Promotion of cardiac differentiation of brown adipose derived stem cells by chitosan hydrogel for repair after myocardial infarction

Wang, Haibin; Shi, Jinxin; Wang, Yan; Yin, Yujing; Wang, Liman; Liu, Jianfeng; Liu, Zhiqiang; Duan, Cuimi; Zhu, Ping; Wang, Changyong

doi:10.1016/j.biomaterials.2014.01.021

Cited by 147 publications

(107 citation statements)

References 36 publications

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“…BADSC delivery with chitosan also achieved enhanced angiogenesis and prevented the negative remodelling of the myocardium. (81) Interestingly in a separate study, a direct comparison of alginate and chitosan β-GP hydrogel using a rat model of MI was carried out, alginate was found to have a greater effect on the retention of hMSCs.…”

Section: Naturally Derived Hydrogels For Cellular Deliverymentioning

confidence: 97%

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

et al. 2016

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Heart failure is a significant clinical issue. It is the cause of enormous healthcare costs worldwide and results in significant morbidity and mortality. Cardiac regenerative therapy has progressed considerably from clinical and preclinical studies delivering simple suspensions of cells, macromolecule, and small molecules to more advanced delivery methods utilizing biomaterial scaffolds as depots for localized targeted delivery to the damaged and ischemic myocardium. Here, regenerative strategies for cardiac tissue engineering with a focus on advanced delivery strategies and the use of multimodal therapeutic strategies are reviewed.

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Section: Naturally Derived Hydrogels For Cellular Deliverymentioning

confidence: 97%

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

et al. 2016

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“…Studies have shown that by combining growth factors with an injectable biomaterial, the biomaterial could serve as a controlled drug-release platform to improve functional outcomes. 21,22 However, co-delivery of BIO and IGF-1 to the injured heart area remains a challenge. Daily injections of both BIO and IGF-1 is a straightforward method that can maintain these agents at appropriate levels; however, this method causes issues, such as toxicity, due to their high doses.…”

Section: Introductionmentioning

confidence: 99%

Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts

Fang¹,

Qiao²,

Liu³

et al. 2015

IJN

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Dedifferentiation and proliferation of endogenous cardiomyocytes in situ can effectively improve cardiac repair following myocardial infarction (MI). 6-Bromoindirubin-3-oxime (BIO) and insulin-like growth factor 1 (IGF-1) are two potent factors that promote cardiomyocyte survival and proliferation. However, their delivery for sustained release in MI-affected areas has proved to be challenging. In the current research, we present a study on the sustained co-delivery of BIO and IGF-1 in a hybrid hydrogel system to simulate endogenous cardiac repair in an MI rat model. Both BIO and IGF-1 were efficiently encapsulated in gelatin nanoparticles, which were later cross-linked with the oxidized alginate to form a novel hybrid hydrogel system. The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function. Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.

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“…13,14 Our previous study found that 1D CNT and 2D graphene-based biomaterials improved the cardiac differentiation of BADSCs. 21 However, the effects of fullerene-C 60 on cardiomyogenic differentiation of BADSCs are unknown.…”

mentioning

confidence: 96%

“…[12][13][14][15] Therapeutic efficacy is limited by the low proliferative rate and cardiac differentiation efficiency in vivo. 16 Previous studies suggested that brown adipose-derived stem cells (BADSCs) are an ideal source for the treatment of MI due to their high cardiomyogenic differentiation potential.…”

mentioning

confidence: 99%

Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression

Hao

Zhou

et al. 2016

IJN

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Zero-dimensional fullerenes can modulate the biological behavior of a variety of cell lines. However, the effects and molecular mechanisms of proliferation and cardiomyogenic differentiation in brown adipose-derived stem cells (BADSCs) are still unclear. In this study, we report the initial biological effects of fullerene-C 60 on BADSCs at different concentrations. Results suggest that fullerene-C 60 has no cytotoxic effects on BADSCs even at a concentration of 100 μg/mL. Fullerene-C 60 improves the MAPK expression level and stem cell survival, proliferation, and cardiomyogenesis. Further, we found that the fullerene-C 60 modulates cardiomyogenic differentiation. Fullerene-C 60 improves the expression of cardiomyocyte-specific proteins (cTnT and α-sarcomeric actinin). At elevated concentration, fullerene-C 60 reduces the incidence of diminished spontaneous cardiac differentiation of BADSCs with time. At the genetic level, fullerene-C 60 (5 μg/mL) also improves the expression of cTnT. In addition, fullerene-C 60 promotes the formation of gap junction among cells. These findings have important implications for clinical application of fullerenes in the treatment of myocardial infarction.

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Promotion of cardiac differentiation of brown adipose derived stem cells by chitosan hydrogel for repair after myocardial infarction

Cited by 147 publications

References 36 publications

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts

Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression

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