Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats

Xue, Meilan; Liu, Ying; Lyu, Rui; Ge, Na; Liu, Man; Ma, Yan; Liang, Hui

doi:10.1371/journal.pone.0178684

Cited by 19 publications

(16 citation statements)

References 42 publications

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“…Ge et al also reported that aplysin had the hepatoprotective effect on oxidative damage caused by ethanol, which could prevent alcoholic liver injury [ 13 ]. Xue et al showed that aplysin could protect ethanol-induced hepatic injury, repair barrier function of the intestine and provide a low inflammatory response and a low level of plasma endotoxin [ 15 ]. Another study from a Chinese journal showed that the intestinal mucosal damage and lipid metabolism disorder caused by excessive iron exposure in rats were protected by aplysin [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Intestinal health was investigated and discussed by measuring the barrier function of intestinal mucosa and the level of plasma endotoxin. Alcohol induced the damage to the barrier function of intestinal mucosa, increased intestinal permeability resulted from endotoxin and other adverse factors, and contributed to the endotoxemia in alcoholic liver damage [ 15 , 18 ]. In this study, the plasma endotoxin level was significantly higher in the co-treatment of ethanol plus iron group.…”

Section: Discussionmentioning

confidence: 99%

“…DAO and D-LA are prevented from infiltratrating the portal blood, providing a barrier function by intact intestinal mucosa, so they are indices of high intestinal permeability [ 19 ]. Some studies documented that DAO activity and the D-LA level in the chronic ethanol intake were significantly increased [ 15 , 20 ]. Furthermore, decreasing the DAO activities and D-LA and FABP2 levels in plasma were observed after aplysin administration in the study.…”

Section: Discussionmentioning

confidence: 99%

“…Aplysin potent biological activities include antitumor, anti-inflammatory, immunostimulation and antioxidant activities [ 14 ]. Previous study in our lab has demonstrated that a high dose aplysin treatment (150 mg kg −1 day −1 ) can significantly protect alcoholic liver injury [ 13 ] by repairing intestinal barrier function, normalizing fecal microbiota, and reducing inflammatory response and plasma endotoxin level in rats [ 15 ]. However, the potential effect of aplysin on the intestinal microflora and barrier function in rats exposed to iron and ethanol has not been explored.…”

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron

Liu

et al. 2018

Nutrients

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Aplysin, a kind of phytochemicals or phytonutrients, is purified from red alga Laurencia tristicha. The present study aims to investigate the influence of aplysin on changes of intestinal permeability and microbiota induced by excessive ethanol and iron. Thirty male rats were randomly divided into three groups (10/group): control group (normal saline); ethanol + iron group as EI treated with ethanol (8–12 mL/kg/day) and iron (1000 mg/kg) in diet; EI supplemented with aplysin (150 mg/kg/day) group as AEI; the trial lasts for 12 weeks. The result showed that levels of plasma endotoxin, fatty acid-binding protein 2, D-lactic acid, diamine oxidase were increased in rats in the EI group; and significantly decreased by 14%, 17%, 26%, 16%, respectively (p < 0.05) in the AEI group after the 12-week aplysin treatment. Moreover, in the AEI group the amount of Escherichia coli and Bacteroides fragilis were higher, while the amount of Lactobacillus, Bifidobacterium and Clostridium were lower than those in the EI group. The expressions of iron transporters divalent-metal transporter 1(DMT1) and ferroportin 1(FPN1) were significantly upregulated in the EI group compared to those in the control group. In conclusion, aplysin could effectively improve intestinal permeability and intestinal flora disorder induced with excessive ethanol and iron.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron

Liu

et al. 2018

Nutrients

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“…Additionally, it inhibited apoptosis of enterocytes and modulated tight junction protein claudin-1, leading to improved gut integrity and endotoxemia; it also restored the antioxidant capacity of the liver and blocked the release of free fatty acids. Furthermore, aplysin from the red alga Laurencia tristicha was reported to revert the increased plasma endotoxin diamine oxidase (DAO) and fatty acid-binding protein 2 (FABP2) as well as the altered gut microbial composition in rats with ALD [ 85 ]. In another study, when administrated in a double layered microencapsulation, L. plantarum was shown to attenuate endotoxemia, normalize transaminases, reduce NF-κB and other cytokines, and restore the morphology and function of the gut and liver [ 27 ].…”

Section: Alcoholic Liver Disease (Ald)mentioning

confidence: 99%

Gut Microbiota’s Relationship with Liver Disease and Role in Hepatoprotection by Dietary Natural Products and Probiotics

Meng

et al. 2018

Nutrients

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A variety of dietary natural products have shown hepatoprotective effects. Increasing evidence has also demonstrated that gut microorganisms play an important role in the hepatoprotection contributed by natural products. Gut dysbiosis could increase permeability of the gut barrier, resulting in translocated bacteria and leaked gut-derived products, which can reach the liver through the portal vein and might lead to increased oxidative stress and inflammation, thereby threatening liver health. Targeting gut microbiota modulation represents a promising strategy for hepatoprotection. Many natural products could protect the liver from various injuries or mitigate hepatic disorders by reverting gut dysbiosis, improving intestinal permeability, altering the primary bile acid, and inhibiting hepatic fatty acid accumulation. The mechanisms underlying their beneficial effects also include reducing oxidative stress, suppressing inflammation, attenuating fibrosis, and decreasing apoptosis. This review discusses the hepatoprotective effects of dietary natural products via modulating the gut microbiota, mainly focusing on the mechanisms of action.

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Effect of nicotinamide riboside on lipid metabolism and gut microflora‐bile acid axis in alcohol‐exposed mice

Xue

Liu

et al. 2020

Food Science & Nutrition

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Alcoholic liver disease (ALD) is the most common complication of alcohol abuse, while we lack safe and effective treatment for ALD. This study aimed to explore the effects of nicotinamide riboside (NR) on lipid metabolism and gut microflora‐bile acid axis in alcohol‐exposed mice. NR significantly improved liver histopathological damage and abnormal liver function. NR as a provider of nicotinamide adenine dinucleotide (NAD+) increased the NAD+/NADH ratio. Meanwhile, NR inhibited the activation of the protein phosphatase 1 signaling pathway, decreased the liver triglyceride and total bile acid levels, and reduced lipid accumulation. According to the results of gut microflora species analysis, NR intervention changed the microbial community structure at the phylum, family and genus levels, and the species abundances returned to a level similar to these of the normal control group. Besides, the results of high‐performance liquid chromatograph‐mass spectrometry showed that NR intervention resulted in fecal bile acid levels tending to be normal with decreased chenodeoxycholic acid level and increased deoxycholic acid and hyocholic acid levels. Spearman's correlation analysis showed a correlation between gut microflora and bile acids. Therefore, NR supplementation has the potential to prevent ALD, and its mechanism may be related to regulating lipid metabolism disorders and the gut microflora‐bile acid axis.

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Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats

Cited by 19 publications

References 42 publications

Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron

Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron

Gut Microbiota’s Relationship with Liver Disease and Role in Hepatoprotection by Dietary Natural Products and Probiotics

Effect of nicotinamide riboside on lipid metabolism and gut microflora‐bile acid axis in alcohol‐exposed mice

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