Protection of DNA and erythrocytes from free radical induced oxidative damage by black gram (Vigna mungo L.) husk extract

Girish, Talakatta K; Padmaraju, Vasudevaraju; Rao, U.J.S. Prasada

doi:10.1016/j.fct.2012.01.043

Cited by 31 publications

(32 citation statements)

References 31 publications

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“…Then, HL in-hibition was assessed by measuring the hemoglobin concentration in the reaction [12]. In this study, PFNE proficiently up-regulated erythrocyte membrane stability and, thus, reduced HL.…”

Section: Hl Inhibition Capacity In Rat Erythrocytesmentioning

confidence: 98%

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Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Islam

Streck²,

Paz³

et al. 2016

Int Arch Med

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The study prepared a nanoemulsion with a diterpenoid isoprenoid alcohol called phytol (PYT) and subsequently tested it for antioxidant capacity. For this, PYT-loaded nanoemulsion was prepared by phase inversion method and both PYT-containing nanoemulsion (PNE) and PYT-free nanoemulsion (PFNE) (2-16 µM) were tested for antiradical activity (DPPH•: 1,1-dipheny-picrylhydrazyl radical; ABTS•+: azino-bisethylbenzthiazoline-sulfonic acid; •OH: hydroxyl radical scavenging; NO•: nitrite oxide radical), lipid peroxidation (LP), reduction potential (RP), and inhibition of hemolysis (HL) in rat erythrocytes in comparison with an α-tocopherol analogue (Trolox -TRO -positive control). In addition, an in vivo test was performed with wildtype and deficient Saccharomyces cerevisiae strains using hydrogen peroxide (H 2 O 2 ) as a stressor. Results suggest that PNE exhibited higher antioxidant than the PFNE. Increasing doses reveled antioxidant capacity in a dose-dependent manner. In the S. cerevisiae study, both PFNEand PNE-treated groups exhibited decreased rates of survival with the highest doses, whichever in the presence of stressor increased the survival rates, which indicates antioxidative defense capacity of PYT. In this occasion, PNE exhibited prominent antioxidative defense in the presence of stressor rather than PFNE. In conclusion, PYT exhibited potential antioxidant activity but at high concentration it was toxic to the yeast cells. The production of PYT-nanoemulsions may be relevant to the pharmaceutical sciences.

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Section: Hl Inhibition Capacity In Rat Erythrocytesmentioning

confidence: 98%

“…The reaction mixture was incubated at 37 °C for 30 minutes and immediately centrifuged at 2,500 rpm for 3 minutes. Next, 0.2 mL of the supernatant was mixed with 2.8 mL PBS (pH 7.4) and the absorbance was measured at 475 nm [12]. For the NC, 0.2 mL of the vehicle was added.…”

Section: Hl Inhibition Capacity In Rat Erythrocytesmentioning

confidence: 99%

Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Islam

Streck²,

Paz³

et al. 2016

Int Arch Med

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“…This test is an adjustment of the earlier methods described by Ruch et al 8 and Girish et al 9 . In this context, blood was collected from a healthy man through venous puncture and immediately reconstituted 10% RBC suspension (HRBC) with the phosphate buffer saline (PBS, pH 7.4).…”

Section: H 2 O 2 -Induced Hemolysis (Hl) Testmentioning

confidence: 99%

A Possible Pro-Oxidative Triggered Anti-Atherothrombotic Effect of Dysophylla Auricularia Hydro-Alcoholic Extract

Islam¹

2016

IJPSR

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Oxidative stress can trigger inflammation and atherosclerosis. Nowadays, the management of atherothrombotic syndromes has gained much attention to defeat atherosclerosis. However, the cost and potential side effects of the existing thrombolytic drugs limiting their use. This study aims to develop a new protocol to screen and say possible action mechanism of a thrombolytic agent trialed and evaluated an anti-atherothrombosis effect of the methanol extract of Dysophyllla auricularia (MDA). For this, hydrogen peroxide (H 2 O 2 )-induced hemolysis (HL) test was conducted to check its antioxidant capacity, while clotlysis (CL) for anti-atherothrombosis in human blood. In the latter case clot was treated with an oxidizer (H 2 O 2 ) and/or antioxidant (ascorbic acid) and/or fibrinolytic (SK: streptokinase) and/or MDA to understand the mode of action of MDA. In HL test, MDA concentration-dependently inhibited HL, where highest inhibition was found 67.87 ± 1.39% at the highest concentration (400 µg/mL) tested. In CL test, MDA was found to antagonize H 2 O 2 , while potentiated SK activity. However, the MDA + SK exerted effect was further reduced by the application of H 2 O 2 . Furthermore, MDA was found to protect the clot by H 2 O 2 -induced oxidizing effect. In conclusion, MDA may protect human erythrocytes from H 2 O 2 -induced damaging effects due to its antioxidative defense capacity, while thrombolytic activity via antioxidant-mediated pro-oxidative effect. The current protocol can be used in non-clinical thrombolytic drug screening.

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“…To look at the protective effect of PHC on the free radical induced oxidative damage on human erythrocytes, in vitro hemolysis assay was performed as adapted by Girish et al (2012) Control was prepared by incubating erythrocyte suspension directly with H 2 O 2 to obtain complete hemolysis and absorbance of supernatant was measured as mentioned. Sample controls were run at every individual concentration of the plant extract by incubating the erythrocyte suspension with plant extract.…”

Section: In Vitro Hemolysis Assaymentioning

confidence: 99%

Evaluation of In Vitro Radical Scavenging and Antihemolytic Efficacy of a Polyherbal Combination Prepared from Traditional Blood Purifiers

Hebbani¹,

Jamadagni²,

Chaitra³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oThe present work is an attempt to look at the possible synergistic role the chosen phytoextracts would contribute in making an effective herbal combination with enhanced anti-oxidant potential. Aqueous extracts of Achyranthes aspera, Amaranthus caudatus, Calotropis procera, Gymnema sylvestre and Taraxacum officinale were examined for their individual phytoconstituent profile and a polyherbal combination was prepared which showed an encouraging result in terms of the radical scavenging and anti-haemolytic activities.

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Protection of DNA and erythrocytes from free radical induced oxidative damage by black gram (Vigna mungo L.) husk extract

Cited by 31 publications

References 31 publications

Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

A Possible Pro-Oxidative Triggered Anti-Atherothrombotic Effect of Dysophylla Auricularia Hydro-Alcoholic Extract

Evaluation of In Vitro Radical Scavenging and Antihemolytic Efficacy of a Polyherbal Combination Prepared from Traditional Blood Purifiers

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