Protective effects of tanshinone IIA on endothelial progenitor cells injured by tumor necrosis factor-α

Abstract

Tanshinone IIA (Tan IIA) is a Traditional Chinese Medicine commonly used in Asian and Western countries for the prevention and treatment of cardiovascular disorders, such as atherosclerosis. Endothelial dysfunction and associated inflammatory processes have a critical role in the development of atherosclerosis. Endothelial progenitor cells (EPCs) have been demonstrated to be involved in certain aspects of the endothelial repair process. The present study aimed to investigate the putative protective effects of Tan IIA on EPCs injured by tumor necrosis factor-α (TNF-α). The potential effects of Tan IIA on TNF-α-stimulated EPC proliferation, migration, adhesion, in vitro tube formation ability and paracrine activity were investigated in the current study. The results indicated that TNF-α impaired EPC proliferation, migration, adhesion capacity and vasculogenesis ability in vitro as well as promoted EPC secretion of inflammatory cytokines, including monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and soluble CD40 ligand (sCD40L). However, Tan IIA was able to reverse these effects. In conclusion, these findings demonstrated that Tan IIA may have the potential to protect EPCs against damage induced by TNF-α. Therefore, these results may provide evidence for the pharmacological basis of Tan IIA and its potential use in the prevention and treatment of early atherosclerosis associated with EPC and endothelial damage.

Salvia miltiorrhiza in Treating Cardiovascular Diseases: A Review on Its Pharmacological and Clinical Applications

“…TsIIA can affect high-density lipoprotein (HDL) subtractions distribution and the intake and efflux of cholesterol (Jia et al, 2016); inhibit endothelial progenitor cell (EPC) injury (Wang et al, 2015), apoptosis of VSMCs (Wang et al, 2017), proliferation and migration of macrophages (Wang et al, 2017), foam cell formation (Liu et al, 2014b), and vascular inflammation (Yang et al, 2016a; Wang et al, 2017; Xuan et al, 2017); and enhance vulnerable plaque stability (Li et al, 2015a; Zhao et al, 2016). These effects can inhibit the progression of AS.…”

Resveratrol prevents TNF-α-induced VCAM-1 and ICAM-1 upregulation in endothelial progenitor cells via reduction of NF-κB activation

“…Rat bone marrow-derived EPCs were cultured according to previously published methods. 18 , 19 In brief, MNCs from rat femurs were purified by density gradient centrifugation and cultured in EBM-2 medium (Lonza, Basel, Switzerland) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA). Rapidly adhering mature ECs and hematopoietic cells were removed by aspirating non-adherent cells and transferring them into new culture dishes after 24 and 48 hours.…”

Common Injuries and Repair Mechanisms in the Endothelial Lining

“…Finally, it is worth mentioning that the underlying influences of Tan II A on tumor necrosis factor (TNF)-α-motivated EPC proliferation, formation ability, and paracrine activity in vitro tubes, as demonstrated by Wang et al . [ 56 ] The results predicted that TNF-α damaged EPC proliferation competence and neovascularization capacity in vitro and boosted the EPC excretion of inflammation factors such as soluble CD40 ligand, monocyte chemoattractant protein-1, and IL-6. Nevertheless, these effects were able to be reversed by Tan II A.…”