Protective effects of EGCG through Inhibition of NADPH oxidase expression in endothelial cells

Abstract

Green tea extract and epigallocatechin gallate (EGCG) have been extensively used in functional foods because of their beneficial health effects. In this study, the mechanism responsible for EGCG-induced protective effects in endothelial cells was investigated. EGCG exposure significantly decreased mRNA expression of the inflammatory genes monocyte chemotactic protein-1 (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1), and of the NADPH oxidase subunits p22phox and Nox2. In addition, EGCG reduced tumor necrosis factor (TNF)-α-induced p22phox mRNA expression. Results suggest that EGCG decreases oxidative stress and inflammation in cells via the reduced expression of NADPH oxidase, MCP-1, and VCAM-1 at the transcriptional level.

Laminin Receptor-Avid Nanotherapeutic EGCg-AuNPs as a Potential Alternative Therapeutic Approach to Prevent Restenosis

“…A number of additional studies have also reported that EGCg and related polyphenols present in green tea affects SMCs proliferation via activating p53, by down regulating NF-κB, or by suppressing mitogen activated protein kinase pathway [ 63 , 64 ]. A study reported in the literature depicts that EGCg decreases oxidative stress and inflammation in ECs by reducing NADPH oxidase expression at the transcriptional level [ 65 ]. Our findings corroborate earlier observations reported by Han and co-workers [ 66 ], related to the effects of EGCg on ECs and SMCs migrations.…”