Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

Likitpongpipat, Nattriya; Sangmaneedet, Somboon; Klanrit, Poramate; Noisombut, Rajda; Krisanaprakornkit, Suttichai; Chailertvanitkul, Pattama

doi:10.1177/0898756418818891

Cited by 15 publications

(12 citation statements)

References 21 publications

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“…5,6,7,8,9,10,11,12 Animal experiment results indicate that the alcoholic extraction of propolis induces the production of a tubular and better quality dentin than calcium hydroxide groups. 13,14 The present study was designed to compare the effect of propolis and MTA on the biological properties of human dental pulp cells (hDPCs), and lay the experimental foundation for propolis application in pulp therapy in the future.…”

Section: Introductionmentioning

confidence: 99%

Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

2019

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The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 μg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1β and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.

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Section: Introductionmentioning

confidence: 99%

Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

2019

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“…To decrease pain, subcutaneous injection with a non-steroidal anti-inflammatory drug, Carprofen (2 to 4 mg/kg), was given daily for 3 days (14).…”

Section: Direct Pulp Capping Proceduresmentioning

confidence: 99%

“…Rabbits were euthanized at each time interval (14,15) by intravenous injection with a lethal dose (100 mg/kg) of pentobarbital sodium. The rabbits were decapitated, and then the part of maxilla carrying the incisors teeth were dissected.…”

Section: Histological Processingmentioning

confidence: 99%

“…Samples were evaluated using a light microscope (BX41; Olympus, Tokyo, Japan) connected to a high-resolution video camera. The mineralized tissue barrier formation was described according to the modified criteria of Islam, Toida (18), Quantity of Tertiary Dentin Formation was evaluated according to the modified criteria of Yang, Lee (19), morphology of dentinal tubules and extent of inflammation were graded on the report of the modified criteria of Likitpongpipat et al (20).…”

Section: Histological Analysismentioning

confidence: 99%

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Combined Effects of Simvastatin and Calcium Hydroxide Versus Mineral Trioxide Aggregate in Direct Pulp Capping of Rabbit’s Incisors (Histological Study)

elkaramany

elnozahy

elhak

et al. 2023

Alexandria Dental Journal

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INTRODUCTION: Direct pulp capping (DPC) is a procedure to maintain the pulp vitality using a material with specific properties. Mineral trioxide aggregate (MTA) is considered as a gold standard material, but it has some drawbacks. In this study, a combination of simvastatin and calcium hydroxide (SIM-CH) was used as a DPC material as it was proved that simvastatin influenced odontoblast cells differentiation. OBJECTIVE: To compare histologically the effect of the combination of SIM-CH to MTA as a DPC material in rabbits' incisor. MATERIALS AND METHODS: 32 sound maxillary incisor teeth of 16 male New Zealand white rabbits were divided into; group I received MTA, and group II received (SIM-CH) as a DPC material. Class V cavities were done in the upper incisor teeth, then DPC materials were applied. The final restoration was applied. The animals were euthanized after 1, 2 weeks. Routine histological preparation was done, Scoring system was done to evaluate the newly formed dentin. The percentage of the surface area of the newly formed dentin was assisted by histomorphometric analysis. RESULTS: Both groups showed new dentin formation. Group I reported somewhat more continuous layer of tertiary dentin than SIM-CH group. On the other hand, the dentin bridge of the SIM-CH group demonstrated more orderly organized tubules. There was a statistically significant difference regarding the histomorphometric analysis of the percentage of the surface area of the newly formed dentin of group I compared with group II (p< 0.05). CONCLUSION: SIM-CH could be a remarkable alternative DPC material.

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“…Also, multiple teeth can be chosen for any experiment in one rabbit and so the number of animals used in one study is decreased. Rabbits have previously been used in dental studies, but few experiments have examined the impact of various pulp capping products on the promotion of pulp wound healing [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

A comparative histological study of the effect of TheraCal LC and biodentine on direct pulp capping in rabbits: an experimental study

2022

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Objectives This study histologically compared the effect of TheraCal LC and biodentine on direct pulp capping using a rabbit model. Materials and methods A direct pulp capping procedure was performed on 40 mandibular central incisors of 20 healthy, male New Zealand white rabbits. TheraCal LC and biodentine were applied to exposed pulp and 10 randomly selected rabbits were euthanized after the first and second week. Incisors were extracted and prepared for histological processing and examination to check the dentine bridge thickness, continuity, and extent of pulp inflammation. A blinded data analysis was performed, and groups were compared using a Wilcoxon signed-rank test while changes across time within each group were assessed using the Mann–Whitney U test. Results When comparing the dentine bridge thickness, biodentine showed a significantly thicker dentine bridge in the first and second week (mean 28.16 µm, 33.66 µm), while TheraCal LC showed a dentine bridge in the second week only (mean 15.93 µm). Regarding dentine bridge continuity, biodentine showed a significantly better dentine bridge in the first week. Additionally, there was no difference in the second week. Furthermore, there was no statistically significant difference between each of the materials regarding the extent of inflammation. Conclusions Biodentine in vivo showed better results concerning thickness and continuity of the dentine bridge after direct pulp capping in rabbit incisors. Both biodentine and TheraCal LC had a similar inflammatory effect on the pulp. Clinical relevance Biodentine is more successful as a direct pulp capping material compared to TheraCal LC.

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Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

Cited by 15 publications

References 21 publications

Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

Combined Effects of Simvastatin and Calcium Hydroxide Versus Mineral Trioxide Aggregate in Direct Pulp Capping of Rabbit’s Incisors (Histological Study)

A comparative histological study of the effect of TheraCal LC and biodentine on direct pulp capping in rabbits: an experimental study

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