Protective effect of Lycium Barbarum polysaccharides on dextromethorphan-induced mood impairment and neurogenesis suppression

Po, Kevin Kai-ting; Leung, Joseph Wai-Hin; Chan, Jim Yee Him; Fung, Timothy Kai-Hang; Sánchez-Vidaña, Dalinda Isabel; Sin, Emily L.L.; So, Kwok‐Fai; Lau, Benson Wui-Man; Siu, Andrew M. H.

doi:10.1016/j.brainresbull.2017.06.014

Cited by 31 publications

(13 citation statements)

References 33 publications

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“…Lycium barbarum polysaccharide (LBP) is a macromolecular substance with a variety of biological activities extracted from the traditional Chinese herb Lycium barbarum . LBP has functions of antitumor, antioxidation, anti-free radicals, sugar lipid-lowering and other pharmacological activities [9,10]. Previous study showed that LBP could bind to the polysaccharide receptors, which was present on the surface of dendritic cells (DCs) [11], and that activated DCs and their released active factors indirectly affect T cells to improve their activities, thus, T cells are transformed into killer T cells to increase the killing ability of T cells [12].…”

Section: Introductionmentioning

confidence: 99%

Lycium barbarum polysaccharide induced apoptosis and inhibited proliferation in infantile hemangioma endothelial cells via down-regulation of PI3K/AKT signaling pathway

Lou

Chen²,

Zhong

et al. 2019

Bioscience Reports

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Lycium barbarum polysaccharide (LBP) has a variety of pharmacological and biological activities such as anti-inflammatory, antioxidation, anti-apoptosis, immune regulation and other pharmacological effects; however, the effect of LBP on infantile hemangioma (IH) was less reported. Primary human hemangioma endothelial cells (HemECs) were isolated from fresh surgical specimens of patients. HemECs was treated with LBP and the changes in proliferative and apoptotic signaling pathways were investigated by performing cell counting kit-8, cloning formation experiment, in vitro angiogenesis experiment, flow cytometry, Western blot, immunofluorescence, HE stain and real-time quantitative polymerase chain reaction. We found that LBP potently inhibited the proliferation of HemECs and achieved a low-micromolar IC50 (45 and 40 μg/ml, the half maximal inhibitory concentration) value and less angiogenesis, however, the IC50 had no effect on human umbilical vein endothelial cells (HUVECs) viability. LBP treatment induced apoptosis in HemECs, which was supported by positive Annexin-V-FITC staining, the activation of cleaved caspase-3 and Bcl-2-associated X protein (Bax) and the inhibition of B-cell lymphoma/leukemia-2 (Bcl-2). Moreover, the result demonstrated that LBP suppressed the expressions of proliferating cell nuclear antigen (PCNA), Ki67, vascular endothelial growth factor (VEGF), VEGFR2 and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signal pathway. PI3K-specific agonist (IGF-1) had promotive effects on HemECs proliferation, which was reversed by LBP. Our study suggests that the effectiveness of LBP in IHs may be associated with its potent anti-proliferative and apoptotic activities in HemECs. Thus, our findings may provide an effective medicine for IHs treatment.

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Section: Introductionmentioning

confidence: 99%

Lycium barbarum polysaccharide induced apoptosis and inhibited proliferation in infantile hemangioma endothelial cells via down-regulation of PI3K/AKT signaling pathway

Lou

Chen²,

Zhong

et al. 2019

Bioscience Reports

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“…Lycium barbarum polysaccharides (LBPs) are the main effective ingredient of Lycium barbarum, comprised of glucose, arabinose, galactose, mannose, xylose, rhamnose and fucose (Zeng et al 2019;Liu et al 2020). Studies have demonstrated that LBPs have multiple pharmacological functions, including antioxidant, anti-inflammatory, antitumor, immune regulation, neuroprotective, hypoglycaemic and hypolipidemic effects (Li XM et al 2007;Miao et al 2010;Cheng et al 2015;Xing et al 2016;Po et al 2017;Tang ZY et al 2017;Pop et al 2020). Moreover, Li J et al (2017) demonstrated the protective effects of LBPs in renal damage.…”

Section: Introductionmentioning

confidence: 99%

Lycium barbarum polysaccharides protect mice from hyperuricaemia through promoting kidney excretion of uric acid and inhibiting liver xanthine oxidase

Zhang

Zhang³

et al. 2020

Pharmaceutical Biology

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Context: Lycium barbarum L. (Solanaceae) polysaccharides (LBPs) are important active constituents that have demonstrated kidney protection. Objective: This study investigated the effect of LBPs on hyperuricaemia and explored the underlying mechanism in mice. Materials and methods: Thirty-six C57BL/6 mice were randomly divided into the control group, hyperuricaemia group, allopurinol group (5 mg/kg) and three LBP groups (n ¼ 6). The LBP groups were treated orally with LBPs at 50, 100 and 200 mg/kg body weight for 7 days. We examined the levels of serum uric acid (S UA) and urinary uric acid (U UA), as well as xanthine oxidase (XOD) activities. mRNA and protein were quantified by quantitative real-time PCR and Western blotting, respectively. Results: LBPs treatment (100 and 200 mg/kg) significantly reduced the S UA levels to 4.83 and 4.48 mg/dL, and markedly elevated the U UA levels to 4.68 and 5.18 mg/dL (p < 0.05), respectively, while significantly increased the mRNA and protein expression levels of renal organic anti-transporter 1 (OAT1) and organic anti-transporter 3 (OAT3), and markedly decreased the levels of glucose transporter 9 (GLUT9) (p < 0.05). Additionally, the serum XOD activities were reduced to 31.5 and 31.1 mU/mL, and the liver XOD activities were reduced to 80.6 and 75.6 mU/mL after treatment with 100 and 200 mg/kg LBPs (p < 0.01), respectively. Discussion and conclusions: This study demonstrated the potential role of LBPs in reducing the uric acid level in hyperuricemic mice. A border study population should be evaluated. These results are valuable for the development of new anti-hyperuricaemia agents from LBPs.

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“…Berry polyphenols modulate the GM, induce beneficial short‐chain fatty acid (SCFA) production and prevent inflammatory stress (Wu et al , 2018). Furthermore, as a compound with important biological activities, LB polysaccharide (LBP) has been reported to have potential antioxidant activity and immunomodulatory effects, as well as hypoglycaemic, hypolipidaemic and neuroprotective effects (Yang et al , 2015; Po et al , 2017; Masci et al , 2018; Ding et al , 2019a). Kulczyński & Gramza‐Michałowska (2016) showed that LBP increased the activity of antioxidant enzymes in kidneys, lungs and liver, and scavenged free radicals (superoxide anion and hydroxyl radicals) in vitro .…”

Section: Introductionmentioning

confidence: 99%

Dietary whole Goji berry (Lycium barbarum) intake improves colonic barrier function by altering gut microbiota composition in mice

Tian

Zhang

Zhao

et al. 2020

Int J of Food Sci Tech

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Diet strongly affects human health by modulating gut microbiota (GM) composition. Lycium barbarum (LB), which contains polysaccharides and polyphenols, can alter the GM and intestinal barrier function, thereby reducing and preventing the occurrence of intestinal diseases. To investigate how LB improves colonic barrier function, male C57BL/6J mice were fed diets containing 1.5% or 3% LB for 10 weeks. Results of HiSeq 16S rDNA analysis showed that LB markedly altered microbial profile by supporting the growth of Verrucomicrobia, Bacteroidetes, Bacteroidales_S24-7_group, Anaerotruncus, Coprococcus_1, Ruminococcaceae_UCG-014 and Akkermansia, while suppressing Firmicutes, Helicobacter, Bacteroides and Mucispirillum. LB supported the short-chain fatty acid (SCFA)-producing bacteria, and the SCFA concentrations increased, while the ammonia concentrations and pH values in faeces decreased, thereby increasing the expression of tight junction proteins and mucin, subsequently improving intestinal function and histomorphology. Dietary LB, as a functional dietary component, enhanced colonic barrier function and improved gut health by altering GM composition.

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Protective effect of Lycium Barbarum polysaccharides on dextromethorphan-induced mood impairment and neurogenesis suppression

Cited by 31 publications

References 33 publications

Lycium barbarum polysaccharide induced apoptosis and inhibited proliferation in infantile hemangioma endothelial cells via down-regulation of PI3K/AKT signaling pathway

Lycium barbarum polysaccharide induced apoptosis and inhibited proliferation in infantile hemangioma endothelial cells via down-regulation of PI3K/AKT signaling pathway

Lycium barbarum polysaccharides protect mice from hyperuricaemia through promoting kidney excretion of uric acid and inhibiting liver xanthine oxidase

Dietary whole Goji berry (Lycium barbarum) intake improves colonic barrier function by altering gut microbiota composition in mice

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