Protein adsorption dissociation constants in various types of biochromatography

Patel, Drashti; Luo, Robert G.

doi:10.1016/s0167-2991(99)80573-4

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…In terms of the magnitude of the K values obtained in these experiments, there are, unfortunately, no directly comparable K values available in the literature. However, the order of magnitude of the K values obtained from these MALDI experiments is consistent with that of a large number of K values reported for other proteins on various surfaces . In addition, recent computational and experimental studies of the interaction of a small model peptide GGGKGGG, with the hydrophobic surface of a methyl-terminated self-assembled monolayer, yielded values of 1.1 × 10 4 and 1.1 × 10 5 L/mol, respectively. − …”

Section: Resultssupporting

confidence: 87%

“…The techniques used in the determination of equilibrium binding constants include chromatography, , radiotracer techniques, , electrophoresis, surface plasmon resonance spectroscopy, , colorimetric assays, , and fluorescence spectroscopy . Patel and Luo have summarized the equilibrium binding data obtained from biochromatography and developed a chart of relative protein adsorption strength …”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Surface−Protein Binding Constants by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Zhang

Kinsel

2003

Langmuir

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Evaluation of Surface−Protein Binding Constants by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Zhang

Kinsel

2003

Langmuir

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“…Crucial for specific affinity ligands is the consideration of their dissociation constants. For recombinant proteins, dissociation constants of 10 -4 -10 -10 M are usually considered a good working range [115]. Optimal affinity ligands allow fast binding kinetics for high flow rates but mild elution conditions to avoid reduction in immunogenicity or activity.…”

Section: Surface Chemistry Of the Stationary Phase And The Composition mentioning

confidence: 99%

Downstream processing of cell culture-derived virus particles

Wolf

Reichl

2011

Expert Review of Vaccines

121

102

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Manufacturing of cell culture-derived virus particles for vaccination and gene therapy is a rapidly growing field in the biopharmaceutical industry. The process involves a number of complex tasks and unit operations ranging from selection of host cells and virus strains for the cultivation in bioreactors to the purification and formulation of the final product. For the majority of cell culture-derived products, efforts focused on maximization of bioreactor yields, whereas design and optimization of downstream processes were often neglected. Owing to this biased focus, downstream procedures today often constitute a bottleneck in various manufacturing processes and account for the majority of the overall production costs. For efficient production methods, particularly in sight of constantly increasing economic pressure within human healthcare systems, highly productive downstream schemes have to be developed. Here, we discuss unit operations and downstream trains to purify virus particles for use as vaccines and vectors for gene therapy.

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Chemical and physical properties of regenerative medicine materials controlling stem cell fate

et al. 2011

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Regenerative medicine is a multidisciplinary field utilizing the potential of stem cells and the regenerative capability of the body to restore, maintain, or enhance tissue and organ functions. Stem cells are unspecialized cells that can self-renew but also differentiate into several somatic cells when subjected the appropriate environmental cues. The ability to reliably direct stem cell fate would provide tremendous potential for basic research and clinical therapies. Proper tissue function and regeneration rely on the spatial and temporal control of biophysical and biochemical cues, including soluble molecules, cell-cell contacts, cell-extracellular matrix contacts, and physical forces. The mechanisms involved remain poorly understood. This review focuses on the stem cell-extracellular matrix interactions by summarizing the observations of the effects of material variables (such as overall architecture, surface topography, charge, ζ-potential, surface energy, and elastic modulus) on the stem cell fate. It also deals with the mechanisms underlying the effects of these extrinsic, material variables. Insight in the environmental interactions of the stem cells is crucial for the development of new material-based approaches for cell culture experiments and future experimental and clinical regenerative medicine applications.

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Protein adsorption dissociation constants in various types of biochromatography

Cited by 4 publications

References 33 publications

Evaluation of Surface−Protein Binding Constants by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Evaluation of Surface−Protein Binding Constants by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Downstream processing of cell culture-derived virus particles

Chemical and physical properties of regenerative medicine materials controlling stem cell fate

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