Property peculiarities of the atelocollagen–hyaluronan conjugates crosslinked with a short chain di-oxirane compound

Maier, Vasilica; Lefter, Cristina M.; Maier, Stelian Sergiu; Butnaru, Maria; Danu, Maricel; Ibănescu, Constanţa; Popa, Marcel; Desbrières, Jacques

doi:10.1016/j.msec.2014.05.027

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…The strategy builds on the thermal gelation property of agarose as well as capitalizes on the reaction of bisepoxides and multifunctional polyetheramines in water to in situ form polymers capable of temperature-induced phase separation as exemplified by lower critical solution temperature (LCST) characteristics. − The rationale is that by carrying out this reaction in an agarose solution, we can achieve the dual goals of leveraging both the amine characteristics of the polyetheramines for desired cell affinity and LCST-mediated phase separation behavior of the epoxide-amine-derived polymers to create a microstructured morphology in the final gel products. The specific bisepoxide selected for this study is 1,4-butanediol diglycidyl ether (BDDE), a bifunctional epoxide commonly used as a biocompatible cross-linker for collagen, − hyaluronan, − polyethylenimine, silk, and chitosan . The polyetheramine used is one with the trade name Jeffamine T403, a water-soluble triamine featuring a poly(propylene oxide) (PPO) structure.…”

Section: Introductionmentioning

confidence: 99%

Cytophilic Agarose-Epoxide-Amine Cryogels Engineered with Granulated Microstructures

Wang

2023

ACS Appl. Bio Mater.

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Inherent cytophobicity of agarose limits its direct use for the growth of anchorage-dependent cells. Here, we report a simple strategy allowing the development of agarose-based hydrogels entailed with both cytophilicity and microstructured morphology. Through the reaction of water-soluble 1,4-butanediol diglycidyl ether (BDDE) with trifunctional polyetheramine Jeffamine T403 in agarose solution followed by cryogelation of the mixtures, a series of macroporous agarose-epoxide-amine cryogels were prepared readily. Results from fluorescent labeling and energy-dispersive X-ray elemental mapping showed the formation of granulated microstructures in the cryogels. Such features closely correlated to the phase separation of BDDE-T403 polymers within the agarose matrix. Cytophilicity of the microstructured cryogels due to the integrated amine moieties was demonstrated through the adhesion of fibroblasts. Functional enrichment of the cryogels was further highlighted by leveraging the granulates as micro-reservoirs for polyphenol proanthocyanidin to enable antioxidation and protection of fibroblasts from H2O2-induced cytotoxic effect in vitro.

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Section: Introductionmentioning

confidence: 99%

Cytophilic Agarose-Epoxide-Amine Cryogels Engineered with Granulated Microstructures

Wang

2023

ACS Appl. Bio Mater.

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“…An alternative collagen-based biomaterial with extremely low antigenicity is produced by telopeptide removal from natural collagen molecule and it is known as atelocollagen which has been used mainly for e.g. cartilage restoration, bone and connective tissue engineering [19][20][21]. Nevertheless, preliminary works of this group showed that atelocollagen scaffolds also enhanced bone and adipose tissue formation [22,23].…”

Section: Introductionmentioning

confidence: 99%

In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds

Rodrı́guez

Felice

Sánchez

et al. 2016

Materials Science and Engineering: C

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“…However, a different hydrogel system is preferred when distinct types of biomacromolecules are to be crosslinked, especially if the lateral composites involved are also different. 17 In this study, pure pullulan and an HLC-pullulan composition generated by crosslinking with BDDE were investigated. Several types of crosslinkers have been reported, such as glyoxal 14 and dimethyl suberimidate, 15 but the classical 1,4-butanedioldiglycidyl ether (BDDE) is still in use and meet the FDA/EMEA regulations.…”

Section: Introductionmentioning

confidence: 99%

“…The crosslinking sites of BDDE are the free amino and hydroxyl groups, and the crosslinking process is carried out under alkaline conditions. 17 In this study, pure pullulan and an HLC-pullulan composition generated by crosslinking with BDDE were investigated. The mechanical strength, swelling behavior, biodegradation and cell adhesion parameters were evaluated for the two types of hydrogels (PB and PBH hydrogels).…”

Section: Introductionmentioning

confidence: 99%

Novel multifunctional PB and PBH hydrogels as soft filler for tissue engineering

Xue

Liu

et al. 2015

J. Mater. Chem. B

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In this study, we designed multifunctionalized hydrogel scaffolds and injectable particles based on highmolecular-weight (M W ) pullulan and human-like collagen (HLC) crosslinked with 1,4-butanediol diglycidyl ether (BDDE) for combination therapy tissue restoration. The properties of the pullulan/BDDE (PB) and pullulan/BDDE/human-like collagen (PBH) hydrogels were characterized via swelling ratio measurements, mechanical tests, and enzymatic degradation in vitro and via subcutaneous injections in vivo. The results demonstrate that the dry hydrogels completely returned to their original state in deionized water. The elastic modulus of the PBH53 dry hydrogels is higher than that of the other hydrogels after exposure to bending stress and compression stress with a maximum value of 7858.93 MPa. In addition, the in vitro live/dead staining and cell adhesion of the PBH hydrogels exhibited a superior fibroblast morphology without high levels of cell death, which were considerably better than those of PB hydrogels. In vivo, PB and PBH particles with good biocompatibility and anti-biodegradation were successfully prepared via the granulation of wet PB and PBH hydrogels for efficient subcutaneous injection in Kunming mice and New Zealand rabbits. Therefore, the PB and PBH hydrogels were found to be acceptable, safe, soft materials for use in skin restoration, cartilage treatment, and lacrimal dryness therapy. Fig. 3 Cross-sections of the PB and PBH hydrogels before and after degradation by pullulanase, collagenase I and pullulan/collagenase I, as observed by SEM. The first line denotes the cross-section for the four hydrogels (50Â), with a scale bar of 400 mm; the second line represents the cross-section for the four hydrogels at a higher magnification (100Â), with a scale bar of 200 mm; the third line represents the particles for the four hydrogels (100Â), with a scale bar of 200 mm; the fourth line denotes the cross-section for the four hydrogels after degradation by pullulanase (100Â), with a scale bar of 200 mm; the fifth line represents the cross-section for the four hydrogels after degradation by collagenase I (100Â), with a scale bar of 200 mm; and the sixth line denotes the cross-section for the four hydrogels after degradation by pullulanase/collagenase I (250Â), with a scale bar of 100 mm.Fig. 6 Cell adhesion adherent cells on the hydrogels. SEM images of fibroblast attachment on the PB10 hydrogel (A1-A3), PBH10 hydrogel (B1-B3), PB53 hydrogel (C1-C3) and PBH53 hydrogel (D1-D3) at 24 (A1, B1, C1 and D1), 48 (A2, B2, C2 and D2) and 72 h (A3, B3, C3 and D3); the scale bar is 10 mm. Journal of Materials Chemistry B Paper

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Property peculiarities of the atelocollagen–hyaluronan conjugates crosslinked with a short chain di-oxirane compound

Cited by 5 publications

References 32 publications

Cytophilic Agarose-Epoxide-Amine Cryogels Engineered with Granulated Microstructures

Cytophilic Agarose-Epoxide-Amine Cryogels Engineered with Granulated Microstructures

In Vivo evaluation of adipogenic induction in fibrous and honeycomb-structured atelocollagen scaffolds

Novel multifunctional PB and PBH hydrogels as soft filler for tissue engineering

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