Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis ‘Haemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’

Angen, Øystein; Ahrens, Peter; Kuhnert, Peter; Christensen, Henrik; Mutters, Reinier

doi:10.1099/ijs.0.02637-0

Cited by 125 publications

(110 citation statements)

References 29 publications

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“…If the Pasteurellaceae are to be divided into phylogenetically consistent monophyletic groups based on 16S rRNA gene sequences, more than 20 genera will be needed to cover the family . Nomenclatural changes along these lines have been initiated and new genera include Lonepinella (Osawa et al, 1995), Mannheimia (Angen et al, 1999), Phocoenobacter (Foster et al, 2000), Gallibacterium , Histophilus (Angen et al, 2003) and Volucribacter (Christensen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov.

Nørskov‐Lauritsen

Bruun²,

Kilian

2005

International Journal of Systematic and Evolutionary Microbiology

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Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov. The phylogeny of human isolates of Haemophilus species was estimated based on partial sequences of four separate housekeeping genes. The clustering of each set of sequences was in accordance with speciation of the strains with few exceptions: of 108 gene fragments examined, only three appeared to have been subject to recombination events across the species barrier. Housekeeping gene similarity supported previous DNA-DNA hybridization data for the genus rather than the phylogeny inferred from 16S rRNA gene sequence comparison. The similarity of sequences of Haemophilus parainfluenzae with those of Haemophilus influenzae suggested preservation of the former species in the genus Haemophilus. Three strains representing a novel taxon were unique with respect to the four investigated gene loci. 16S rRNA gene sequence analysis suggested that this taxon belonged to the Parainfluenzae cluster. DNA-DNA hybridization data supported this generic placement. Nine strains of the novel taxon were available for analysis. They were distinct from representatives of all current species of the genus Haemophilus by conventional phenotypic characterization. Genotypic and phenotypic data show that the strains merit recognition as a novel species of Haemophilus. The name Haemophilus pittmaniae sp. nov. is proposed, with HK 85 T (=CCUG 48703 T =NCTC 13334 T ) as the type strain.

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Section: Introductionmentioning

confidence: 99%

Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov.

Nørskov‐Lauritsen

Bruun²,

Kilian

2005

International Journal of Systematic and Evolutionary Microbiology

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“…Analysis of the rpoB gene encoding the beta-subunit of RNA polymerase has greatly supported the identification and the elaboration of genetic relationships in several groups of bacteria (Mollet et al, 1997;Kim et al, 2003;Korczak et al, 2004;Christensen et al, 2004;Drancourt et al, 2004). Recently, the rpoB gene has also been used to support the description of novel taxa (Angen et al, 2003;Kuhnert et al, 2004).In this study, we used a universal amplification and sequencing approach for the rpoB gene to investigate genetic relatedness within the genus Campylobacter.Sequence comparison analysis was used to select the primers CamrpoB-L, 59-CCAATTTATGGATCAAAC-39, and RpoB-R, 59-GTTGCATGTTNGNACCCAT-39, which enabled the amplification of a 524 bp fragment from the rpoB gene of most Campylobacter species. The PCR product corresponded to region 1536-2059 bp of the Escherichia coli rpoB gene.…”

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confidence: 99%

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confidence: 99%

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Korczak

Stieber

Emler

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.The genus Campylobacter was first proposed by M. Sebald and M. Véron in 1963 and included only the type species Campylobacter fetus and Campylobacter bubulus, now known as Campylobacter sputorum (Sebald & Véron, 1963). These taxa had formerly been classified as Vibrio species. In 1973, M. Véron and R. Chatelain included several misclassified Vibrio in the distinct Campylobacter genus based on serological, biochemical and DNA-DNA hybridization investigations (On, 2001). Since then, the taxonomy of the genus has changed dramatically. At present, it comprises 17 species with validly published names and six recognized subspecies (On, 2001;Foster et al., 2004). In addition, strains belonging to C. sputorum are divided into three biovars, sputorum, fecalis and paraureolyticus, on the basis of their ability to produce catalase or urease Vandamme & On, 2001).In general, members of the genus Campylobacter colonize the mucosal surfaces of the intestinal tract, oral cavity or urogenital tract of healthy, as well as diseased, humans and animals, especially birds. Several species may act as pathogens, causing disease in both human and animal hosts. Twelve of the 17 Campylobacter species are associated with human diseases. Campylobacter jejuni and Campylobacter coli are particularly frequent causative agents of human bacterial intestinal disorders worldwide (Skirrow, 1994). There have also been reported cases of diarrhoea in man caused by Campylobacter upsaliensis and Campylobacter lari, but the frequency of these infections is very low (Bourke et al., 1998;Van Doorn et al., 1998). Occasionally, Campylobacter species are implicated as causative agents of pericard...

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“…somni was a recently described bacterial species as a combination of "Haemophilus somnus", "Haemophilus agni" and "Histophilus ovis" [1]. The organisms can be pathogens responsible for substantial economic losses in cattle and sheep industries due to a variety of diseases, such as thrombotic meningoencephalitis, pneumonia, myocarditis, and reproductive disorders in cattle [17,18,27] as well as mastitis, septicemia, and reproductive disorders in sheep [4,18,38].…”

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confidence: 99%

“…Such a method reflecting on the boundary can be a useful tool for understanding the epidemiology of the organisms. Based on the phylogenies of the 16S rDNAs, H. somni strains are clearly distinguished from the related taxa in the family Pasteurellaceae [1]. In addition to the use of 16S rDNA sequencing for taxonomic purposes, subtle sequence differences in the 16S rDNA sequence were recently reported to be useful for species identification [34,39] and for subtyping and identifying hyper-virulent bacterial clones [5,31,33].…”

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confidence: 99%

Differentiation between Bovine and Ovine Strains of Histophilus somni Based on the Sequences of 16S rDNA and rpoB Gene

Tanaka

Hoshinoo

Hoshino

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Nucleotide sequences of 16S rDNA and rpoB gene of 25 bovine and 6 ovine Histophilus somni strains were determined to detect subtle differences between the host animal species. The 1465 nucleotide residues of the 16S rDNA exhibited levels of sequence similarities of 99.4% or more. The high sequence similarity of the 16S rDNA of recently described species H. somni was confirmed in the 31 strains from cattle and sheep. These results suggested that the intra-specific diversity of 16S rDNA was limited in bovine and ovine strains of H. somni. The specific association of strains was also observed in the 311 bp region of rpoB gene which sequence similarities were 98.6% or more. However, the phylogenetic tree analysis of the rpoB gene showed that the ovine strains appeared to form a subgroup recovered in 70% of the bootstrap trees. In the 311 bp region of the ovine strains, a HincII restriction endonuclease site was detected. The PCR-amplified rpoB DNA of 46 bovine and 20 ovine H. somni strains were examined for the digestion with HincII. As the results, 17 strains of ovine strains were cleaved by the enzyme but none of the bovine strains appeared to possess the restriction site. The restriction enzyme analysis of rpoB gene may be useful to differentiate ovine strains from bovine strains of H. somni. H. somni was a recently described bacterial species as a combination of "Haemophilus somnus", "Haemophilus agni" and "Histophilus ovis" [1]. The organisms can be pathogens responsible for substantial economic losses in cattle and sheep industries due to a variety of diseases, such as thrombotic meningoencephalitis, pneumonia, myocarditis, and reproductive disorders in cattle [17,18,27] as well as mastitis, septicemia, and reproductive disorders in sheep [4,18,38]. H. somni is also distributed in nasopharyngeal tracts and reproductive organs of healthy animals [8-11, 19, 20, 29, 41, 45]. Based on the profiles of outer membrane protein, a restriction endonuclease analysis, PCR-based fingerprinting analyses and a DNA reassociation analysis, it has been suggested that there are host-specific subgroups in the organisms [3,23,46,47]. Up to the date, however, no simple mean to differentiate between bovine and ovine strains of H. somni has been available. Such a method reflecting on the boundary can be a useful tool for understanding the epidemiology of the organisms. Based on the phylogenies of the 16S rDNAs, H. somni strains are clearly distinguished from the related taxa in the family Pasteurellaceae [1]. In addition to the use of 16S rDNA sequencing for taxonomic purposes, subtle sequence differences in the 16S rDNA sequence were recently reported to be useful for species identification [34,39] and for subtyping and identifying hyper-virulent bacterial clones [5,31,33]. For the same purposes, the rpoB gene encoding the β-subunit of RNA polymerase has been studied for many bacterial species, such as Bacillus [24] although sequence stretches or cluster groups corresponding to the host-specific subgroups were not clear d...

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Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis ‘Haemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’

Cited by 125 publications

References 29 publications

Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov.

Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov.

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Differentiation between Bovine and Ovine Strains of Histophilus somni Based on the Sequences of 16S rDNA and rpoB Gene

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