Protection of chickens from fowl cholera by vaccination with recombinant adhesive protein of Pasteurella multocida

Sthitmatee, Nattawooti; Numee, Sureerat; Kawamoto, Eiichi; Sasaki, Hiraku; Yamashita, Kaoru; Takahashi, Naoyuki; Kataoka, Yuki; Sawada, Takuo

doi:10.1016/j.vaccine.2008.02.051

Cited by 33 publications

(44 citation statements)

References 24 publications

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“…Although this protein was obtained from a capsular extract and appeared at the same size as the PlpB protein, they were dis- Note: This sequence block (nucleotide 598-825) should come after nucleotide 538. similar proteins. As claimed by Sthitmatee and colleagues this protein has N-terminal sequence similar to the major outer membrane (OmpH) which was reported earlier by Luo et al [11,5]. In conclusion, our data indicate that PlpB protein may not be an appropriate target as a candidate subunit vaccine for P. multocida infection.…”

Section: Discussionsupporting

confidence: 57%

“…The purified 39 kDa capsular protein showed high protection against P. multocida serotype A:1 and A:3 in mice and the recombinant protein also protected chicken from challenge by both serotypes [2,11]. Although this protein was obtained from a capsular extract and appeared at the same size as the PlpB protein, they were dis- Note: This sequence block (nucleotide 598-825) should come after nucleotide 538. similar proteins.…”

Section: Discussionmentioning

confidence: 99%

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Expression and Immunoprotective Property of a 39-kDa PlpB Protein of Pasteurella multocida

Chomnawang

Nabnuengsap

Kittiworakarn

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Pasteurella multocida is a Gram-negative bacterium known to infect a wide range of domestic and wild animals. The increasing incidence of P. multocida isolated from cases of fowl cholera and hemorrhagic septicemia has led to a renewed interest in this pathogen as well as in the development of vaccines. In this study, PCR primers were designed to amplify the fragment of plpB gene from P. multocida FC-Pakchong (A:1). The purified PCR product of plpB gene consisting of 831 base pairs was inserted into the pGEX-5X-1 plasmid, which expressed the GST protein, and then transformed into E. coli. The purified fusion GST-PlpB protein showed a major band of about 63 kDa on SDS-PAGE gel. After enzyme cleavage, the recombinant PlpB protein appeared at the estimated size of 36 kDa. The recombinant GST-PlpB protein was tested for the toxicity in vivo. The results showed no toxicity in mice at the highest tested concentration of the protein. Moreover, the immunoprotective property of the recombinant GST-PlpB protein was determined in mice after subcutaneous immunization and challenge with an approximate dose of 50-100 LD 50 of P. multocida serotype A:1 and A:3,4. It was demonstrated that this subunit vaccine provided 20-30% survival rate after subcutaneous immunization and challenge with an approximate dose of 50-100 LD 50 of P. multocida serotype A:1 and A:3,4 whereas all of the non-immunized mice died from P. multocida infection. In conclusion, our data indicated that the PlpB protein may not be an appropriate target as a candidate subunit vaccine for P. multocida infection.KEY WORDS: 39-kDa outer membrane associated protein, fowl cholera, Pasteurella multocida, PlpB, subunit vaccine.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Expression and Immunoprotective Property of a 39-kDa PlpB Protein of Pasteurella multocida

Chomnawang

Nabnuengsap

Kittiworakarn

et al. 2009

The Journal of Veterinary Medical Science

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“…Putative adhesins that enhance colonization by P. multocida have been reported (34, [433][434][435][436][437][438][439][440][441][442]. OmpA is an outer membrane protein (OMP) that serves as an adhesin by binding to host extracellular matrix proteins, such as fibronectin (437,438,443).…”

Section: Survival In the Host Environmentmentioning

confidence: 99%

“…For example, antisera against the outer membrane protein Oma87 protected mice against a lethaldose challenge of P. multocida (447). Vaccination with recombinant adhesion protein Cp39 from P. multocida strain P-1059 protected chickens from challenge with strain P-1059 (serotype A:3) and strain X-73 (serotype A:1) (436,448). OmpH-specific antibodies were more effective than OmpA-specific antibodies in curbing P. multocida growth in mice, presumably by enhancing PMN phagocytosis (562).…”

Section: Vaccinationmentioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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SUMMARY In a world where most emerging and reemerging infectious diseases are zoonotic in nature and our contacts with both domestic and wild animals abound, there is growing awareness of the potential for human acquisition of animal diseases. Like other Pasteurellaceae , Pasteurella species are highly prevalent among animal populations, where they are often found as part of the normal microbiota of the oral, nasopharyngeal, and upper respiratory tracts. Many Pasteurella species are opportunistic pathogens that can cause endemic disease and are associated increasingly with epizootic outbreaks. Zoonotic transmission to humans usually occurs through animal bites or contact with nasal secretions, with P. multocida being the most prevalent isolate observed in human infections. Here we review recent comparative genomics and molecular pathogenesis studies that have advanced our understanding of the multiple virulence mechanisms employed by Pasteurella species to establish acute and chronic infections. We also summarize efforts being explored to enhance our ability to rapidly and accurately identify and distinguish among clinical isolates and to control pasteurellosis by improved development of new vaccines and treatment regimens.

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“…Whole-cell lysates of strains P-1059 and PBA322 and purified recombinant OmpH (rOmpH protein) [26] of strain P-1059 were analyzed on SDS-PAGE slab gel. Strain PBA322 showed small amounts of OmpH on SDS-PAGE when compared to the parental strain P-1059 (Fig.…”

Section: Sds-page and Immunoblottingmentioning

confidence: 99%

Inhibition of Capsular Protein Synthesis of Pasteurella multocida Strain P-1059

Sthitmatee

Kataoka

Sawada

2011

The Journal of Veterinary Medical Science

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ABSTRACT. A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens.KEY WORDS: antisense RNA, fowl cholera, noncapsulated strain, Pasteurella multocida.

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Protection of chickens from fowl cholera by vaccination with recombinant adhesive protein of Pasteurella multocida

Cited by 33 publications

References 24 publications

Expression and Immunoprotective Property of a 39-kDa PlpB Protein of Pasteurella multocida

Expression and Immunoprotective Property of a 39-kDa PlpB Protein of Pasteurella multocida

Pasteurella multocida: from Zoonosis to Cellular Microbiology

Inhibition of Capsular Protein Synthesis of Pasteurella multocida Strain P-1059

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