Protective effect of a hydroethanolic extract from Bowdichia virgilioides on muscular damage and oxidative stress caused by strenuous resistance training in rats

Abstract

BackgroundNatural antioxidants can reduce oxidative damage caused by high-intensity resistance training (RT). We investigated the in vitro antioxidant potential of hydroethanolic extract (HEE) from Bowdichia virgilioides on muscular damage and oxidative stress in rats subjected to high-intensity RT.MethodsThirty-two male Wistar rats were divided into four experimental groups: 1) control group (CG), oral administration (P.O.) of vehicle; 2) trained group (TG), vehicle-treated with RT; 3) B. virgilioides untrained group (BVG), treated with B. virgilioides HEE (200 mg/kg P.O.); and 4) trained B. virgilioides group (TBVG), treated with B. virgiliodes HEE (200 mg/kg P.O.). All animals were habituated to the training apparatus for 1 week. CT and TBVG animals were subjected to the training protocol, which consisted of three sets of 10 repetitions with 75% of the load established using the one-repetition maximum, for four weeks. CG and BVG animals were manipulated and fixed to the apparatus three times a week with no load. Treatment with B. virgilioides HEE or vehicle treatment was initiated after 25 days of RT (5 days; one dose per day). At the end of the experiments, plasmatic and gastrocnemius samples from all groups were obtained for the assessment of lipid peroxidation and creatine kinase activity.ResultsCompared to TG rats, TBVG rats showed decreases in plasma and gastrocnemius tissue lipid peroxidation by 55.68% (p <0.0001) and 66.61% (p <0.0012), respectively. Further, compared to TG rats TBVG rats showed decreases in plasma and gastrocnemius tissue oxidative stress by 62.83% (p <0.0005) and 54.97% (p <0.0197), respectively.ConclusionsB. virgilioides HEE treatment reduced markers of oxidative stress caused by high-intensity RT. Further, HEE treatment during training significantly reduced the markers of tissue damage.

Aqueous extract of Bowdichia virgilioides stem bark inhibition of allergic inflammation in mice

“…Interestingly, terpenoids and alkaloids were not observed. Consistent with our findings, previous chemical investigation of B. virgilioides has revealed the presence of phenolic compounds (Arriaga et al, 2000;Dos Santos et al, 2014). Nowadays, phenolic compounds are well known because of their anti-inflammatory properties (Chan et al, 2013;Lu et al, 2014).…”

“…In Brazil, the bark is used in folk medicine to treat different symptoms of inflammatory diseases (Brandao et al, 1992). Previous studies have reported that the extracts from B. virgilioides possess anti-inflammatory (Thomazzi et al, 2010), antinociceptive , and antioxidant (Dos Santos et al, 2014). However, the anti-allergic inflammatory effect of B. virgilioides has not yet been studied.…”

l-glutamine and l-alanine supplementation increase glutamine-glutathione axis and muscle HSP-27 in rats trained using a progressive high-intensity resistance exercise

“…In RE training, ROS/RNS are produced through diverse mechanisms, such as the xanthine and NADP(H) oxidase system, ischemia-reperfusion, prostanoid metabolism and also phagocytic respiratory bursts (Bloomer et al 2005;Goldfarb et al 2005). Although ROS/RNS stimulate important exercise-induced adaptions of the antioxidant and repair systems (Merry et al 2015), data suggests that an accumulation of intense or exhaustive periods of RE (most common in athletes of weightlifting and body builders) can promote an overproduction of ROS/RNS and provoke an increase in oxidative stress (Tanskanen, et al 2010;Kreher and Schwartz 2012;Dos Santos et al 2014). In humans, RE with submaximal intensity caused an excess of physical and oxidative stress, as well as post-exercise immunosuppression (Cakir-Atabek et al 2015).…”

The Acute Effect of Rast Test on Oxidative Stress and Muscle Damage Markers in Young Athletes

“…A Tabela 2 mostra os valores absolutos, potência pico, potência media, potência mínima, índice de fadiga 1 e índice de fadiga 2 dos corredores, obtidos pelo teste anaeróbio máximo. Por fim, no intuito de verificar associações entre as variáveis estudadas, realizou-se uma matriz correlacional, apresentada na Tabela A aptidão anaeróbia é um fator determinante de performance em provas desportivas em que é requerida a manutenção prolongada de grande potência e capacidade anaeróbia para o fornecimento de energia 2 , sendo um componente essencial para o bom desempenho em algumas modalidades esportivas, pois em determinados momentos, há a exigência de um esforço anaeróbio máximo [21][22][23] .…”