Protective role of HSP27 against UVC-induced cell death in human cells

Wano, Chieko; Kita, K; Takahashi, Shunji; Sugaya, Shigeru; Hino, Mizuki; Hosoya, Hiroshi; Suzuki, Nobuo

doi:10.1016/j.yexcr.2004.04.048

Cited by 32 publications

(50 citation statements)

References 39 publications

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“…Web site), similarly to the stable transfectants with HSP27 cDNA, as described previously. 4) To confirm the role of annexin II expression levels in the resistance to UVC-induced cell death, we established cells with stable overexpression of annexin II protein by transfection of RSa cells with annexin II cDNA. The transfected cells, ANXII-11 and ANXAII-13, showed higher levels of annexin II than the control RSa cells transfected with empty vector, V1 and V2 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Immunoblotting analysis was carried out as described previously. 4) In brief, cells were washed twice with phosphate-buffered saline and whole cells were lysed with SDS sampling buffer and boiled for 5 min. Whole cell lysates were separated by 12% SDS gel and transferred onto a Millipore membrane.…”

Section: Methodsmentioning

confidence: 99%

“…Full-length human HSP27 cDNA was prepared as described previously. 4) The cDNA was ligated into pQE-30 plasmid (Qiagen, Valencia, CA) using Sac I and Pst I restriction sites. The construction was confirmed by sequencing and was then digested with EcoR I and Hind III.…”

Section: Methodsmentioning

confidence: 99%

“…4) An important function of HSP27 is to act as a molecular chaperone. Under stressful conditions, HSP27 proteins form complexes with a variety of cellular proteins, such as Akt, 8) cytochrome c, 9) actin microfilament, 10) and eIF4G, 11) resulting in inhibition of apoptosis induction, 8,9) cytoskeleton disruption, 10) and translation initiation 11) respectively.…”

Section: ) Rsa Cells Transfected With Hsp27mentioning

confidence: 99%

“…[1][2][3] We have found that one HSP, HSP27, may be involved in the resistance of human cells to UVC-induced cell death. 4) This finding was based on a comparison of gene expression profiles using a cDNA array between human RSa cells and their variant AP r -1 cells. RSa cells were established from human embryo-derived fibroblastic cells by double infection with Simian virus 40 and Rous sarcoma virus.…”

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confidence: 99%

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The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1

Jin

Kita

Sun

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ) Rsa Cells Transfected With Hsp27mentioning

confidence: 99%

mentioning

confidence: 99%

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The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1

Jin

Kita

Sun

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Involvement of Heat Shock Proteins in Protection of Tumor Cells from Genotoxic Stresses

O'Callaghan-Sunol

Gabai

Heat Shock Proteins

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Abstract:Anti-apoptotic functions of heat shock proteins Hsp70 and Hsp27 are well established. However, radiation and genotoxic antineoplastic drugs at clinically relevant doses induce apoptosis mostly in lymphoid cells, while in epithelial tumors they evoke different type of response, mainly senescence and mitotic catastrophe, which leads to loss of clonogenic potential of cells. Here we review old and new data showing that upregulation of Hsp27 or Hsp70 levels protect various tumor cell lines from gamma-and UV-radiation and genotoxic anti-neoplastic drugs. Accordingly, downregulation of Hsp27 or Hsp70 levels by antisense or siRNA sensitizes tumor cells to these agents. Importantly, protection and sensitization by modulation of Hsp27 or Hsp70 levels were manifested not only by modulation of apoptosis, but by clonogenic survival as well, and recent data indicate that these Hsps can suppress also drug-induced senescence. Several studies demonstrated that intrinsic and acquired chemo-and radioresistance in tumor cell lines and in patients with certain forms of cancers can be associated with upregulation of Hsp27 and/or Hsp70. Possible mechanisms of Hsp-induced protection, in particular, modulation of p53-dependent and p53-independent DNA-damaging signaling pathways, are discussed

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Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells

Zhang

Liang

et al. 2012

J Cancer Res Clin Oncol

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Protective role of HSP27 against UVC-induced cell death in human cells

Cited by 32 publications

References 39 publications

The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1

The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1

Involvement of Heat Shock Proteins in Protection of Tumor Cells from Genotoxic Stresses

Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells

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Protective role of HSP27 against UVC-induced cell death in human cells

Cited by 32 publications

References 39 publications

The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and APr-1

The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and APr-1

Involvement of Heat Shock Proteins in Protection of Tumor Cells from Genotoxic Stresses

Identification of heat shock protein 27 as a radioresistance-related protein in nasopharyngeal carcinoma cells

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Product

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The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1

The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1