Prostaglandins E₂ and F_2α Enhance Differentiation of Cementoblastic Cells

Abstract

We conclude that PGE(2) and PGF(2alpha) exert an anabolic effect on OCCM mineralization through activation of PKC signaling.

Prostaglandin F2α-Induced Interleukin-8 Production in Human Dental Pulp Cells Is Associated With MEK/ERK Signaling

“…PGF 2a has been shown to induce the differentiation of cementoblasts and stimulate the matrix metalloproteinase activity as well as IL-6 production of gingival fibroblasts (10,11,31). In this study, exposure to PGF 2a stimulates IL-8 production of pulp cells.…”

Ultrasound Stimulation Induces PGE2 Synthesis Promoting Cementoblastic Differentiation Through EP2/EP4 Receptor Pathway

“…PGE 2 is one of the most important signaling molecules produced by the cells. Although PGE 2 , has been related to bone resorption during the early inflammatory phase, it also may contribute to attenuate the inflammatory reaction and induce healing process at a later stage, because many studies have also reported an anabolic effect of this prostanoid in bone and also in periodontal tissues (Keller et al 1993;Marks and Miller 1994;RamiresYanes et al 2005;Camargo et al 2005). PGE 2 action is mediated through four subtype receptors belonging to the G-protein-couple receptor family (EP1, EP2, EP3 and EP4).…”

Regulation of Nur77 gene expression by prostanoids in cementoblastic cells

“…Locally delivered PGE 1 increases the formation of alveolar bone, PDL and cementum in adult dogs (18). IL-1 inhibits mineralization by an immortalized cementoblastic cell line (OC-CM cells) while PGE 2 and PGF 2α have an anabolic effect on mineral nodule formation by OC-CM cells (19). Fluprostenol (flup), a synthetic prostanoid agent with a molecular structure similar to PGF 2α , exerts a well-defined, dose-dependent anabolic effect on mineralization by OC-CM cells (19).…”

“…IL-1 inhibits mineralization by an immortalized cementoblastic cell line (OC-CM cells) while PGE 2 and PGF 2α have an anabolic effect on mineral nodule formation by OC-CM cells (19). Fluprostenol (flup), a synthetic prostanoid agent with a molecular structure similar to PGF 2α , exerts a well-defined, dose-dependent anabolic effect on mineralization by OC-CM cells (19). While this anabolic effect appears to be, in part, mediated by activation of the protein kinase C (PKC) signaling pathway (19), the exact molecular mechanism of the prostanoid-induced mineralization by OC-CM cells is not well understood.…”

“…The EP1 and FP receptors preferentially activate the PKC signaling pathway and calcium signaling. EP2 and EP4 receptors activate, while EP3 receptors inhibit, the cyclic AMP-protein kinase A (PKA) signaling pathway (19, 21). …”