Prospective trial of a 2940 nm Er:YAG laser for the treatment of meibomian gland dysfunction

Fu, Yana; Xiang, Huan; Hu, Renjian; Huang, Xiaodan; Lin, Lin; Jin, Xiuming

doi:10.1007/s00417-021-05170-8

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…Clinical assessments were performed sequentially as follows: SPEED questionnaire, fluorescein tear break-up time (TBUT), corneal fluorescein staining (CFS) (Song et al, 2013), lid abnormality (LAM) (Fu et al, 2021), meibum expressibility, and meibography. We assessed the meibum expressibility of 15 glands in each lower eyelid (0-45) (Lane et al, 2012).…”

Section: Participant Examinationmentioning

confidence: 99%

Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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PurposeTo compare the ocular surface and meibum microbial communities of humans with Demodex Blepharitis (DB) and healthy controls.MethodsConjunctival sac and meibum samples from 25 DB patients and 11 healthy controls were analyzed using metagenomic next-generation sequencing (mNGS).ResultsThe alpha-diversity of the conjunctival sac microbiome of the DB group (observed, Chao1, ACE) was lower than that of the control group, whereas all meibum diversity indicators were similar. In conjunctival samples, the relative abundance (RA) of the phylum Proteobacteria was significantly higher (p=0.023), and the RA of both phyla Actinobacteria and Firmicutes was significantly lower (p=0.002, 0.025, respectively) in the DB group than that in the control group. In meibum samples, the RA of the phyla Proteobacteria and Actinobacteria were similar, whereas that of the phylum Firmicutes was significantly lower in the DB group (p=0.019) than that in the control group. Linear discriminant analysis with effect size measurement of the conjunctival and meibum microbiomes showed that Sphingobium sp. YG1 and Acinetobacter guillouiae were enriched in the DB group. Sphingobium sp. YG1, Acinetobacter guillouiae and Pseudomonas putida in the DB group were related to more severe ocular surface clinical parameters. Discriminative genera’s principal coordinate analysis separated all control and DB microbiomes into two distinct clusters.ConclusionsProteobacteria’s increased prevalence may indicate ocular microbial community instability. The species Sphingobium sp. YG1 and Acinetobacter guillouiae are potentially pathogenic bacterial biomarkers in DB. Demodex infection mainly affects the ocular surface microbiome rather than penetrating deeper into the meibomian gland.

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Section: Participant Examinationmentioning

confidence: 99%

Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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“…A total OSDI scores ranged from 0 to 100. The results were interpreted as follows: normal (scores 0–12) and dry eye (scores 13–100) 18 .This study used Chinese version and validated in previous studies 19 , 20 .…”

Section: Methodsmentioning

confidence: 99%

Rear 4-min Schirmer test, a modified indicator of Schirmer test in diagnosing dry eye

Wang

Fan

et al. 2022

Sci Rep

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This study aims to investigate the reliability and efficacy of rear 4-min Schirmer test, as a supplement indicator, in assessing tear secretion and diagnosing dry eye. 180 participants were enrolled in this study. Schirmer test I without anaesthesia was performed once on both eyes to determine the value of normal Schirmer test. The values of tear secretion were recorded at each minute. Other examinations included the following: the ocular surface disease index (OSDI), the standard patient evaluation of eye dryness (SPEED), fluorescein stain, tear film break-up time (BUT), and Meibomian gland (MG) secretion grading. The participants were divided into dry eye (DE) group and non-dry eye (ND) group. The values of the 2-min Schirmer test, rear 3-min Schirmer test, rear 4-min Schirmer test, and 5-min Schirmer test were 5.36 ± 4.63, 5.57 ± 2.11, 7.21 ± 4.13, and 10.93 ± 6.30, respectively, in the DE group. These indicators were 8.25 ± 6.80, 2.73 ± 2.31, 7.36 ± 3.42, and 11.84 ± 6.16, respectively, in the ND group. The rear 4-min Schirmer test had a significant correlation with OSDI and SPEED in the DE group (r = − 0.242/ − 0.183) and in the ND group (r = − 0.316/ − 0.373). Meanwhile, the rear 4-min Schirmer test had a stronger connection with fBUT (r = 0.159) and MG secretion (r = − 0.162) in the DE group and also had higher accuracy in diagnosing severe DE and borderline DE. In conclusion, the rear 4-min Schirmer test may be a supplement indicator in assessing tear secretion and diagnosing DE.

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Prospective trial of a 2940 nm Er:YAG laser for the treatment of meibomian gland dysfunction

Cited by 2 publications

References 29 publications

Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients

Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients

Rear 4-min Schirmer test, a modified indicator of Schirmer test in diagnosing dry eye

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