Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation

Halıcı, Zekai; Karaca, Mehmet; Keleş, Osman Nuri; Borekci, Bunyamin; Odabaşoğlu, Fehmi; Süleyman, Halis; Çadırcı, Elif; Bayır, Yasin; Ünal, Bünyami

doi:10.1016/j.fertnstert.2007.10.007

Cited by 81 publications

(78 citation statements)

References 35 publications

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“…Pretreatment with amlodipine markedly reduced the MDA content as compared to the I/R group, suggesting that amlodipine inhibits lipid peroxidation and protects the tissue against free radical damage. These findings are in agreement with previously published studies concluding that amlodipine reduces oxidative stress and protects against lipid peroxidation in cell membranes, a likely contributor to the cell death observed after I/R [13]. GSH plays a principal role in the cellular, protective system against toxic, free radical-induced tissue injury [31], and depletion of GSH increases the chance of free radical-induced damage [32].…”

Section: Discussionsupporting

confidence: 92%

“…In previous studies, we demonstrated that amlodipine exerts anti-inflammatory, anti-osteoporotic, and protective effects against ovarian ischemia-reperfusion (I/R) damage [12,13]. Amlodipine has also been clinically and experimentally shown to have antiatherogenic effects because it decreases and/or blocks inflammation from forming in the atheroma plaques on the vascular endothelium by inhibiting nitric oxide synthase activity [14].…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

Coşkun¹,

Günal²,

Halıcı³

et al. 2011

EAJM

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Objective: The aim of this study was to determine the potential, protective effects of amlodipine in an experimental, ischemia-reperfusion (I/R) model in the rabbit small intestine. Materials and Methods:The rabbits were divided into four groups: sham-operated, amlodipine (10 mg/kg) + sham-operated, I/R, and I/R + amlodipine (10 mg/kg) groups. An intestinal I/R model was applied to the rabbits. The superior mesenteric artery was occluded for 1 h with an atraumatic vascular clamp and then was reperfused for 2 h. Animals in the amlodipine and I/R + amlodipine groups received the amlodipine by oral gavage. At the end of the 2-h-reperfusion period, the animals were sacrificed.Results: Pretreatment with amlodipine significantly increased SOD activity and GSH levels to values close to those found in the serum from the I/R group. Rabbits in the I/R group showed high levels of serum MDA. Amlodipine pretreatment significantly reduced the serum MDA levels compared to the I/R group, although the MDA levels in the I/R + amlodipine group were still higher than in the sham-operated group. The I/R damage was ameliorated by amlodipine pretreatment, as evidenced by histopathological analysis. Conclusion:The present study is the first to report an attenuation of I/Rinduced intestinal injury by the systemic administration of amlodipine.Key Words: Amlodipine, Ischemia-Reperfusion, Glutathione, Superoxide Dismutase, Malonyldialdehyde, Rabbit Özet Amaç: Biz bu çalışmada, tavşan ince barsak iskemi-reperfüzyon (I/R) deneysel modeli üzerine amlodipin'in koruyucu etkisini inceledik. Gereç ve Yöntem:Tavşanlar dört guruba ayrıldı: şam operasyon gurubu, 10mg/kg amlodipin+şam operasyon gurubu, I/R gurubu, I/R+ 10 mg/kg amlodipin gurubu. İntestinal iskemi/reperfüzyon modeli tavşanlara uygulanıldı. Superior mezenterik arter 1 saat sure ile atravmatik vasküler klemp ile klempe edildi sonrasında 2 saat reperfüzyon uygulanıldı. Amlodipin ve amlodipin+I/R guruplarına oral gavaj ile amlodipin verildi. İki saatlik reperfüzyon süresinin sonunda hayvanlar sakrifiye edildi. I/R hasarının amlodipin uygulaması ile düzeldiği histopatolojik analizler ile ispatlandı. Bulgular: Amlodipin uygulaması I/R 'da serumda bulunan SOD aktivitesi ve GSH seviyesini anlamlı şekilde artırdı. Tavşanlarda intestinal I/R'ında yüksek seviyede MDA gözlendi. Amlodipin uygulaması intestinal I/R bulunan tavşan serumunda MDA aktivite seviyesini anlamlı şekilde düşürürken bu düşüş I/R+amlodipin gurubu control gurubu ile mukayese edildiğinde çok daha fazlaydı. Sonuç:Mevcut çalışma I/R ile oluşan intestinal hasar üzerine amlodipin'in sistemik uygulamasının iyleştirici etkisini gösteren ilk çalışmadır.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

Coşkun¹,

Günal²,

Halıcı³

et al. 2011

EAJM

Self Cite

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“…They include vascular congestion, atretic follicles, haemorrhage, edema and inflammatory cells infiltration (Coskun, et al, 2007;Guven et al, 2008;Halici et al, 2008). In this study, although not all the classical features of oxidativestress induced histopathological changes were observed however, it cannot be completely excluded that the ovary may be under some form of oxidative stress.…”

Section: Discussionmentioning

confidence: 52%

The effect of amodiaquine on oestrus cycle, ovarian histology and oxidative stress makers in regular cyclic sprague-dawley rats

Gbotolorun¹,

Osinubi²

2011

ABJNA

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This study investigated the effect of Amodiaquine hydrochloride (AQ.HCl) administered by gavage on the oestrous cycle, ovarian morphology and on the antioxidant status of superoxide dismutase (SOD) and catalase (CAT) in the ovary of 15 regular cyclic Sprague-Dawley rats. The experiment was divided into 3 groups as follows: group I-received 6 mg/kg bw AQ.HCl for 28 day; group II-received 12 mg/kg bw of AQ.HCl for 28 days; and group III-received distilled water and served as control. The animals were autopsied on the 28 th day by cervical dislocation. Result showed that the mean length of the oestrous cycle was prolonged in all the AQ.HCl treated rats when compared to the control. The increase in mean cycle length was statistically significant (p < 0.05) in the group that received 12 mg/kg bw of AQ.HCl. Histological sections of the ovaries of the rats treated with AQ.HCl revealed that the most remarkable change was widespread follicular atresia when compared to the control. The study revealed a reduction in the antioxidant status of SOD and CAT in the ovary. This reduction in anti-oxidant status was statistically significant (p < 0.05) for CAT. The result of this study showed that AQ.HCl is deleterious to the ovary by prolonging the length of the oestrous cycle, producing widespread follicular atresia and depletion of the enzymatic antioxidant status of SOD and CAT and consequently resulting in a state of oxidative stress in the ovary.

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“…Hypoxia generally impairs intracellular Ca +2 homeostasis and leads to various pathological situations such as neuronal cell damage, neurodegeneration, and cell death. The damage that occurs varies as a function of the duration of hypoxia as a result of ischemia according to fetal age [14][15][16]. We demonstrated that the survivals rates of infant rats born to rats with torsio/detorsio uteri were decreased.…”

Section: Metagenomic Sequencingmentioning

confidence: 83%

Clinical and Pathological Changes in the Offspring of Rats with Torsio Uteri

Rişvanlı¹,

Timurkaan²,

Saat³

et al. 2017

Journal of Veterinary Science and Animal Husbandry

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Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation

Abstract: Conservative treatment with amlodipine is effective in reducing tissue damage induced by ischemia, I/R, or both in ovaries.

Cited by 81 publications

References 35 publications

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

The effect of amodiaquine on oestrus cycle, ovarian histology and oxidative stress makers in regular cyclic sprague-dawley rats

Clinical and Pathological Changes in the Offspring of Rats with Torsio Uteri

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