Protective Effects of<i>Tinospora crispa</i>Stem Extract on Renal Damage and Hemolysis during<i>Plasmodium berghei</i>Infection in Mice

Nutham, Narain; Sakulmettatham, Sakuna; Klongthalay, Suwit; Chutoam, Palatip; Somsak, Voravuth

doi:10.1155/2015/738608

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…The observed potential therapeutic effect of the extracts on liver enzyme activity could be attributed to parasite elimination and/or antioxidant effects of the plant extracts, which could be derived from its bioactive constituents (Gomes et al, 2011;Nutham et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective activity of Eucalyptus camaldulensis extract in murine malaria mediated by suppression of oxidative and inflammatory processes

Aljawdah

Abdel‐Gaber

Al-Shaebi

et al. 2022

Front. Cell. Infect. Microbiol.

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Herbal extracts are promising agents against various parasitic diseases, such as malaria. This study aimed to evaluate the ameliorative action of Eucalyptus camaldulensis extract (ECE) against hepatic damage caused by Plasmodium chabaudi infection. Mice were allocated into five groups as follows: two groups served as the control non-infected groups that received distilled water and ECE, respectively; subsequent three groups were infected with 106 P. chabaudi parasitized erythrocytes; the last two groups were infected with the parasite and then treated with ECE and chloroquine. On day 8 post-infection, the parasite count increased inside erythrocytes (59.4% parasitemia in the infected group). Parasitemia was successfully reduced to 9.4% upon ECE treatment. Phytochemical screening using GC mass spectrometry revealed that ECE contained 23 phytochemical components. Total phenolics and flavonoids in ECE were 104 ± 2 and 7.1± 3 µg/mL, respectively, with 57.2% antioxidant activity. ECE ameliorated changes in liver histopathology and enzymatic activity of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase. In addition, ECE prevented oxidative damage induced by the parasite in the liver, as evidenced by the change in the liver concentrations of glutathione, nitric oxide, malondialdehyde, and catalase. Moreover, ECE was able to regulate the expression of liver cytokines, interleukins-1β and 6, as well as IFN-γ mRNA. ECE possesses antiplasmodial, antioxidant, and anti-inflammatory activity against liver injury induced by the parasite P. chabaudi.

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Section: Discussionmentioning

confidence: 99%

Hepatoprotective activity of Eucalyptus camaldulensis extract in murine malaria mediated by suppression of oxidative and inflammatory processes

Aljawdah

Abdel‐Gaber

Al-Shaebi

et al. 2022

Front. Cell. Infect. Microbiol.

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“…For extraction, the powdered plant material was extracted with distilled water at the ratio of 1 : 10 using a microwave-assisted method [10, 11] and incubated at room temperature for 3 consecutive days with occasional stirring. After that, filtration was carried out with the Whatman no.…”

Section: Methodsmentioning

confidence: 99%

Antimalarial Activity of Tinospora baenzigeri against Plasmodium berghei-Infected Mice

Ounjaijean

Kotepui

Somsak

2019

Journal of Tropical Medicine

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Plant species of the genus Tinospora (Menispermaceae) possess several pharmacological properties, and T. crispa has been reported to have antimalarial activity. T. baenzigeri (Chingcha Chalee) is a rich source of terpenes and quinoline alkaloids; however, it still has not yet been investigated the antimalarial activity of this plant extract. Hence, this study was aimed to evaluate the antimalarial activity of T. baenzigeri stem extract against Plasmodium berghei-infected mice. The aqueous crude extract of T. baenzigeri stem was prepared using a microwave-assisted method and tested for acute toxicity in mice. For evaluating the antimalarial activity in vivo, the standard 4-day test was carried out using groups of ICR mice infected with P. berghei ANKA administered orally by gavage with the extract (100, 250, and 500 mg/kg) for 4 consecutive days. Parasitemia, body weight, packed cell volume, and mean survival time were then measured. It was found that the aqueous crude extract of T. baenzigeri stem did not exhibit any sign of toxicity up to the dose of 2,000 mg/kg. The extract significantly (P<0.01) inhibited parasitemia in a dose-dependent manner, with 22.02%, 50.81%, and 74.95% inhibition. Moreover, the marked prevention of body weight loss and packed cell volume reduction was observed at doses of 100, 250, and 500 mg/kg of extract-treated mice. Additionally, the extract prolonged the mean survival time of P. berghei-infected mice, compared to the untreated group. In conclusion, the aqueous crude extract of T. baenzigeri stem has demonstrated potent antimalarial activity against P. berghei-infected mice with prolonged mean survival time and prevention of body weight loss and packed cell volume reduction.

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“…to mice, showed signs of behavioral toxicity. They also reported that the aqueous extract at the dose of 500 mg/kg exerted a hepatoprotective effect during Plasmodium berghei infection in mice [10]. However, in another study, the ethanolic extract of the dried stems of T. crispa (100 and 200 mg/ kg/day for 8 wk oral) was shown to potentiate thioacetamide-induced hepatotoxicity [15].…”

Section: Introductionmentioning

confidence: 97%

“…The stems have been reported to possess antidiabetic activity [7][8][9], while its traditional use encompasses treatment of a wide spectrum of medical conditions. Although there are several reports that endorse the medicinal significance of T. crispa [10][11][12][13], several preclinical and clinical reports illustrate its toxicity. While no reports exist on the LD 50 of T. crispa in laboratory animals, the crude extract of T. crispa has been studied for its hepatotoxic potential in rats and mice.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the hepatotoxic potential of Tinospora crispa and its isolated borapetosides B, C and F in a murine model

et al. 2020

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The hepatotoxic potential of the methanolic extract of the stems of Tinospora crispa and of its furano-diterpenoid–borapetosides B, C, and F–was investigated in normal and health-compromised mice. Health-compromised condition was established by a single intraperitoneal injection of LPS (6 mg/kg). Two different sets of experiments were conducted to evaluate the hepatotoxic potential. A 21-day study where the mice were dosed with the extract of T. crispa (1 gm/kg b. wt./day) or standardized combination of borapetosides B, C and F (500 mg/kg b. wt.) with or without a single dose of LPS (1 gm/kg b. wt./day). In the acute toxicity study, mice were dosed with borapetosides B, C, and F (500 mg/kg b. wt.). Results showed that the ALT levels were normal and liver histopathology unaltered. No conclusive hepatotoxicity was observed in the methanolic extract or pure compounds tested under the given experimental conditions.

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Protective Effects ofTinospora crispaStem Extract on Renal Damage and Hemolysis duringPlasmodium bergheiInfection in Mice

Cited by 14 publications

References 27 publications

Hepatoprotective activity of Eucalyptus camaldulensis extract in murine malaria mediated by suppression of oxidative and inflammatory processes

Hepatoprotective activity of Eucalyptus camaldulensis extract in murine malaria mediated by suppression of oxidative and inflammatory processes

Antimalarial Activity of Tinospora baenzigeri against Plasmodium berghei-Infected Mice

Evaluation of the hepatotoxic potential of Tinospora crispa and its isolated borapetosides B, C and F in a murine model

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