (TT), free testosterone (FT), free androgen index (FAI), gonadotrophins, sex-hormone-binding globulin (SHBG), apoptosis markers, and redox status were assessed to clarify the possible protective mechanisms. Pituitary–testicular axis disruption, apoptosis markers, poor redox status, and sperm quality (count, viability, and motility) were set with AZA. Serum TT, SHBG, and absolute and relative testis weight were significantly restored in the NS oil and NS floral honey groups. Meanwhile, the NS oil group exhibited a significant elevation in FT and FAI. Serum gonadotrophins increased significantly in the NS floral honey (p < 0.01) and M-floral honey and NS oil (p < 0.05) groups. Testicular caspase-3, caspase-9, and nitric oxide showed significant improvement in the NS floral honey and NS oil groups. NS oil supplementation significantly normalized redox status (p < 0.05), whereas NS floral honey improved malondialdehyde and superoxide dismutase activity. Sperm quality exhibited a significant improvement in the NS oil group (p < 0.05). M-floral honey did not show reliable results. Although NS floral honey could protect against testicular damage, it did not upgrade to the level of improvement achieved with NS oil. We claim that further clinical studies are essential for focusing on the quality and quantity of bioactive constituents.

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Section: Discussionmentioning

confidence: 99%

Nigella sativa-Floral Honey and Multi-Floral Honey versus Nigella sativa Oil against Testicular Degeneration Rat Model: The Possible Protective Mechanisms

et al. 2023

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“…3 Infertility is a global health issue that affects approximately 10% -15% of couples trying to conceive and male factor infertility accounts for almost half of the cases. 4 © 2023 the authors. This work is licensed under the Creative Commons Attribution 4.0 International License caused by testicular injury through oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Potential of Thymoquinone on Male Reproductive Functions in Lead Acetate-Induced Reproductive Toxicity in Male Wistar Rats

2023

TJNPR

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Lead is revealed to have unfavorable results on reproductive function, particularly in males. 5 Therefore, this study is one of the concerted efforts in search of alternative ideal curative substances against the deleterious effects of lead-acetate-induced nephrotoxicity particularly in males. Among many medicinal plants, Nigella sativa (Ranunculaceae herbaceous plant), is a medicinally promising herb that has been researched and is well-known for its pharmacological benefits. The seeds of Nigella sativa known as black seed or black cumin are being used as medicine globally having anti-oxidant, anti-fertility, antiinflammatory gastro-protective, anti-tumor, anti-anxiety, and antimicrobial properties. 6,7 Black seed has an active effect on reproductive functions and infertility treatment as it has been demonstrated to cause significant improvements in reproductive parameters such as sperm count, semen qualities, Leydig cells count, follicular development, corpus luteum, and gonadotropic hormones like testosterone and progesterone. 8 Nigella sativa has many different chemical ingredients including thymoquinone (30-48%), flavonoids, anthocyanins, alkaloids, and essential fatty acids, particularly linoleic and oleic acids. 9 Thymoquinone (TQ), the main antioxidant constituent of Nigella sativa seed oil has shown beneficial effects on semen quality and reproductive functions. Several reports have shown that TQ plays a role to increase Leydig cell number and testosterone levels which eventually improved spermatogenesis. 9,10,11 Thymoquinone also exerts marked antioxidant characteristics which could ameliorate the spermatogenesis impairment

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Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice

Mustari

Nooruzzaman

Miah

et al. 2022

Veterinary Medicine & Sci

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Background: Vitamin E and black seed oil are two powerful antioxidants with several health benefits.Objective: The effect of vitamin E and black seed oil on reproductive performance of Swiss albino mice was studied.Methods: A total of 80 (40 male and 40 female) mice of 25-28 days old were randomly divided into four groups viz., A, B, C and D consisting of 10 mice in each group. Mice from the group A served as vehicle control and received normal mice ration whereas mice from the group B, C and D received feed supplemented with either black seed oil (0.5 ml/kg), vitamin E (200 mg/kg) or combination of black seed oil (0.5 ml/kg) and vitamin E (200 mg/kg), respectively daily for 16 weeks. At the end point of the study, blood samples were collected and sera were separated for hormonal analysis. At the same time, mice were sacrificed and testes and ovaries were collected for histomorphological examination. Results:In male mice, the level of testosterone increased significantly in mice receiving black seed oil only, whereas the thyroxin increased significantly in all treated groups when compared to the control mice. Histomorphological examination revealed a significant increase in the diameter of seminiferous tubules in male mice fed with either black seed oil or vitamin E or both. On the other hand, the oestradiol and thyroxin concentration in female mice showed no significant changes in both control and treated groups. However, ovaries of mice fed with black seed oil or vitamin E or both showed an increased number of the follicles of different stages than the control mice. Conclusions:The findings highlighted the promoting action of vitamin E and black seed oil on reproductive functions of mice and that can be used to treat infertility in man and animals.

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Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice

Cited by 5 publications

References 46 publications

Nigella sativa-Floral Honey and Multi-Floral Honey versus Nigella sativa Oil against Testicular Degeneration Rat Model: The Possible Protective Mechanisms

Nigella sativa-Floral Honey and Multi-Floral Honey versus Nigella sativa Oil against Testicular Degeneration Rat Model: The Possible Protective Mechanisms

Ameliorative Potential of Thymoquinone on Male Reproductive Functions in Lead Acetate-Induced Reproductive Toxicity in Male Wistar Rats

Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice

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