Protective Effects of Propolis and Probiotic Lactobacillus acidophilus against Carbon Tetrachloride-Induced Hepatotoxicity in Rats

Abstract

Background: Propolis (PRS) and probiotic bacteria Lactobacillus are natural products used as dietary supplement for their therapeutic benefits. This study was performed to examine the possible hepatoprotective effect of PRS and probiotics (PRCs) against carbon tetrachloride-induced liver injury. Methods: Experimentally, intoxicated rats received 0.5 ml/kg CCl4 (i.p.) daily for six days, pretreated rats received per os PRS 100 mg/kg or PRCs 109 CFU for six days followed by a single dose of 0.5 ml/kg CCl4. Control groups received either PRS, PRCs or olive oil for six days. Then, serum biochemistry (total protein, cholesterol, triglycerides and albumin) and oxidative stress parameters were measured. Results: We showed that CCl4 treatment was associated with an increase of the serum aspartate amino transferase (AST), alanine aminotransferase (ALT), cholesterol and triglycerides levels. In parallel, serum total protein, albumin and blood sugar levels were significantly decreased. Regarding the oxidative stress parameters, catalase and glutathione S-transferase (GST) levels were lower, conversely to the lipid peroxidation (MDA). Conclusion: Our results strongly support that administration of PRS and PRCs may significantly protect liver against CCl4-induced toxicity by enhancing antioxidative stress pathway and preventing lipid peroxidation.

Effect of Some Strains of Lactic Acid Bacteria Isolated From Some Fermented Dairy Products on Carbon Tetrachloride-Induced Hepatotoxicity and Nephrotoxicity of Albino Rats.

“…Results obtained in this study are in good agreement with what obtained by Oumeddour et al . ( 2019) who showed that treatment with CCl 4 resulted in a major de ciency in the liver catalase of the enzymatic antioxidant defense mechanism, Also Heeba and Mahmoud (2014) showed that no signi cantly increase when rats treated with CCl4.…”

“…Results obtained in this study are in the same line with Salatin et al (2019), andOumeddour et al (2019) they are also in accordance with Fahmy et al(2016) and Al-Sayed et al . ( 2015) stated that CCl 4 increases biochemical parameters such as AST, ALT, urea and creatinine serum levels.…”

“…, 1977). Oumeddour et al ( 2019) reported that rats were prevented from CCl 4 -induced hyperglycemia by the administration of probiotic bacteria. Even though the existence of bioactive components has been hypothesized to inhibit beta-cell apoptosis and increase insulin secretion (Al-Hariri, 2011).…”

Could probiotics protect against human toxicity caused by polystyrene nanoplastics and microplastics?

“…S. boulardii was able to improve the tissue antioxidant status and inhibit neutrophil recruitment, thereby reducing intestinal damage and associated hepatitis ( 134 ). A reduction of oxidative damage in hepatocytes by probiotics was also reported by Oumeddour et al ( 135 ).…”