Promotion of enamel caries remineralization by an amelogenin-derived peptide in a rat model

Han, Sili; Fan, Yingying; Zhou, Zhen; Tu, Huanxin; Li, Danxue; Lv, Xuebin; Ding, Lei; Zhang, Linglin

doi:10.1016/j.archoralbio.2016.09.009

Cited by 41 publications

(39 citation statements)

References 34 publications

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“…After flesh removal from around each tooth, the teeth were stained with 0.4% murexide solution for 12 h, then rinsed with distilled water (Han et al, 2017). Maxillary and mandibular molars were ground up along the mesiodistal sagittal plane using the rainbow technique (Super-Snap kit).…”

Section: Caries Scoring Assaymentioning

confidence: 99%

Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated From Traditional Sichuan Pickles

Zhang

Liu

et al. 2020

Front. Microbiol.

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Among cariogenic microbes, Streptococcus mutans is considered a major etiological pathogen of dental caries. Lactobacilli strains have been promoted as possible probiotic agents against S. mutans, although the inhibitory effect of Lactobacilli on caries has not yet been properly addressed. The objective of this study was to screen Lactobacillus strains found in traditional Sichuan pickles and to evaluate their antagonistic properties against S. mutans in vitro and in vivo. In the current study, we analyzed 54 Lactobacillus strains isolated from pickles and found that strain L. plantarum K41 showed the highest inhibitory effect on S. mutans growth as well as on the formation of exopolysaccharides (EPS) and biofilm in vitro. Scanning electron microscopy (SEM) and confocal laser scanning microscope (CLSM) revealed the reduction of both EPS and of the networklike structure in S. mutans biofilm when these bacteria were co-cultured with strain L. plantarum K41. Furthermore, when rats were treated with strain L. plantarum K41, there was a significant reduction in the incidence and severity of dental caries. Due to K41's origin in a high salinity environment, it showed a high tolerance to acids and salts. This may give this strain an advantage in harsh oral conditions. Results showed that L. plantarum K41 isolated from traditional Sichuan pickles effectively inhibited S. mutans biofilm formation and thus possesses a potential inhibitory effect on dental caries in vivo.

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Section: Caries Scoring Assaymentioning

confidence: 99%

Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated From Traditional Sichuan Pickles

Zhang

Liu

et al. 2020

Front. Microbiol.

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“…To promote nucleation as well as render the synthetic peptide highly soluble and stable in water, we added a seven-residue hydrophilic segment (TKREEVD) to a sequence of five Gln-Pro-X repeats (QPYQPVQPHQPMQPQ) and produced another amelogenin-derived peptide, called QP5. As reported in previous studies, it can significantly promote the remineralization of the initial caries in the bovine enamel and the rat enamel without any toxic effects [ 20 , 21 ]. However, it has no effects on limiting the growth of cariogenic bacteria, which is an important contributor to the caries onset and progression.…”

Section: Introductionmentioning

confidence: 68%

Anti-biofilm and remineralization effects of chitosan hydrogel containing amelogenin-derived peptide on initial caries lesions

Ren

Ding

et al. 2018

Regenerative Biomaterials

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In this study, we have designed a more clinically powerful anti-caries treatment by applying the amelogenin-derived peptide QP5 to the antibacterial carrier material chitosan in a hydrogel (CS-QP5 hydrogel), and characterized its effects on the inhibition of a cariogenic biofilm and the promotion of the remineralization of the initial caries lesions. The results indicated that the CS-QP5 hydrogel sustainably inhibited the growth of the Streptococcus mutans biofilm, lactic acid production and the metabolic activity over a prolonged period of time. Moreover, the CS-QP5 hydrogel promoted the remineralization of early enamel lesions, which were indicated by surface micro-hardness (, polarized light microscopy and transverse microradiography. In conclusion, the CS-QP5 hydrogel shows good potential for caries control in the clinic because of its antibacterial effects as well as the remineralization of initial enamel carious lesions even in a biofilm model over a prolonged period of time.

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“…In a more recent study, our group verified the remineralization ability of 8DSS peptide on incident enamel caries and its inhibitory effect on demineralization in vitro (Yang et al, 2014; Yang et al, 2016). Other biomimetic peptides derived from amelogenin have been investigated to study their effects on enamel formation, including a novel peptide designed by our group that included five repeated tandem amelogenin repeats of Gln‐Pro‐X and a hydrophilic tail (Han et al, 2017; Lv et al, 2015). The results of this study and previous findings demonstrated that key sequences of morphogenic proteins could promote biomimetic remineralization of initial carious lesion.…”

Section: Discussionmentioning

confidence: 99%

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

et al. 2020

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With the gradual discovery of functional domains in natural proteins, several biologically inspired peptides have been designed for use as biomaterials for hard tissue regeneration and repair. In this study, we designed a tuftelin-derived peptide (TDP) and tested its effects on hydroxyapatite crystallization and remineralization of initial enamel carious lesions in vitro. Using circular dichroism spectroscopy, we found that TDP contained 36.1% β-sheets and β-turns, which could be influenced by calcium ions. We verified the ability of TDP to crystallize hydroxyapatite using transmission electron microscopy and its ability to bind to the enamel surface and hydroxyapatite using confocal laser scanning microscopy and Langmuir adsorption isotherms (K = 881.56, N = 1.41 × 10 −5). Artificial enamel lesions were generated on human enamel blocks and subjected to a 12-day pH cycling model and were treated with 25 μM TDP, 1 g/L sodium fluoride (NaF), or deionized water. We analyzed the results of remineralization by surface microhardness testing, polarized light microscopy, and transverse microradiography. The TDP group showed significantly higher surface microhardness recovery (49.21 ± 1.66%), shallower lesions (34.89 ± 4.05 μm), and less mineral loss (871.33 ± 81.49 vol%•μm) after pH cycling than the deionized water group (p < .05). There were no significant differences between the TDP and NaF groups. Our experiment indicated that TDP could regulate hydroxyapatite crystallization and promote remineralization of enamel caries in vitro.

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Promotion of enamel caries remineralization by an amelogenin-derived peptide in a rat model

Cited by 41 publications

References 34 publications

Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated From Traditional Sichuan Pickles

Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated From Traditional Sichuan Pickles

Anti-biofilm and remineralization effects of chitosan hydrogel containing amelogenin-derived peptide on initial caries lesions

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

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