Protective role ofortho-substituted Mn(III)N-alkylpyridylporphyrins against the oxidative injury induced bytert-butylhydroperoxide

Abstract

The present work addresses the role of two ortho-substituted Mn(III) N-alkylpyridylporphyrins, alkyl being ethyl in MnTE-2-PyP(5+) and n-hexyl in MnTnHex-2-PyP(5+), on the protection against the oxidant tert-butylhydroperoxide (TBHP). Their protective role was studied in V79 cells using endpoints of cell viability (MTT and crystal violet assays), intracellular O(2)*- generation (dihydroethidium assay) and glutathione status (DTNB and monochlorobimane assays). MnPs per se did not show cytotoxicity (up to 25 microM, 24 h). The exposure to TBHP resulted in a significant decrease in cell viability and in an increase in the intracellular O(2)(*-) levels. Also, TBHP depleted total and reduced glutathione and increased GSSG. The two MnPs counteracted remarkably the effects of TBHP. Even at low concentrations, both MnPs were protective in terms of cell viability and abrogated the intracellular O(2)(*-) increase in a significant way. Also, they augmented markedly the total and reduced glutathione contents in TBHP-treated cells, highlighting the multiple mechanisms of protection of these SOD mimics, which at least in part may be ascribed to their electron-donating ability.

Cutaneous biocompatible rutin-loaded gelatin-based nanoparticles increase the SPF of the association of UVA and UVB filters

“…There was a significant difference between GNPs and GTA on the less concentrated samples. The ability of free GTA to crosslink structure of proteins by bridging free amino groups decreased HaCaT cells viability in a concentration-dependent manner, as well as, the association of GTA and GNPs increased the cell viability (Alarifi et al, 2013;Fernandes et al, 2010;UNITED STATES, 2014). Besides the correlation between irritant potential and reduced cell viability, there are other important confounding factors that must be clarified with in vivo safety assessments.…”

“…DMSO (20% v/v) and Milli-Q water (5% v/v) were used as positive and negative controls, respectively. Samples were incubated for 3 and 24 h. Metabolically viable cells were evaluated by reduction of MTT, measured with spectrophotometric detection at 595.0 nm (Multiskan FC, Thermo Scientific) (Alarifi et al, 2013;Fernandes et al, 2010).…”

Antimicrobial Plant Extracts Encapsulated into Polymeric Beads for Potential Application on the Skin

“…HaCat cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 U/mL penicillin and 0.1 mg/mL streptomycin. Cell viability was evaluated using a 24 h-incubation protocol by the MTT assay, according to a procedure adapted from Fernandes et al [18]. Two independent experiments were performed, and four replicate cultures were used in each independent experiment.…”

Production and characterization of nanoparticles containing methanol extracts of Portuguese Lavenders

“…Cultures were then treated with the extracts (1-15 lg mL À1 ) for a 24 h-period. Afterward, the crystal violet assay was carried out as previously described [4]. Two independent experiments were performed and four replicate cultures were used in each independent experiment.…”