Protective effect of desferrioxamin against TPA caused inflammation in CD-1 mouse skin

Soybir, Gürsel; Koyuncu, Hülya; Köksoy, Ferda Nihat; Yalçın, Orhan; Özşeker, A.; Alatlı, Canan; Topuzlu, Cemalettin

doi:10.1016/s0960-7404(96)80029-6

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…We anticipate that the protective effect of DFO by the above mentioned mechanisms limits tissue injury and the perpetuated recruitment of activated neutrophils characteristic for chronic venous leg ulcers. In fact, in a TPA (12-O-tetradecanoyl-phorbol-13-acetate) induced in¯ammation model, topical application of DFO prior to TPA treatment could almost completely inhibit the neutrophil in¯ux into the interstitial tissue of mouse ears (Soybir et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Selective Pick-Up of Increased Iron by Deferoxamine-Coupled Cellulose Abrogates the Iron-Driven Induction of Matrix-Degrading Metalloproteinase 1 and Lipid Peroxidation in Human Dermal Fibroblasts In Vitro: A New Dressing Concept

Wenk¹,

Foitzik

Achterberg

et al. 2001

Journal of Investigative Dermatology

102

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Using atomic absorption spectrum analysis, we found iron levels in exudates from chronic wounds to be significantly increased (3.71 +/- 1.56 micromol per g protein) compared to wound fluids from acute wounds derived from blister fluids (1.15 +/- 0.62 micromol per g protein, p < 0.02), drainage fluids of acute wounds (0.87 +/- 0.34 micromol per g protein, p < 0.002), and pooled human plasma of 50 volunteers (0.42 micromol per g protein). Increased free iron and an increase in reactive oxygen species released from neutrophils represent pathogenic key steps that --via the Fenton reaction - are thought to be responsible for the persistent inflammation, increased connective tissue degradation, and lipid peroxidation contributing to the prooxidant hostile microenvironment of chronic venous leg ulcers. We herein designed a selective pick-up dressing for iron ions by covalently binding deferoxamine to cellulose. No leakage occurred following gamma sterilization of the dressing and, more importantly, the deferoxamine-coupled cellulose dressing retained its iron complexing properties sufficient to reduce iron levels found in chronic venous ulcers to levels comparable to those found in acute wounds. In order to study the functionality of the dressing, human dermal fibroblasts were exposed to a Fenton reaction mimicking combination of 220 microM Fe(III) citrate and 1 mM ascorbate resulting in a 4-fold induction of matrix-degrading metalloproteinase 1 as determined by a matrix-degrading metalloproteinase 1 specific enzyme-linked immunosorbent assay. This induction was completely suppressed by dissolved deferoxamine at a concentration of 220 microM or by an equimolar amount of deferoxamine immobilized to cellulose. In addition, the Fe(III) citrate and ascorbate driven Fenton reaction resulted in an 8-fold increase in malondialdehyde, the major product of lipid peroxidation, as determined by high pressure liquid chromatography. This increase in malondialdehyde levels could be significantly reduced in the presence of the selective pick-up dressing coupled with deferoxamine suggesting that the deferoxamine dressing, in fact, prevents the development of a damaging prooxidant microenvironment and also protects from unfavorable consequences like matrix-degrading metalloproteinase 1 and lipid peroxide induction.

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Section: Discussionmentioning

confidence: 99%

Selective Pick-Up of Increased Iron by Deferoxamine-Coupled Cellulose Abrogates the Iron-Driven Induction of Matrix-Degrading Metalloproteinase 1 and Lipid Peroxidation in Human Dermal Fibroblasts In Vitro: A New Dressing Concept

Wenk¹,

Foitzik

Achterberg

et al. 2001

Journal of Investigative Dermatology

102

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“…For this purpose mice were pretreated (24h before acute UVB irradiation) with DFO or DFP (150 nmol/cm 2 of skin; dissolved in physiological saline and 0.5% carbopol) (these doses are high enough to ensure a fully effective removal of mouse skin labile iron (see e.g. [35]). DFO and DFP significantly decreased by 44 + 8 % and 30 + 8 %, respectively, the acute UVB (360 mJ/cm 2 )-induced skin edema (% skin fold as in Fig.…”

Section: Effect Of Stilbenes On Acute Uvb Radiation-induced Skin Damagesmentioning

confidence: 99%

Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis

Sirerol

Feddi

Mena

et al. 2015

Free Radical Biology and Medicine

107

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“…Model justification. Compared to other animal models, CD-1 mice were found to be highly susceptible to induction of cutaneous inflammation following exposure to croton oil (4,10), lipopolysaccharide (11), 12-O-tetradecanoylphorbol-13-acetate (TPA) (12), and petroleum distillates (13). High levels of inflammation were found when compounds were applied topically or administered as dietary component (14)(15)(16)(17).…”

mentioning

confidence: 99%

Antagonism of croton oil inflammation by topical emu oil in CD‐1 mice

Yoganathan

Nicolosi

Wilson

et al. 2003

Lipids

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Emu oil is derived from the emu (Dromaius novaehollandiae), which originated in Australia, and has been reported to have anti-inflammatory properties. Inflammation was induced in anesthetized CD-1 mice by applying 50 microL of 2% croton oil to the inner surface of the left ear. After 2 h, the area was treated with 5 microL of emu, fish, flaxseed, olive, or liquified chicken fat, or left untreated. Animals were euthanized at 6 h postapplication of different oils, and earplugs (EP) and plasma samples were collected. Inflammation was evaluated by change in earlobe thickness, increase in weight of EP tissue (compared to the untreated ear), and induction in cytokines interleukin (IL)-1alpha and tumor necrosis factor-alpha (TNF-alpha) in EP homogenates. Although reductions relative to control (croton oil) were noted for all treatments, auricular thickness and EP weights were significantly reduced (-72 and -71%, respectively) only in the emu oil-treated group. IL-1alpha levels in homogenates of auricular tissue were significantly reduced in the fish oil (-57%) and emu oil (-70%) groups relative to the control group. The cytokine TNF-alpha from auricular homogenates was significantly reduced in the olive oil (-52%) and emu oil (-60%) treatment groups relative to the control group. Plasma cytokine levels were not changed by croton oil treatment. Although auricular thickness and weight were significantly correlated with each other (r = 0.780, P < 0.003), auricular thickness but not weight was significantly correlated with cytokine IL-alpha (r = 0.750, P < 0.006) and TNF-alpha (r = 0.690, P < 0.02). These studies indicate that topical emu oil has anti-inflammatory properties in the CD-1 mouse that are associated with decreased auricular thickness and weight, and with the cytokines IL-1alpha and TNF-alpha.

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Protective effect of desferrioxamin against TPA caused inflammation in CD-1 mouse skin

Cited by 6 publications

References 16 publications

Selective Pick-Up of Increased Iron by Deferoxamine-Coupled Cellulose Abrogates the Iron-Driven Induction of Matrix-Degrading Metalloproteinase 1 and Lipid Peroxidation in Human Dermal Fibroblasts In Vitro: A New Dressing Concept

Selective Pick-Up of Increased Iron by Deferoxamine-Coupled Cellulose Abrogates the Iron-Driven Induction of Matrix-Degrading Metalloproteinase 1 and Lipid Peroxidation in Human Dermal Fibroblasts In Vitro: A New Dressing Concept

Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis

Antagonism of croton oil inflammation by topical emu oil in CD‐1 mice

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