Protective effects of<i>Stevia rebaudiana</i>aqueous extract on experimental unilateral testicular ischaemia/reperfusion injury in rats

Ganjiani, Vahid; Ahmadi, Nasrollah; Jahromi, Alireza Raayat

doi:10.1111/and.13469

Cited by 12 publications

(11 citation statements)

References 34 publications

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“…Despite effective surgical mediation, testicular atrophy and infertility rate increased between 40% and 60% (Bozkurt et al, 2019). In addition to testicular atrophy and infertility, reduction in hormone production is another major complication (Ganjiani et al, 2020). During the torsion period and resultant ischemia, ROS is assembled in the testicular tissue, while after surgery and torsion correction, reperfusion injury occurred due to reoxygenation of blood reopening (Gökçe et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Anaesthetics and citral were injected intraperitoneally with head down position in the lower left abdominal quadrant. Rats were placed in the dorsal recumbent position after anaesthesia induction (ketamine [Alfasan, The Netherlands, 80 mg/kg], xylazine [Alfasan, The Netherlands, 3 mg/kg], and midazolam [Exir Pharmaceutical Co., Iran, 3 mg/kg]) (Ganjiani et al, 2020). Immediately after anaesthesia induction, the surgical area was shaved and disinfected.…”

Section: Methodsmentioning

confidence: 99%

“…• Group V (intact group) only received citral (300 mg/kg) as much as a reperfusion period (24 h) to determine any possible side effects of citral on testicular tissue. Co., Iran, 3 mg/kg]) (Ganjiani et al, 2020). Immediately after anaesthesia induction, the surgical area was shaved and disinfected.…”

Section: Animals Ethics and Experimental Designmentioning

confidence: 99%

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The evaluation of citral effects on experimental unilateral testicular ischemia/reperfusion injury

et al. 2022

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This investigation aimed to evaluate the defensive impacts of citral on ischemia/reperfusion (I/R) injury induced by testicular torsion/detorsion (T/D) in rats in an experimental model. The grouping of subjects was as follows: (1) sham, (2) T/D, (3) and (4) T/D plus citral 150 and 300 mg/kg, respectively, and (5) intact (citral 300 mg/kg). T/D was performed by testicular 720° turning for 2 h and then detorsion for 24 h. Blood serum was obtained to assess testosterone and oxidative stress markers, epididymal sperms were collected for sperm staining and sperm analysis, and testicular tissues were examined for histopathology. T/D damage was associated with a remarkable decline in sperm total count, viability, and some velocity parameters in comparison to the sham group (p < 0.05), which could be reversed significantly by citral (p < 0.05). Histopathologically, T/D damage caused severe oedema, haemorrhage, inflammation, and seminiferous tubules disruption, while citral improved significantly the mean seminiferous tubular diameter, Cosentino's score, and Johnsen's score (p < 0.05). I/R injury was associated with significant increased malondialdehyde and oxidative stress index, and also significant reduced total antioxidant capacity and testosterone versus the sham group (p < 0.05), which all were prevented significantly by citral administration (p < 0.05). The outcomes greatly proved that testicular I/R injury could be significantly prevented by citral.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The evaluation of citral effects on experimental unilateral testicular ischemia/reperfusion injury

et al. 2022

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“…However, our study could not be detailed by hormone analyses and immune staining techniques. Testicular torsion is an urgent urological problem known since the 1800s with irreparable risks such as testicular atrophy, subfertility, fertility and endocrine disorders (Ganjiani et al., 2020). Several previously conducted clinical trials have reported that more than half of patients diagnosed with testicular torsion are under the age of 21 (Cumming et al., 2002).…”

Section: Discussionmentioning

confidence: 99%

Milrinone ameliorates ischaemia‐reperfusion injury in experimental testicular torsion/detorsion rat model

et al. 2021

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This experimental study aims to evaluate the efficacy of milrinone against ischaemia‐reperfusion injury due to testicular torsion/detorsion. Group 1 was defined as the control group. Testicular torsion/detorsion model was performed in Group 2. Group 3 had similar procedures to the rats in Group 2. In addition, 0.5 mg/kg of milrinone was administered intraperitoneally immediately after testicular torsion in Group 3. Histopathological examinations indicated a dramatic improvement in terms of inflammation, haemorrhage, oedema, congestion, Cosentino and Johnson scores in Group 3 compared to Group 2 (p = .037, p = .045, p = .018, p = .040, p = .033 and p = .03 respectively). Blood biochemical analyses, superoxide dismutase (SOD), glutathione peroxidase (GSH‐px) activity and total antioxidant status (TAS) levels increased significantly in Group 3 compared to Group 2 (p = .001, p = .024 and p < .001). Malondialdehyde (MDA), protein carbonyl (PC), interleukin 1beta (IL‐1beta), tumour necrosis factor‐alpha (TNF‐alpha) and total oxidant status (TOS) levels decreased in Group 3 compared to Group 2 (p = .001, p = .018, p < .001, p = .036 and p = .002 respectively). Tissue biochemical analyses determined an increase in SOD and GSH‐px activity in Group 3 compared to Group 2, while PC and MDA levels were reduced (p = .001, p < .001, p = .038 and p < .001 respectively). Milrinone attenuates ischaemia‐reperfusion injury that causes highly harmful effects due to testicular torsion/detorsion.

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“…Stevia group: For 56 days, Stevia was allowed to dissolve in water (1000 mg/kg of body weight). 17 Tartrazine group: were administrated orally by gavage Tartrazine dissolved in water (300 mg/kg body weight) for 56 days. 1,3 Stevia and Tartrazine group: were administrated orally by gavage Tartrazine after 1 h of administrating Stevia for 56 days.…”

Section: Study Design and Animalsmentioning

confidence: 99%

The protective effect of aqueous extract ofStevia rebaudianaagainst tartrazine toxicity in male Wistar rat

Hanna,

Beshay,

El. Shafee

et al. 2023

Cell Biochemistry & Function

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Tartrazine is a yellow colouring agent that is commonly used in foods; however, high dosages of Tartrazine affect fertility and create oxidative stress by generating free radicals. A plant species known as Stevia rebaudiana has natural antioxidants that show promise for protecting testicular tissue. Consequently, this study was intended to examine the ameliorative effect of the aqueous extract of S. rebaudiana (Stevia) on the fertility of male Wistar rats induced by the daily oral intake of Tartrazine. Utilizing gas chromatography–mass spectrometry, phytochemical identification was accomplished for Stevia extract. Study groups were separated into several groups: the first group (the control) got distilled water for up to 56 days; the Stevia group (1000 mg/kg), the Tartrazine group (300 mg/kg) and the Stevia and Tartrazine group (the group was given Tartrazine after 1 h of Stevia extract intake). Also, the oxidative damage in testicular tissues was assessed by measuring the levels of malondialdehyde (MDA) and antioxidants (catalase [CAT], superoxide dismutase [SOD] and glutathione reductase [GSH]). Further, histological alterations were examined. In addition, cyclic AMP‐responsive element modulator (Crem) gene expression levels and their relative proteins were measured in the testicular tissues using quantitative real‐time polymerase chain reaction and enzyme‐linked immunosorbent assays, respectively. Sperm analysis and testosterone concentration were also performed. SPSS version 25 was used for the analysis of results while (p < .05) was regarded as significant. Compared with the control group, the results demonstrated that Tartrazine caused a significant reduction (p < .05) in the testosterone hormone level (0.70 ± 0.21) and the Crem protein quantity (1.21 ± 0.23) in the treated Tartrazine group. Also, it had a significant decrease (p < .05) in sperm motility, viability, count and antioxidant levels. Moreover, there was a significant increase (p < .05) in sperm abnormalities, MDA level (7.40 ± 1.10), kidney and liver function parameters, and DNA degradation in the treated Tartrazine group compared with the control group. On the contrary, the Stevia extract intake enhanced the testosterone (2.50 ± 0.60), antioxidants and Crem protein levels (2.33 ± 0.10) with an improvement in sperm quality in the Stevia and Tartrazine‐treated group compared with the Tartrazine group. Stevia also caused a significant decrease (p < .05) in the MDA level (3.20 ± 0.20), and sperm abnormalities with an enhancement of the liver and kidney function parameters in the Stevia and Tartrazine‐treated group compared to the Tartrazine group. Stevia administration has a protective effect on the testicular tissues and sperm quality against toxicity induced by Tartrazine exposure, so it will be a good antioxidant drug to be administered daily before daily administration of Tartrazine.

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Protective effects ofStevia rebaudianaaqueous extract on experimental unilateral testicular ischaemia/reperfusion injury in rats

Cited by 12 publications

References 34 publications

The evaluation of citral effects on experimental unilateral testicular ischemia/reperfusion injury

The evaluation of citral effects on experimental unilateral testicular ischemia/reperfusion injury

Milrinone ameliorates ischaemia‐reperfusion injury in experimental testicular torsion/detorsion rat model

The protective effect of aqueous extract ofStevia rebaudianaagainst tartrazine toxicity in male Wistar rat

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