Protection of tilapia (Oreochromis mosambicus) from edwardsiellosis by vaccination with Edwardsiella tarda ghosts

Comparison of the immunogenicity between bacterial ghost and formalin-killed bacteria for Vibrio vulnificus

“…Similar results were reported in tilapia and olive flounder immunized with E. tarda ghost (Kwon et al, 2006(Kwon et al, & 2007,suggesting that VVG has more optimal antigenicity for inducing protective immune responses than FKC.…”

“…Recently, Jung et al (2005) (Kwon et al, 2006(Kwon et al, & 2007Lee et al, 2008;Kwon et al, 2009;Tu et al, 2009).…”

Analysis of hematologic alterations, immune responses and metallothionein gene expression in Nile tilapia (Oreochromis niloticus) exposed to silver nanoparticles

“…Thereafter, 50 lL of a solution containing antigen (FASA, at 10 8 CFU/mL) was loaded into each well. After incubation at 37 C for 24 h, the wells were checked for antigenantibody complex mats; a negative result presented as a white button-like spot at the bottom of the well (Kwon et al 2006). The titer was calculated as the inverse of the final dilution to give rise to the mat product in a well.…”

Generation of Vibrio anguillarum Ghost by Coexpression of PhiX 174 Lysis E gene and Staphylococcal Nuclease A Gene

“…Bacterial ghosts are produced by the controlled expression of bacteriophage PhiX174 lysis gene E, and the E protein leads to the formation of small transmembrane pores through which cytoplasmic contents are expelled [10][11][12][13][14][15]. The potential usefulness of bacterial ghosts as vaccine candidates has recently been reported in a wide variety of mammalian pathogenic Gram-negative bacteria [16][17][18][19] and in a fish pathogenic bacterium, Edwardsiella tarda [20][21][22].…”