Prospective isolation of resident adult human mesenchymal stem cell population from multiple organs

Mabuchi, Yo; Matsuzaki, Yuji

doi:10.1007/s12185-015-1921-y

Cited by 33 publications

(25 citation statements)

References 81 publications

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“…MSCs are multipotent stem cells capable of self-renewal and differentiation into mesodermal lineages such as chondrocytes, adipocytes and osteocytes [25] . These cells have been identified in multiple tissues such as BM, umbilical cord blood, amniotic fluid, adipose tissue and dental pulp [26] . MSCs are considered a novel modality of therapy for a wide variety of degenerative and immunological disorders.…”

Section: Discussionmentioning

confidence: 99%

Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage

Ogata

Mabuchi

Shinoda

et al. 2018

Regenerative Therapy

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IntroductionThe anterior cruciate ligament (ACL) consists of various components, such as collagen, elastin fibres, and fibroblasts. Because ACL has a poor regenerative ability, ACL reconstruction need require the use of autologous tendons. In recent years, tissue-resident stem cells have been studied to promote ACL regeneration as an alternatively method. However, the existence of stem cells in ligaments has not been clearly defined. Here, we prospectively isolated stem cells from ACLs and characterized their properties.MethodsACLs from 11 donors and bone marrows (BM) from 8 donors were obtained with total knee arthroplasty. We used flow cytometry to screen the cell surface markers on ACL cells. Frozen sections were prepared from patient ACL tissues and stained with specific antibodies. Cultured ACL-derived and BM-derived cells at passage 3 were differentiated into adipocytes, osteoblasts and tendon/ligament cells.ResultsACL-derived mesenchymal stem/stromal cells (ACL-MSCs) expressed high levels of CD73 and CD90. Immunohistochemical analyses revealed that ACL-MSCs were located on the inner surface of ACL sinusoids. Furthermore, the expression of cell surface antigens was clearly different between ACL-MSCs and bone marrow (BM)-derived MSCs (BM-MSCs) at the time of isolation, but the two cell populations became indistinguishable after long-term culture. Interestingly, ACL-MSCs are markedly different from BM-MSCs in their differentiation ability and have a high propensity to differentiate into ligament-committed cells.ConclusionsOur findings suggest that ACL-MSCs express CD90 and CD73 markers, and their differentiation capacity is maintained even through culture. The cell population having tissue-specific properties is an important research target for investigating the ligament therapies.

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Section: Discussionmentioning

confidence: 99%

Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage

Ogata

Mabuchi

Shinoda

et al. 2018

Regenerative Therapy

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“…Beside the matter of quantity, in the last decades, MSCs were better characterized from several points of view. Although MSCs still lack a single specific surface marker, panels of markers guiding their identification have been proposed . Other features have been described and are now shared by the scientific community, such as genomic, epigenetic, secretomic, and proteomic profiling .…”

Section: Learning From More Than 50 Years Of Msc Investigationsmentioning

confidence: 99%

Challenges in Clinical Development of Mesenchymal Stromal/Stem Cells: Concise Review

Mastrolia

Foppiani

Murgia

et al. 2019

Stem Cells Translational Medicine

235

182

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Summary Identified 50 years ago, mesenchymal stromal/stem cells (MSCs) immediately generated a substantial interest among the scientific community because of their differentiation plasticity and hematopoietic supportive function. Early investigations provided evidence of a relatively low engraftment rate and a transient benefit for challenging congenital and acquired diseases. The reasons for these poor therapeutic benefits forced the entire field to reconsider MSC mechanisms of action together with their ex vivo manipulation procedures. This phase resulted in advances in MSCs processing and the hypothesis that MSC‐tissue supportive functions may be prevailing their differentiation plasticity, broadening the spectrum of MSCs therapeutic potential far beyond their lineage‐restricted commitments. Consequently, an increasing number of studies have been conducted for a variety of clinical indications, revealing additional challenges and suggesting that MSCs are still lagging behind for a solid clinical translation. For this reason, our aim was to dissect the current challenges in the development of still promising cell types that, after more than half a century, still need to reach their maturity. Stem Cells Translational Medicine 2019;8:1135–1148

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“…MSCs reside in the BM 2 and various other tissues such as the umbilical cord 3 , synovium 4–6 , muscle 7 , fat 8 , and dental pulp 9 . MSCs can self-renew, form colonies, and differentiate into several mesenchymal lineages including osteoblasts, chondrocytes, and adipocytes 10 .…”

Section: Introductionmentioning

confidence: 99%

Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation

Suto

Mabuchi

Suzuki

et al. 2017

Sci Rep

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Mesenchymal stem/stromal cells (MSCs), which reside in the bone marrow (BM) and various other tissues, can self-renew and differentiate into mesenchymal lineages. Many groups have harvested rat MSCs (rMSCs) from rat BM (rBM) by using a flush-out procedure and have evaluated surface marker expression after long-term culture. However, MSCs gradually differentiate during expansion and exhibit altered proliferation rates, morphological features and functions in vitro. Variations in MSC isolation methods may alter the effectiveness of therapeutic applications. Here, on the basis of CD29 (Itgb1) and CD54 (Icam1) expression, we prospectively isolated a population with a high colony-forming ability and multi-lineage potential from the rBM, and we demonstrated that most of these cells expressed CD73. Successful engraftment of rMSCs was achieved by using a fluorescence-conjugated anti-CD73 antibody. In humans and mice, MSCs were also purified by CD73, thus suggesting that CD73 may serve as a universal marker for prospective isolation of MSCs. Our results may facilitate investigations of MSC properties and function.

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Prospective isolation of resident adult human mesenchymal stem cell population from multiple organs

Cited by 33 publications

References 81 publications

Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage

Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage

Challenges in Clinical Development of Mesenchymal Stromal/Stem Cells: Concise Review

Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation

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