Protective effect of leptin against ischemia-reperfusion injury in the rat small intestine

Hacioğlu, Alper; Algin, Cem; Paşaoğlu, Özgül; Paşaoğlu, Ercüment; Kanbak, Güngör

doi:10.1186/1471-230x-5-37

Cited by 34 publications

(33 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protective effect of leptin in intestinal IR injury has recently been demonstrated [24,25]. Hacioglu et al performed intestinal IR injury in adult Wistar rats weighting 200-250 g, but we used 140±14.5 g rats, aged 8 weeks in our study [24]. In contrast to our study, they did not report any significant difference in intestinal injury to the ileum between IR + leptin group and IR group.…”

Section: Discussioncontrasting

confidence: 54%

“…Kimura et al have suggested that arterial relaxation by leptin is mediated by NO released from the endothelium [23]. The protective effect of leptin in intestinal IR injury has recently been demonstrated [24,25]. Hacioglu et al performed intestinal IR injury in adult Wistar rats weighting 200-250 g, but we used 140±14.5 g rats, aged 8 weeks in our study [24].…”

Section: Discussionmentioning

confidence: 98%

“…In contrast to our study, they did not report any significant difference in intestinal injury to the ileum between IR + leptin group and IR group. This may be because of the short (2 h) reperfusion they performed [24]. Sukhotnik et al showed the protective effect of leptin in rats that underwent 30-min mesenteric ischemia followed by 24 h of reperfusion, but they administered leptin at a dose of 50 μg/kg once a day for 48 h before and 24 h following IR [25].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Leptin on Intestinal Ischemia–Reperfusion Injury

et al. 2013

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Leptin on Intestinal Ischemia–Reperfusion Injury

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Leptin has been reported to act as a mitogenic agent and stimulates the growth of several obesity-associated advanced human cancers, including breast cancer, 25 colon cancer, 26 endometrial cancer 27 and renal cancer. 28 The use of leptin in these patients to prevent chemotherapy-induced mucositis should be probably avoided.…”

Section: Discussionmentioning

confidence: 99%

Leptin accelerates enterocyte turnover during methotrexate-induced intestinal mucositis in a rat model

Sukhotnik

Shteinberg

Karry

et al. 2009

Cancer Biology & Therapy

View full text Add to dashboard Cite

Gastrointestinal mucositis occurs as a consequence of cytotoxic treatment. In the present study, we tested whether leptin can protect gut epithelial cells from methotrexate (MTX)-induced intestinal damage. Non-pretreated and pretreated with MTX Caco-2 cells were incubated with increasing concentrations of leptin for 24 h. Cell proliferation and apoptosis were assessed using FACS analysis. Adult rats were divided into three experimental groups: Control rats; MTX-rats were treated with a single dose of MTX, and MTX-LEP rats were also treated with leptin for 3 d. Intestinal mucosal damage (Park score), mucosal structural changes (bowel and mucosal weight, mucosal DNA and protein content, villus height and crypt depth), enterocyte proliferation, and enterocyte apoptosis were measured at sacrifice. RT-PCR was used to determine the level of bax and bcl-2 mRNA expression. In the vitro experiment, treatment with leptin of Caco-2 cells pre-treated with MTX resulted in a significant stimulation of cell proliferation and inhibition of cell apoptosis in a dose-dependent manner. In the vivo experiment, MTX-LEP rats demonstrated a greater jejunal and ileal bowel and mucosal weight, mucosal DNA and protein, villus height and crypt depth, as well as a greater enterocyte proliferation index compared to MTX-animals. MTX-LEP rats also showed a trend toward an increase in enterocyte apoptosis that was accompanied by an increase in bax mRNA and decrease in bcl-2 mRNA expression. In conclusion, leptin enhances proliferation and decreases apoptosis in Caco-2 cells pretreated with MTX. In a rat model of MTX-induced mucositis, treatment with leptin improves intestinal recovery and enhances enterocyte turnover.

show abstract

“…Furthermore, leptin induces aromatase expression in adipose tissue followed by the elevation of estrogen (30); estrogen and its receptors are involved in learning, so the effect of leptin on memory may in part be due to its effects on estrogen (31) through the increased amounts of nitric oxide and acetylcholine in the brain (32). Leptin also triggers the release of NO in some tissues including the hypothalamus, anterior pituitary gland (33) and mesenteric vasculature (34). Since leptin receptors are also present in the hippocampus, it is possible that leptin, through NO, plays a helpful role in memory in the hippocampus.…”

Section: Figmentioning

confidence: 99%

Beneficial Effect of Leptin on Spatial Learning and Memory in Streptozotocin-Induced Diabetic Rats

Ghasemi¹,

Zendehbad²,

Zabihi³

et al. 2016

Balkan Med J

View full text Add to dashboard Cite

Background: Diabetes mellitus is a chronic disease which may be accompanied by cognitive impairments. The expression of the obesity gene (ob) is decreased in insulin-deficient diabetic animals and increased after the administration of insulin or leptin. Plasma leptin levels are reduced in the streptozotocin (STZ)-induced diabetic rats. Therefore, the deleterious effects of diabetes on memory may be due to the reduction of leptin. Aims: Investigate the effect of subcutaneous injection of leptin on spatial learning and memory in STZ-induced diabetic rats. Study Design: Animal experimentation. Methods: The rats were divided into three groups: 1-control, 2-diabetic, and 3-diabetic-leptin. Diabetes was induced in groups 2 and 3 by STZ injection (55 mg/kg) intraperitoneally (i.p). The animals received leptin (0.1 mg/kg) or saline subcutaneously (s.c) for 10 days before behavioral studies. Then, they were examined in the Morris water maze over 3 blocks after 3 days of the last injection of leptin. Results: The travelled path length and time spent to reach the platform significantly increased in the diabetic group (p<0.001) and decreased with leptin treatment (p<0.01 & p<0.001 respectively); also, a significant increase in path length and time was observed between the diabetic-leptin group and the diabetic group (p<0.01, p<0.001, respectively) in the probe test. Conclusion: Leptin can exert positive effects on memory impairments in diabetic rats. Keywords: Leptin, spatial memory, streptozotocin

show abstract

Protective effect of leptin against ischemia-reperfusion injury in the rat small intestine

Cited by 34 publications

References 36 publications

Effects of Leptin on Intestinal Ischemia–Reperfusion Injury

Effects of Leptin on Intestinal Ischemia–Reperfusion Injury

Leptin accelerates enterocyte turnover during methotrexate-induced intestinal mucositis in a rat model

Beneficial Effect of Leptin on Spatial Learning and Memory in Streptozotocin-Induced Diabetic Rats

Contact Info

Product

Resources

About