The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

Coşkun, Ali; Günal, Armağan; Halıcı, Zekai; Oral, Akgün; Seyrek, Melik; Bayır, Yasin; Kılıç, Cenk; Yiğit, Taner; Özer, Tahir; Uzar, Ali İhsan

doi:10.5152/eajm.2011.07

Cited by 11 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24,25 Coskun et al demonstrated that AML treatment decreased MDA levels. 41 In the present study, MDA levels were increased in the P-induced toxicity groups, and the treatment with AML significantly reduced these levels.…”

Section: Groupsupporting

confidence: 54%

Protective effect of an L-type calcium channel blocker, amlodipine, on paracetamol-induced hepatotoxicity in rats

et al. 2018

View full text Add to dashboard Cite

Paracetamol (P), one of the most popular and commonly used analgesic and antipyretic agents, causes hepatotoxicity in overdoses. Amlodipine (AML), an L-type calcium channel blocker, has been shown to have anti-inflammatory activity by reversing the effect of calcium in the inflammation pathogenesis. In this study, the hepatoprotective activity of AML on P-induced hepatotoxicity was evaluated. Thirty male albino Wistar rats were divided into five groups: (1) control, (2) 2 g/kg of P, (3) 2 g/kg of P + 5 mg/kg of AML, (4) 2 g/kg of P + 10 mg/kg of AML, and (5) 10 mg/kg of AML. Some liver enzymes, oxidative parameters, cytokine mRNA expressions, histopathology, and immunohistochemical studies were performed in liver and blood samples. The serum levels of alanine aminotransferase and aspartate aminotransferase and the mRNA expression of tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta in the liver tissues were significantly increased in the group treated with P. The superoxide dismutase and glutathione parameters decreased and malondialdehyde levels increased in the livers of the rats treated with P. All these parameters were increased with both doses of the AML similar to the control group. A histopathological examination of the liver showed that AML administration ameliorated the P-induced inflammatory liver damage. In immunohistochemical staining, the expression of TNF-α in the cytoplasm of the hepatocytes was increased in the P group but not in other treatment groups when compared to the control. In conclusion, AML treatment showed significant protective effects against P-induced hepatotoxicity by increasing the activity of antioxidants and reducing inflammatory cytokines.

show abstract

Section: Groupsupporting

confidence: 54%

Protective effect of an L-type calcium channel blocker, amlodipine, on paracetamol-induced hepatotoxicity in rats

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Following ischemia lead to severe injury not only in ovaries but also in the different tissues 36,37 . It was demonstrated that antioxidant activity is important for repair of tissue damage 38 .…”

Section: Histopathological Resultsmentioning

confidence: 99%

Investigation and Histopathological Evaluation of the Effects of Omeprazole on the Ischemia-Reperfusion Induced Oxidative Damage and DNA Mutation in Rat Ovarian Tissue

Demiryılmaz¹,

Cetin²,

Yapça³

et al. 2013

Asian J. Chem.

View full text Add to dashboard Cite

Ovarian ischemia is usually caused by ovarian torsion, which leads to the obstruction of the ovarian artery and the ovarian vein. Ovarian ischemia is an emergency with an indication for acute intervention 1,2 . Though it can be encountered at all ages in women, it is more frequently seen in the premenarche and in the reproductive period 3 . In the case of late diagnosis and clinical necrosis, ovariectomy is the treatment of choice 4 . However, in children, in order to preserve the ovaries, even in cases with severe impairment of the circulation, it is advised to reperfuse the ovaries by means of detorsion 5-7 .However, contrary to the expectations of tissue perfusion, the reperfusion aggravates the effects of the ischemic damage 8 . This phenomenon, known as reperfusion damage, is initiated by the formation of free oxygen radicals 9 . For this reason, free oxygen radicals are known as the mediators of the reperfusion damage 10 . The mediators of the reperfusion damage (the free oxygen radicals) oxidate the cell membrane lipids and cause In this study, the biochemical and histopathological effects of omeprazole on ischemia-reperfusion (I/R) induced oxidative damage in rat ovarian tissue were investigated. The moment that the animals remained motionless in supine position was considered the appropriate time to perform surgery. The ovaries of the rats were reached through a 2.0-2.5 cm long vertical incision in the lower abdomen. Subsequently, in the omeprazole (OIR) and the control groups (I/RC), a vascular clip was placed in the lower part of the right ovary (the part where the ovary is attached to the uterus) and ischemia was maintained for 3 h. (No ischemia was applied in the healthy group.) After this period, the vascular clip was removed in order to provide reperfusion for 2 h. Afterwards, all the animals were terminated by high dose-anesthesia, the ovaries were removed and histopathological and biochemical studies were performed. Omeprazole has an antioxidant effect and it can have a protective function in the oxidative damage induced by ischemia-reperfusion. We have found that omeprazole prevents oxidative damage due to ischemia-reperfusion injury in rat ovarian tissue.

show abstract

“…Coskun et al . () examined the effects of AML and concluded that GSH levels significantly increased in the intestinal I/R in rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of amlodipine on ischaemia/reperfusion injury in the rat testis

et al. 2015

View full text Add to dashboard Cite

The aim of this study was to examine the effects of amlodipine (AML) in rat testicular torsion/detorsion damage. In this study, rats were divided into eight groups: (i) sham; (ii) testicular ischaemia, 2 h of ischaemia; (iii) testicular ischaemia/reperfusion (I/R), 2 h of ischaemia followed by 2 h of reperfusion; (iv) ischaemia + AML (5 mg kg(-1)) administered 30 min before ischaemia; (v) ischaemia + AML (10 mg kg(-1)) administered 30 min before ischaemia; (vi) and (vii) I/R + AML (5 mg kg(-1)) and I/R + AML (10 mg kg(-1)) administered 1.5 h after the induction of ischaemia, respectively, and at the end of a 2-h ischaemia period and a 2-h reperfusion period applied; and (viii) sham + AML (10 mg kg(-1)). Significant decreases in levels of superoxide dismutase and glutathione were observed in ischaemia and reperfusion groups when compared with healthy controls. These antioxidant levels increased in AML groups while malondialdehyde levels significantly decreased. While increases in tumour necrosis factor-alpha and transforming growth factor-beta levels were found in the torsion and detorsion groups, significant decreases in the levels of these inflammatory cytokines were observed in the treatment groups. These results demonstrate that AML significantly produced protective effects on testis tissue damage that occurs in the torsion/detorsion model via biochemical, histopathological and molecular pathways.

show abstract

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

Cited by 11 publications

References 34 publications

Protective effect of an L-type calcium channel blocker, amlodipine, on paracetamol-induced hepatotoxicity in rats

Protective effect of an L-type calcium channel blocker, amlodipine, on paracetamol-induced hepatotoxicity in rats

Investigation and Histopathological Evaluation of the Effects of Omeprazole on the Ischemia-Reperfusion Induced Oxidative Damage and DNA Mutation in Rat Ovarian Tissue

Effects of amlodipine on ischaemia/reperfusion injury in the rat testis

Contact Info

Product

Resources

About