Protective Effect of a Mixture of Aloe vera and Silybum marianum Against Carbon Tetrachloride–Induced Acute Hepatotoxicity and Liver Fibrosis

Kim, Sung Hwa; Cheon, Ho Jun; Yun, Nari; Oh, Sun-Tack; Shin, Eunju; Shim, Kyu Suk; Lee, Sun-Mee

doi:10.1254/jphs.08189fp

Cited by 51 publications

(23 citation statements)

References 38 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study Silybum marianum was used for treatment of lipid accumulation in fatty liver induction by tamoxifen. Because SME have a strong protection effect against multiple drugs (10,22), applying SME in TMX-treated rats decreased liver enzymes activities close to normal levels. In addition, the rise of the serum total protein indicated that liver function was improved in the presence of SME.…”

Section: Discussionmentioning

confidence: 97%

Therapeutic Effect of Silybum marianum Plant Extract on Tamoxifen-Induced Fatty Liver in Rats

Behrouj¹,

Ziamajidi²,

Abbasalipourkabir³

et al. 2015

Avicenna J Med Biochem

View full text Add to dashboard Cite

Background: Tamoxifen (TMX) is a synthetic drug that is widely used for treatment of breast cancer. Despite the beneficial effects of TMX, the use of this drug has several side effects such as the development of Non-Alcoholic Fatty Liver Disease (NAFLD) in the patients. Silybum marianum is the most researched plant in treatment of liver disease. Objectives: In this study the effect of Silybum marianum Extract (SME) on histological and biochemical parameters in TMX-treated rats was investigated. Materials and Methods: Adult female Wistar rats were divided to four groups; 1) normal control untreated rats, 2) SME-treated rats, that received only SME in a dose of 1.6 g/kg body weight/day, oral gavage for 14 days, 3) TMX-treated rats, that received TMX in a dose of 1 mg/ kg body weight/day, subcutaneously, for seven days, 4) TMX-SME-treated rats, that received TMX in a dose of 1 mg/kg body weight/day, subcutaneously, for seven days and then SME by oral gavage for 14 days. The groups were compared in terms of serum glucose, triglyceride, cholesterol, Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP), High-Density LipoproteinCholesterol (HDL-C), Low-Density Lipoprotein-Cholesterol (LDL-C), total protein and liver triglyceride amount, and histological findings. Results: Concentrations of HDL-C and protein decreased and activity of ALT increased in TMX group compared with the control group. The SME increased protein concentration and decreased ALT activity in TMX-SME group compared with the TMX group. Histopathological examination and triglyceride assay in liver tissues showed lipid accumulation in the TMX-treated rats and amelioration of lipid accumulation in the liver of TMX-SME-treated rats. Conclusions: Intake of water extract of Silybum marianum is a useful treatment for liver function in drug-induced fatty liver.

show abstract

Section: Discussionmentioning

confidence: 97%

Therapeutic Effect of Silybum marianum Plant Extract on Tamoxifen-Induced Fatty Liver in Rats

Behrouj¹,

Ziamajidi²,

Abbasalipourkabir³

et al. 2015

Avicenna J Med Biochem

View full text Add to dashboard Cite

show abstract

“…Dose of CCl 4 was selected on the basis of a 0.5 ml CCl 4 /kg rat weight diluted in olive oil (1:1 dilution) intraperitonally injection twice per week concentration at which caused oxidative stress and hepatotoxicity administered. 13 Group III (10% CP): the rats received tab water and fed with 10% CP containing diet supplementation. Group IV (0.5 ml CCl4/kg BW + 10% CP): the rats received 0.5 ml CCl 4 /kg rat weight intraperitonally injection twice per week and fed with 10% CP containing diet supplementation.…”

Section: Experimental Designmentioning

confidence: 99%

Nephroprotective Hepatoprotective Potential and Antioxidant Role of Carob Pods (Cerotonia siliqua L.) against Carbon Tetrachloride-induced Toxicity in Rats

Suzek¹,

Çelik²,

Doğan³

2017

IJPER

View full text Add to dashboard Cite

Objective: The aims of current study are the evaluation of the protective effect and antioxidant role of carob pods against carbon tetrachloride (CCl 4 )-induced oxidative stress, hepatotoxicity and nephropathy. Material and Methods: The present experiment was designed as I (control), II (0.5 ml/kg CCl 4 ), III (%10 CP), IV (CCl 4 0.5 ml/kg+%10 CP) groups. While rats in group I and III were fed with a diet without CCl 4 , II, and IV groups received twice 0.5 ml/kg/week, where IV group additionally received %10 CP supplementation for 50 days. The protective roles and antioxidant activity of the CP supplementation feed against CCl 4 -induced oxidative stress and toxicity were evaluated by histopathological changes, measuring hepatic and renal damage biomarkers (HRDB), antioxidant defence system constituents (ADSC) and malondialdehyde (MDA) parameters in the erythrocyte, liver, brain, kidney and spleen tissues of rats. Results: According to the results, the biochemical analysis showed a considerable increase in the serum AST (aspartate aminotransferase), ALT (alanin aminotransferase), GGT (gamma glutamyl transpeptidase) and LDH (lactate dehydrogenase) enzymes, creatinine and urea, and decrease in the group II as compared to that of I group. On contrary, such parameters were decreased in IV group as compared to that of group II. In addition, the results showed that CP supplementation diet restored the CCl 4 induced MDA (malondialdehyde) and ADSC towards to control. The hepatoprotection of CP is further substantiated by the almost normal histological findings in IV group against degenerative changes in II group. Protective effects by CP are further substantiated by the almost normal HRDB for kidney and liver in IV treated group as against degenerative changes in the II treated rats. Conclusion: The results indicated that CP could be as an important as diet-derived antioxidants in preventing oxidative damage in the tissues by reducing the MDA or inhibiting the production of CCl 4 -induced free radicals and liver and kidney destruction.

show abstract

“…In addition, single i.p. administration of CCl 4 induces histological changes including inflammatory cell infiltration in the mouse liver (Kim et al, 2009). Kupffer cell activation is a crucial event in hepatocyte injury induced by CCl 4 and activated Kupffer cells release cytokines such as TNF-α and IL-6, which are important factors for hepatocyte regeneration (Taub et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…In vivo mutagenicity of MeIQx in transgenic mice has also been reported in the liver (Itoh et al, 2000;. Carbon tetrachloride (CCl 4 ) is a potent hepatotoxicant in rodents, and single administration at higher doses causes extensive hepatocellular necrosis with inflammatory cell infiltration (Kim et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Enhancing effects of carbon tetrachloride on in vivo mutagenicity in the liver of mice fed 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)

et al. 2010

View full text Add to dashboard Cite

-Chronic stimulus subsequent to cell injury plays an important role in cancer development, but the precise mechanisms remain unknown partly because appropriate animal models are lacking. In the present study, the effects of hepatotoxicant carbon tetrachloride (CCl 4 ) on in vivo mutagenicity were investigated using gpt delta mice with or without p53. Female B6C3F 1 p53-proficient or -deficient gpt delta mice were given a diet containing 300 ppm of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) for 13 weeks, concurrently with intraperitoneal injection of 1 ml/kg CCl 4 solution once a week. Mutant frequencies of gpt and red/gam in p53-proficient mice fed MeIQx were both significantly elevated by CCl 4 co-treatment. Enhancing effects of CCl 4 treatment were also noted in p53-deficient mice. In the mutation spectra analysis of gpt mutant colonies, G:C to T:A transversions were predominantly observed regardless of CCl 4 injection, and clonal expansion of gpt colonies were increased in the co-treated group as compared with MeIQx alone group. The present data showing no significant changes in mRNA expression levels of CYP1A2 and GSTa4 between MeIQx-treated groups with and without CCl 4 . In the Western blotting analysis, CYP1A2 protein levels were significantly decreased in the co-treated group as compared to MeIQx alone group, and GSTα protein levels were not changed among any groups. It is suggested that the mutant frequency by co-treatment with CCl 4 might result from some factors other than p53 or MeIQx metabolism/excretion. Thus, our data clearly demonstrate that this model could be a powerful tool for identifying the mechanisms underlying combinatorial effects on carcinogenesis.

show abstract

Protective Effect of a Mixture of Aloe vera and Silybum marianum Against Carbon Tetrachloride–Induced Acute Hepatotoxicity and Liver Fibrosis

Cited by 51 publications

References 38 publications

Therapeutic Effect of Silybum marianum Plant Extract on Tamoxifen-Induced Fatty Liver in Rats

Therapeutic Effect of Silybum marianum Plant Extract on Tamoxifen-Induced Fatty Liver in Rats

Nephroprotective Hepatoprotective Potential and Antioxidant Role of Carob Pods (Cerotonia siliqua L.) against Carbon Tetrachloride-induced Toxicity in Rats

Enhancing effects of carbon tetrachloride on in vivo mutagenicity in the liver of mice fed 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)

Contact Info

Product

Resources

About