Protection of transgenic tobacco plants expressing bovine pancreatic ribonuclease against tobacco mosaic virus

Abstract

Transgenic tobacco plants (Nicotiana tabacum cv. SR1) expressing extracellular pancreatic ribonuclease from Bos taurus and characterized by an increased level of ribonuclease activity in leaf extracts were challenged with tobacco mosaic virus. The transgenic plants exhibited a significantly higher level of protection against the virus infection than the control non-transformed plants. The protection was evidenced by the absence (or significant delay) of the appearance of typical mosaic symptoms and the retarded accumulation of infectious virus and viral antigen. These results demonstrate that modulation of extracellular nuclease expression can be efficiently used in promoting protection against viral diseases.

Dark and light green tissues of tobacco leaves systemically infected with tobacco mosaic virus

“…The accumulation of barley stripe mosaic virus was significantly reduced in transgenic plants expressing a bacterial RNAse (Zhang et al 2001). Transgenic tobacco plants expressing the extracellular pancreatic ribonuclease exhibited a significantly higher level of protection against the virus infection (Trifonova et al 2007). Currently, antiviral mechanisms of RNAses remain to be established.…”

Induced expression of Serratia marcescens ribonuclease III gene in transgenic Nicotiana tabacum L. cv. SR1 tobacco plants

“…In turn, the transgenic expression of the genes of heterologous endoribonucleases of the RNase III family in plants can generate resistance to a number of infections of viral and viroid etiology [3][4][5][6][7][8]. In this Ribonuclease activity, manifestation of symptoms, and level of CP TMV accumulation in leaves of control N. tabacum L. work, the S. lycopersicum anionic peroxidase gene promoter (new for plant genetic engineering) was for the first time used for induced expression of the Serratia marcescens target RNase III gene.…”

“…It was demonstrated in a number of works that the expression in transgenic plants of heterologous RNase genes capable of hydrolysis of similar replicative dsRNA intermediates is a promising strategy for the creation of transgenic plants having a resistance to phytopathogenic viruses and viroids [3][4][5].…”

The Dead Can Nurture: Novel Insights into the Function of Dead Organs Enclosing Embryos

“…michiganensis , a Solanaceae species-pathogenic actinomycete that contains two plasmids, which are important for pathogenesis [35]. In addition, transgenic tobacco plants expressing a bovine pancreatic RNase, an extracellular ribonuclease, showed an increase resistance to plant RNA viruses, namely, Cucumber mosaic virus and Tobacco mosaic virus [36,37]. Notably, treatment of leaves with S-like RNase NE injected into the extracellular space suppresses growth of Phytophthora parasitica [38,39], an oomycete soil borne pathogen with a wide range of host plants.…”