Propriétés antimicrobiennes et toxicité par détermination de la DL50 de l’huile essentielle de Prangos asperula Boissier

Abstract

Cette recherche concerne l'étude des propriétés antimicrobiennes de l'huile essentielle d'une plante sauvage, Prangos asperula Boissier, et de la détermination du seuil de sa toxicité.Les propriétés antimicrobiennes ont été étudiées sur cinq germes :-Streptococcus fecalis, Staphylococcus aureus (bactéries gram +) ; -Salmonella typhi, Escherichia coli (bactéries gram -) ; -Candida albicans (levure). La toxicité de cette huile essentielle a été évaluée à partir de plusieurs expérimentations sur des souris testées au laboratoire.Les essais pour une concentration déterminée ont été effectués sur une série de cinq souris. Les concentrations d'huile essentielle appliquées varient de 20 à 160 mL/mL. Après injection de la solution d'huile essentielle, des observations sur le comportement des souris ont été notées. La dose létale 50 (DL 50 ) a été déduite à partir de la droite tracée décrivant la mortalité des souris en fonction de la concentration de la solution injectée. La DL 50 trouvée est de 1,05 mL/g. Abstract:This research concerns the antimicrobial properties of an essential oil of a wild plant, Prangos asperula Boissier, and the determination of its toxicity threshold.Antimicrobial properties were tested against five germs: -Streptococcus fecalis, Staphylococcus aureus (gram positive bacteria); -Salmonella typhi, Escherichia coli (gram negative bacteria); -Candida albicans (yeast). The toxic effect of this essential oil was tested on mice in a laboratory. Different concentrations of the essential oil were used, ranging from 20 to 160 ml/ml. Each was tested on a series of five mice. After essential oil solution injection, the behavior of the mice was documented. The lethal dose (LD 50 ) was deduced from the straight line describing the death rate in relation to the concentration of injected essential oil solution. The LD 50 found was about 1,05 mL/g.

Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive

“…Serial doses of 25, 50, 75, 100, 150 and 350 mg/mL of PSEO diluted in Tween 80 (1% v/v), as described by Hilan et al (2009), were administrated by intraperitoneal injection to 6 groups in a proportion of 0.01 mL/g of mice body weight. One group, served as vehicle control, received…”

“…2). The LD 50 is crucial for the prediction of the symptomatology of poisoning after acute overdosing in humans (Hilan et al, 2009). It was demonstrated that intraperitoneal LD 50 values for anethole, major compound in PSEO, were ranged between 650 and 1410 mg/kg in mice.…”

Evaluation of Antioxidant, Antibacterial, Antalgic and Anti-Inflammatory Properties of Crataegus Azarolus

“…Five groups were formed, each containing six mice. The first group received physiological saline solution orally (control -), and the other groups were treated with various concentrations of the methanolic extract (600, 1000, 2000, 4000 mg/kg), which were then observed for 72 hrs for toxicity symptoms and mortality rate 17 .…”

Prangos platychlaena: State of the Art from Ethnopharmacology, Phytochemical, Toxicological and Pharmacological Perspective

“…There are also other biological factors, including death, and the starting date, time and degree of survival recovery are important in toxicity evaluation (Chinedu, Arome, & Ameh, 2013). According to (Hilan, Bouaoun, Aoun, Sfeir, & Garabeth, 2009), the value of LD50 in relation to the concentration of injected essential oil of P. asperula, was analyzed from the straight line defining the death rate, which was approximately 1,05 µL/g. While, the LD50 of the Osthole (7-methoxy-8-(3-methyl-2butenyl)-2H-1-benzopyran-2-one) was 710 mg/kg, which isolated from the root of Prangos ferulacea (Yalda Shokoohinia et al, 2017).…”