Protective Effects of Unsaponifiable Matter from Perilla Seed Meal on UVB-induced Damages and the Underlying Mechanisms in Human Skin Fibroblasts

Abstract

Unsaponifiable matter (USM) from perilla seed meal contains numerous phytochemicals, including tocopherols, phytosterols, squalene, and policosanols, that exhibit antioxidant and health-promoting properties. In this study, the protective effects of USM on UVB-induced skin aging were investigated in Hs68 cells. UVB irradiation decreased cell viability by 26% compared to the control. However, USM blocked UVB-induced cytotoxicity. Moreover, USM treatment significantly decreased the UVB-induced production of reactive oxygen species and attenuated the UVB-induced production and mRNA expression of matrix metalloproteinases (MMPs) by inhibiting the phosphorylation of mitogen-activated protein kinases and activator protein 1 (AP-1). Furthermore, UVB exposure led to a 49.4% reduction in collagen synthesis. However, USM treatment restored collagen synthesis through upregulation of the transforming growth factor beta (TGF-β)/Smad2/3 pathways. These data indicate that USM regulates the production of MMPs and collagen by modulation of the TGF-β/Smad pathway and AP-1 activity, suggesting that USM may be a useful anti-photoaging ingredient.

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

“…The protective effects of UVB-induced damages were also examined using unsaponifiable matter (USM) from perilla seed meal [23]. Similar results detected in the present study were observed in USM-treated human skin fibroblasts (Hs68 cells) after UVB radiation although there are differences in the analysis factors.…”

“…Similar results detected in the present study were observed in USM-treated human skin fibroblasts (Hs68 cells) after UVB radiation although there are differences in the analysis factors. USM treatment at 1.0, 2.5 and 5.0 µg/mL suppressed UVB-induced cytotoxicity and ROS production in Hs68 cells [23]. Furthermore, decrease of total collagen production in UVB treated cells significantly recovered by USM treatment, while the opposite pattern observed on MMP1 and 3 secretion and mRNA under the same condition [23].…”

“…USM treatment at 1.0, 2.5 and 5.0 µg/mL suppressed UVB-induced cytotoxicity and ROS production in Hs68 cells [23]. Furthermore, decrease of total collagen production in UVB treated cells significantly recovered by USM treatment, while the opposite pattern observed on MMP1 and 3 secretion and mRNA under the same condition [23]. However, there is a difference in the composition between USM and CPO.…”

“…However, there is a difference in the composition between USM and CPO. USM contains high concentration of polycosanols, tocopherols, phytosterols and squalene [23].…”

Anti-Photoaging and Anti-Melanogenesis Effects of Fucoidan Isolated from Hizikia fusiforme and Its Underlying Mechanisms

“…Reactive oxygen species (ROS) play an important role in human health because they are related to various diseases. Abnormal ROS production leads to various adverse effects, including damage to essential macromolecules such as DNA, lipids, and proteins [ 30 , 31 , 32 , 33 , 34 ]. Accumulation of this molecular damage can subsequently cause cell apoptosis, necrosis, and death.…”