Protective Effect of a (Poly)phenol-Rich Extract Derived from Sweet Cherries Culls against Oxidative Cell Damage

Abstract

Abstract:Oxidative stress is one of the key phenomena behind the most common types of chronic diseases. Therefore, the modulation of oxidative stress is an interesting target for acting either through prevention or as a therapeutic approach. In this work, a Portuguese variety of cherry (Saco Cherry) was processed in order to obtain a potent in vitro antioxidant phenolic-rich extract (Ch-PRE), which was further explored to evaluate its potential application as nutraceutical agent against cellular oxidative stress damage. Ch-PRE was mainly composed of anthocyanins, particularly cyanidin-3-rutinoside, cyanidin-3-glucoside, peonidin-3-glucoside and neochlorogenic acid, and exhibited a potent chemical antioxidant activity expressed by its oxygen radical absorbance capacity (ORAC) and hydroxyl radical averting capacity (HORAC) values. Ch-PRE also displayed effective intracellular radical scavenging properties in intestinal epithelial and neuronal cells challenged with oxidative stress but showed a different order of effectiveness regarding the modulation of endogenous antioxidant system. Ch-PRE could be an attractive candidate to formulate an agent for the prevention of oxidative stress-induced disorders such as intestinal inflammation disorders or with an appropriated delivery system for neurodegenerative diseases.

Natural polyphenols prevent indomethacin-induced and diclofenac-induced Caco-2 cell death by reducing endoplasmic reticulum stress regardless of their direct reactive oxygen species scavenging capacity

“…Intracellular ROS including superoxide anion, hydrogen peroxide and hydroxyl radical were measured by DHE, DCFH‐DA and HPF assay, respectively . In brief, Caco‐2 cells were treated with specific fluorescent probes (DHE; 10 µ m , DCFH‐DA; 100 µ m or HPF; 10 µ m ) for 30 min, followed by addition of NSAIDs (either INDO or DIC) (1000 µ m ) for 2 h. At the end of treatment period, the cells were lysed with 1% Triton X‐100.…”

“…Intracellular ROS including superoxide anion, hydrogen peroxide and hydroxyl radical were measured by DHE, DCFH-DA and HPF assay, respectively. [10,28] In brief, Caco-2 cells were treated with specific fluorescent probes (DHE; 10 µM, DCFH-DA; 100 µM or HPF; 10 µM) for 30 min, followed by addition of NSAIDs (either INDO or DIC) (1000 µM) for 2 h. At the end of treatment period, the cells were lysed with 1% Triton X-100. The fluorescent intensity was measured by a microplate reader (Wallac 1420 VICTOR 3; PerkinElmer Inc.) at the excitation/emission wavelengths of 470/590 nm (for DHE), 485/535 nm (for DCF) and 510/595 nm (for HPF).…”

Antioxidant Effect of Cocoa By-Product and Cherry Polyphenol Extracts: A Comparative Study

“…Vascular oxidative stress contributes to mechanisms of vascular dysfunction and has been implicated in playing an important role in a number of cardiovascular pathologies [42]. There are also many in vitro studies about the antioxidant properties of Prunus avium L. and cocoa by-products for the prevention of chronic diseases [12,16,43,44]. The presence of hydroxycinnamic acids, flavonoids and anthocyanins made CE interesting for in vitro experiments related to oxidative stress.…”

“…Along with cocoa, cherry fruits have valuable nutritional properties, and their beneficial effects have been demonstrated against oxidative stress damage on both neuronal and intestinal epithelial cells. Similarly to cocoa, the most representative nutraceutical actives in cherries are polyphenols, including flavonoids and anthocyanins [16]. Many studies have used natural extracts on endothelial progenitor cells [17,18] or Human Umbilical Vein Endothelial Cells (HUVECs) [19][20][21] in in vitro experiments related to vascular dysfunction.…”

Genotype, Environment and Management Practices on Red/ Dark-Colored Fruits Phenolic Composition and Its Impact on Sensory Attributes and Potential Health Benefits

“…According to Matias et al [44], a phenolic-rich extract derived from sweet cherries could be an atractive candidate to formulate an agent for the prevention of oxidative stress-induced disorders such as intestinal inlammation disorders. In spite of the large variations in the phenolic compounds content observed among several cherry cultivars, the levels of health-promoting compounds are relevant to human health.…”