Protective effect of Ugni molinae Turcz against oxidative damage of human erythrocytes

Suwalsky, Mario; Orellana, P.; Avello, Marcia; Villena, Fernando Pardo-Manuel de

doi:10.1016/j.fct.2006.08.010

Cited by 67 publications

(50 citation statements)

References 24 publications

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“…The lack of essential changes with respect to the control in this interphase region could mean that the compounds interact with the membrane quite shallowly, and this interaction has an interfacial character. A similar character of the interaction between polyphenolic compounds and membranes was documented in other studies [16,17,21]. This conclusion is confirmed by the fluorimetric studies with the Laurdan and Prodan probes.…”

Section: Discussionsupporting

confidence: 77%

“…The antioxidant activity of some flavonoids, including anthocyanin, is markedly higher than the activity of vitamin E, and its synthetic equivalent Trolox ® . They also effectively inhibit erythrocyte hemolysis induced by free radicals, protecting the erythrocyte membrane against oxidative destruction [5,10,[13][14][15][16][17][18]]. An exposed and very good site of attack by free radicals in an organism is the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of selected anthocyanins with erythrocytes and liposome membranes

Bonarska-Kujawa

Pruchnik

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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Anthocyanins are one of the main flavonoid groups. They are responsible for, e.g., the color of plants and have antioxidant features and a wide spectrum of medical activity. The subject of the study was the following compounds that belong to the anthocyanins and which can be found, e.g., in strawberries and chokeberries: callistephin chloride (pelargonidin-3-O-glucoside chloride) and ideain chloride (cyanidin-3-O-galactoside chloride). The aim of the study was to determine the compounds' antioxidant activity towards the erythrocyte membrane and changes incurred by the tested anthocyanins in the lipid phase of the erythrocyte membrane, in liposomes composed of erythrocyte lipids and in DPPC, DPPC/cholesterol and egg lecithin liposomes. In particular, we studied the effect of the two selected anthocyanins on red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC and DPPC/cholesterol liposomes. Fluorimetry with the Laurdan and Prodan probes indicated increased packing density in the hydrophilic phase of the membrane in the presence of anthocyanins. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The compounds slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The study has shown that both anthocyanins are incorporated into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The investigation proved that the compounds penetrate only the outer part of the external lipid layer of liposomes composed of erythrocyte lipids, DPPC, DPPC/cholesterol and egg lecithin lipids, changing its packing order. Fluorimetry studies with DPH-PA proved that the tested anthocyanins are very effective antioxidants. The antioxidant activity of the compounds was comparable with the activity of Trolox ® .

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Bonarska-Kujawa

Pruchnik

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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“…Earlier studies were focused on determining the main phenolic compounds, antioxidant capacity and mechanisms of action of murtilla leaves at a cellular level (Avello & Pastene, 2005;Rubilar et al, 2006;Suwalsky et al, 2007). Recently Ruiz et al (2010) demonstrated that the content of flavonols and polyphenols in this fruit is approximately 32 ± 4mg of gallic acid/100g of the fruit.…”

Section: Introductionmentioning

confidence: 99%

Phenolic compounds and antioxidant activity of hydroalcoholic extracts of wild and cultivated murtilla (Ugni molinae Turcz.)

Augusto¹,

Scheuermann²,

Alencar³

et al. 2014

Food Sci. Technol (Campinas)

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Over the last decade a considerable increase in the number of studies addressing the use of antioxidants from natural sources has led to the identification and understanding of the potential mechanisms of biologically active components. This results from the fact that they can be used to replace synthetic antioxidants commonly used in food. Murtilla (Ugni molinae Turcz) is a native berry grown in Chile, and in the present study, the phenolic composition and antioxidant activity of its fruits were studied. Hydroalcoholic extracts of dehydrated fruits from two genotypes of murtilla (Ugni molinae Turcz.) were produced. Extracts of wild murtilla and 14-4 genotype fruits had 19.35 and 40.28mg GAE/g for Total Phenolic Compounds, 76.48, and 134.35μmol TEAC/g for DPPH, and 157.04 and 293.99 μmol TEAC/g for ABTS, respectively. Components such as quercetin, epicatechin, and gallic, benzoic and hydrocaffeic acids were identified by CG/MS analysis. All of them showed antioxidant activity. Therefore, it is possible to say that the hydroalcoholic extracts of murtilla have antioxidant potential to be used in lipidic food.

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“…DOI: 10.3109/13880209.2014.1001407 Another important ROS produced by neutrophils is HOCl, and the reaction is catalyzed by MPO at sites of inflammation (Aruoma, 1997). Despite the fact that HOCl is the most known oxidant bactericidal produced by neutrophils (Hampton et al, 1998), this ROS can cause changes in membrane fluidity and membrane morphological transformations and has been reported to play a role in a wide range of human inflammatory diseases, including atherosclerosis, asthma, rheumatoid arthritis, cystic fibrosis, acne, psoriasis, and some cancers (Bickers & Athar, 2006;Davies et al, 2008;Okayama, 2005;Suwalsky et al, 2007). Considering the importance of evaluating the effect of these two most promising V. cauliflora extracts in neutrophils-induced HOCl, and, for this purpose the specific probe for HOCL detection, APF, was used.…”

Section: Resultsmentioning

confidence: 99%

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

Ribeiro

Berto

Ribeiro

et al. 2015

Pharmaceutical Biology

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(2015) Stem bark and flower extracts of Vismiacauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes, Pharmaceutical Biology, 53:11, 1691-1698, DOI: 10.3109/13880209.2014 Previous research on V. cauliflora extracts suggests its potential to neutralize cellular oxidative damages related to the production of reactive oxygen and nitrogen species.Objective: To determine the activity of stem bark and flower extracts of V. cauliflora on the modulation of oxidative burst in human neutrophils, as well as its potential to inhibit oxidative damage in human erythrocytes. Materials and methods:The modulation of neutrophil's oxidative burst by the ethanolic extracts (0.3-1000 mg/mL) was determined by the oxidation of specific probes by reactive species. Additionally, the potential of these extracts to inhibit oxidative damage in human erythrocytes was evaluated by monitoring its biomarkers of oxidative stress. Results: Vismia cauliflora extracts presented remarkable capacity to prevent the oxidative burst in activated human neutrophils (IC 50 515 mg/mL). However, the maximum percentage of inhibition achieved against hydrogen peroxide was 45%. Concerning the oxidative damage in human erythrocytes, the extracts were able to minimize the tert-butyl hydroperoxide-induced hemoglobin oxidation and lipid peroxidation in a very low concentration range (2.7-18 mg/mL). Furthermore, only stem bark extract (100 mg/mL) was able to inhibit the depletion of glutathione (13%). Discussion and conclusion: These results reinforce the therapeutic potential of stem bark and flower extracts of V. cauliflora to heal topical skin disease, namely in the treatment of neutrophil-related dermatosis and skin conditions related to oxidative stress, including skin aging.

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Protective effect of Ugni molinae Turcz against oxidative damage of human erythrocytes

Cited by 67 publications

References 24 publications

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Phenolic compounds and antioxidant activity of hydroalcoholic extracts of wild and cultivated murtilla (Ugni molinae Turcz.)

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

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