Protective role of nitric oxide during hydrogen peroxide-induced oxidative stress in tobacco plants

Dubovskaya, L. V.; Kolesneva, Ekaterina V; Knyazev, D. M.; Волотовский, И. Д.

doi:10.1134/s1021443707060064

Cited by 29 publications

(7 citation statements)

References 27 publications

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“…Its low molecular weight enables easy and rapid diffusion into the plant systems when compared to other NO donors. Intricate cross-talk and tradeoffs of NO have been found with other types of stress signals, especially H 2 O 2 (Dubovskaya et al, 2007 ; Sang et al, 2008a ; Tanou et al, 2009 , 2010 ), as mediated through strict transcriptional and translational control. This makes it clear that the identification of such transcriptional changes would aid elaboration of the mechanistic control of various functions.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

et al. 2016

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Imbalance between the accumulation and removal of nitric oxide and its derivatives is a challenge faced by all plants at the cellular level, and is especially important under stress conditions. Exposure of plants to various biotic and abiotic stresses causes rapid changes in cellular redox tone potentiated by the rise in reactive nitrogen species that serve as signaling molecules in mediating defensive responses. To understand mechanisms mediated by these signaling molecules, we performed a large-scale analysis of the Arabidopsis transcriptome induced by nitrosative stress. We generated an average of 84 and 91 million reads from three replicates each of control and 1 mM S-nitrosocysteine (CysNO)-infiltrated Arabidopsis leaf samples, respectively. After alignment, more than 95% of all reads successfully mapped to the reference and 32,535 genes and 55,682 transcripts were obtained. CysNO infiltration caused differential expression of 6436 genes (3448 up-regulated and 2988 down-regulated) and 6214 transcripts (3335 up-regulated and 2879 down-regulated) 6 h post-infiltration. These differentially expressed genes were found to be involved in key physiological processes, including plant defense against various biotic and abiotic stresses, hormone signaling, and other developmental processes. After quantile normalization of the FPKM values followed by student's T-test (P < 0.05) we identified 1165 DEGs (463 up-regulated and 702 down-regulated) with at least 2-folds change in expression after CysNO treatment. Expression patterns of selected genes involved in various biological pathways were verified using quantitative real-time PCR. This study provides comprehensive information about plant responses to nitrosative stress at transcript level and would prove helpful in understanding and incorporating mechanisms associated with nitrosative stress responses in plants.

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Section: Discussionmentioning

confidence: 99%

Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

et al. 2016

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“…In general, low concentrations of H 2 O 2 are reported to protect against oxidative and abiotic stress, by activating defence responses aimed at maintaining cellular redox homeostasis; concentrations in a narrow range of concentrations trigger PCD, while higher concentrations can cause necrosis (Van Breusegem and Dat, 2006;Laloi et al, 2006;de Pinto et al, 2006). In fact, previous work has shown that when H 2 O 2 is produced above certain levels, cell death is induced in a concentration-dependent manner (Houot et al, 2001;de Pinto et al, 2002;Dubovskaya et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H₂O₂treatment

Ortiz-Espín

Locato

Camejo

et al. 2015

Ann Bot

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A decreased content of endogenous H2O2 may be responsible in part for the delayed cell death found in over-expressing cells, in which changes in oxidative parameters and antioxidants were less extended after the oxidative treatment. It is concluded that PsTrxo1 transformation protects TBY-2 cells from exogenous H2O2, thus increasing their viability via a process in which not only antioxidants but also Trxo1 seem to be involved.

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“…N 2 O 3 or ONOO − in PQ treated Azolla might be the cause of reversal of protective effect of SNP at higher concentration (200 µM). Dubovskaya et al (2007) reported that the exogenous SNP and hydrogen peroxide exerted anatagonostic effects on tobacco leaves at micromolar concentration but induced synergistic effect at millimolar concentration, suggesting that NO performs a dual role in plants, acting as antioxidant (scavenger of reactive oxygen species) and as a signaling messenger.…”

Section: Ros Such As O −mentioning

confidence: 99%

Differential responses of hydrogen peroxide, lipid peroxidation and antioxidant enzymes in Azolla microphylla exposed to paraquat and nitric oxide

et al. 2012

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The present investigation was carried out to decipher the interplay between paraquat (PQ) and exogenously applied nitric oxide (NO) in Azolla microphylla. The addition of PQ (8 µM) increased the activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) by 1.7, 2.7, 3.9 and 1.9 folds respectively than that control in the fronds of Azolla. The amount of H2O2 was also enhanced by 2.7 times in the PQ treated plants than that of control. The supplementation of sodium nitroprusside (SNP) from 8-100 µM along with PQ, suppressed the activities of antioxidative enzymes and the amount of H2O2 compared to PQ alone. The drop in the activity of antioxidative enzymes -SOD, GPX, CAT and APX was highest (39.9%, 48.4%, 41.6% and 41.3% respectively) on the supplementation of 100 µM SNP with PQ treated fronds compared to PQ alone. The addition of NO scavengers along with NO donor in PQ treated fronds neutralized the effect of exogenously supplied NO. This indicates that NO can effectively protect Azolla against PQ toxicity by quenching reactive oxygen species. However, 200 µM of SNP reversed the protective effect of lower concentration of NO donor against herbicide toxicity. Our study clearly suggests that (i) SNP released NO can work both as cytoprotective and cytotoxic in concentration dependent manner and (ii) involvement of NO in protecting Azolla against PQ toxicity.

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Protective role of nitric oxide during hydrogen peroxide-induced oxidative stress in tobacco plants

Cited by 29 publications

References 27 publications

Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H₂O₂treatment

Differential responses of hydrogen peroxide, lipid peroxidation and antioxidant enzymes in Azolla microphylla exposed to paraquat and nitric oxide

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Protective role of nitric oxide during hydrogen peroxide-induced oxidative stress in tobacco plants

Cited by 29 publications

References 27 publications

Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana

Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H2O2treatment

Differential responses of hydrogen peroxide, lipid peroxidation and antioxidant enzymes in Azolla microphylla exposed to paraquat and nitric oxide

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Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H₂O₂treatment