Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts

Abstract

Background: Myocardial Injury caused by ischemia-reperfusion leads to cardiac dysfunction, tissue injury and metabolic changes. The production of reactive oxygen species (ROS) and lipid peroxidation are accompanied by ischemia-reperfusion injury. Objectives: The aim of this study was to assess the cardio protective potential effects of crocin in comparison with vitamin E on antioxidant capacity in ischemia-reperfusion of isolated rat hearts. Materials and Methods: Seventy male Sprague-Dawley rats were randomly divided into seven groups, including: sham, control and experimental groups treated with different doses of crocin (10, 20 and 40 mg/kg) or vitamin E (100 mg/kg) and a combination of crocin (40 mg/kg) with vitamin E (100 mg/kg) that were administrated orally for 21 days. The heart was quickly excised, transferred to a Langendorff apparatus at constant pressure and subjected to 30 minutes of global ischemia followed by 60 minutes of reperfusion. Cardiac damage markers and antioxidant enzymes were measured. Results:The results showed that superoxide dismutase and catalase enzyme activities increased and Mallon de aldehyde (MDA) decreased in animals pretreated by crocin (40 mg/kg) and vitamin E (100 mg/kg). Moreover, there was a significant improvement in post ischemic recovery of antioxidant capacity during reperfusion in rats receiving a combination of crocin (40 mg/kg) and vitamin E (100 mg/kg). Conclusions:The results demonstrated the protective role of crocin on antioxidant capacity, which may partially be related to stability or amplification of antioxidant systems. Like vitamin E, crocin may be beneficial for prevention or treatment of cardiac dysfunction and myocardial infarction in patients with ischemic heart disease.

Crocin treatment prevents doxorubicin-induced cardiotoxicity in rats

“…CRO treatment significantly improved left ventricular dysfunction induced by isoproterenol in rats [19]. Moreover, the protective role of CRO on antioxidant capacity in comparison with vitamin E has been observed, suggesting that CRO may be beneficial for preventing and treating the cardiac dysfunction and myocardial infarction in patients with ischemic heart disease [10].…”

Crocin Abrogates Carbon Tetrachloride-Induced Renal Toxicity in Rats via Modulation of Metabolizing Enzymes and Diminution of Oxidative Stress, Apoptosis, and Inflammatory Cytokines

“…Moreover, crocin has anticancer and apoptogenic effect on different human cancer cell lines and on animal xenograft . In addition, crocin exhibited free radical scavenging activity, antioxidant effect, and lipid peroxidation inhibition property .…”

“…Since oxidative stress is associated with CCl 4 ‐induced kidney toxicity, therefore, the use of antioxidants represents a comprehensive approach for protecting kidney. This approach is extremely popular and has been suggested as an effective therapeutic option for the protection and even treatment of many chemical‐induced organs damage .…”

“…Noteworthily, crocin has been utilized as protective agent against many chemicals‐induced organ toxicity including liver and brain . In addition, Naghizadeh et al.…”

Picroside II inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis by ameliorating mitochondrial function through a mechanism involving a decrease in reactive oxygen species production

“…Myocardial ischemia/reperfusion (I/R) injury refers to cardiac dysfunction induced by the restoration of blood flow following a period of ischemia or lack of oxygen (1). Myocardial I/R injury is a common clinical problem and frequently found in the process of coronary thrombolysis, coronary artery bypass graft and heart transplantation (2)(3)(4).…”