Protective Effects of Lactoferrin against Intestinal Mucosal Damage Induced by Lipopolysaccharide in Human Intestinal Caco-2 Cells

Hirotani, Yoshihiko; Ikeda, Kenji; Kato, Ryuichi; Myotoku, Michiaki; Umeda, Takashi; Ijiri, Yoshio; Tanaka, Kazuhiko

doi:10.1248/yakushi.128.1363

Cited by 69 publications

(61 citation statements)

References 31 publications

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“…24 Multiple components in human milk may be responsible for these protective effects. 97 Lactoferrin has been shown to reduce increased epithelial permeability caused by lipopolysaccharide in vitro, 98 and whey protein and transforming growth factor-b (TGF-b) decrease intestinal permeability by upregulating claudin-4 expression. 99 In addition, casein improves intestinal barrier function by upregulating claudin-1 expression while decreasing claudin-2 expression.…”

Section: Development Of the Intestinal Epithelial Barriermentioning

confidence: 99%

The role of intestinal epithelial barrier function in the development of NEC

2015

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The intestinal epithelial barrier plays an important role in maintaining host health. Breakdown of intestinal barrier function is known to play a role in many diseases such as infectious enteritis, idiopathic inflammatory bowel disease, and neonatal inflammatory bowel diseases. Recently, increasing research has demonstrated the importance of understanding how intestinal epithelial barrier function develops in the premature neonate in order to develop strategies to promote its maturation. Optimizing intestinal barrier function is thought to be key to preventing neonatal inflammatory bowel diseases such as necrotizing enterocolitis. In this review, we will first summarize the key components of the intestinal epithelial barrier, what is known about its development, and how this may explain NEC pathogenesis. Finally, we will review what therapeutic strategies may be used to promote optimal development of neonatal intestinal barrier function in order to reduce the incidence and severity of NEC.

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Section: Development Of the Intestinal Epithelial Barriermentioning

confidence: 99%

The role of intestinal epithelial barrier function in the development of NEC

2015

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“…As suggested by in vitro assays, these protective effects against damage to the intestinal mucosa could be based on the capacity of Lf to attenuate the increase in epithelial permeability caused by LPS [60].…”

Section: Neutralizing Effects Of Lf On the Lps-induced Inflammatory Rmentioning

confidence: 99%

Lactoferrin-lipopolysaccharide (LPS) binding as key to antibacterial and antiendotoxic effects

Drago-Serrano

Garza-Amaya

Luna

et al. 2012

International Immunopharmacology

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“…It is reported that the polarized Caco-2 cell layer shows differential responses to LPS challenge depending on the side to which it was applied. 24, 40–42 LPS added to the basolateral side of Caco-2 cell layer at a concentration greater than 0.3 ng/mL causes an increase in the permeability of Caco-2 cell monolayer 41 due to the activation of TLRs on the basolateral surface. 41, 43, 44 On the other hand, the apical side remains resistant to much higher concentrations of LPS (20–50 μg/mL) and does not change its permeability.…”

Section: Discussionmentioning

confidence: 99%

“…23, 24 After LPS and/or ZWC treatment, the medium was replaced with fresh medium, and TEER was measured for 96 h from the initiation of each treatment.…”

Section: Methodsmentioning

confidence: 99%

Succinylated Chitosan Derivative Has Local Protective Effects on Intestinal Inflammation

Hyun

Hashimoto-Hill

Kim

et al. 2017

ACS Biomater. Sci. Eng.

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We have previously reported on the anti-inflammatory effects of a water-soluble chitosan derivative, zwitterionic chitosan (ZWC). In the present study, we hypothesized that orally-administered ZWC would provide local anti-inflammatory effects in the intestinal lumen. ZWC indeed showed anti-inflammatory effects in various in-vitro models including peritoneal macrophages, engineered THP1 monocytes, and Caco-2 cells. In Caco-2 cells, ZWC applied before the lipopolysaccharide (LPS) challenge was more effective than when it was applied after it in preventing LPS-induced cell damage. When administered to mice via drinking water as a prophylactic measure, ZWC protected the animals from 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis, helping them to recover the body weight, restore the gross and histological appearance of the colon, and generate FoxP3+ T cells. In contrast, orally-administered ZWC did not protect the animals from LPS-induced systemic inflammation. These results indicate that orally-administered ZWC reaches the colon with minimal absorption through the upper gastrointestinal tract and provides a local anti-inflammatory effect.

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Protective Effects of Lactoferrin against Intestinal Mucosal Damage Induced by Lipopolysaccharide in Human Intestinal Caco-2 Cells

Cited by 69 publications

References 31 publications

The role of intestinal epithelial barrier function in the development of NEC

The role of intestinal epithelial barrier function in the development of NEC

Lactoferrin-lipopolysaccharide (LPS) binding as key to antibacterial and antiendotoxic effects

Succinylated Chitosan Derivative Has Local Protective Effects on Intestinal Inflammation

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