Protein Adsorption into Mesopores: A Combination of Electrostatic Interaction, Counterion Release, and van der Waals Forces

Moerz, Sebastian T.; Huber, Patrick

doi:10.1021/la404947j

Cited by 67 publications

(62 citation statements)

References 46 publications

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“…Similar values of the ES potential were observed when using liposomes as a template to build PE nanocapsules [146]. Note also the properties of electrostatically driven adsorption of proteins onto highly charged silica surfaces and inside porous substrates, see [147][148][149]. At all these conditions, the nonlinear ES effects can come into play.…”

Section: Discussionsupporting

confidence: 63%

Critical adsorption of polyelectrolytes onto planar and convex highly charged surfaces: the nonlinear Poisson–Boltzmann approach

2016

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We study the adsorption-desorption transition of polyelectrolyte chains onto planar, cylindrical and spherical surfaces with arbitrarily high surface charge densities by massive Monte Carlo computer simulations. We examine in detail how the well known scaling relations for the threshold transitiondemarcating the adsorbed and desorbed domains of a polyelectrolyte near weakly charged surfacesare altered for highly charged interfaces. In virtue of high surface potentials and large surface charge densities, the Debye-Hückel approximation is often not feasible and the nonlinear Poisson-Boltzmann approach should be implemented. At low salt conditions, for instance, the electrostatic potential from the nonlinear Poisson-Boltzmann equation is smaller than the Debye-Hückel result, such that the required critical surface charge density for polyelectrolyte adsorption s c increases. The nonlinear relation between the surface charge density and electrostatic potential leads to a sharply increasing critical surface charge density with growing ionic strength, imposing an additional limit to the critical salt concentration above which no polyelectrolyte adsorption occurs at all. We contrast our simulations findings with the known scaling results for weak critical polyelectrolyte adsorption onto oppositely charged surfaces for the three standard geometries. Finally, we discuss some applications of our results for some physical-chemical and biophysical systems.

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Section: Discussionsupporting

confidence: 63%

Critical adsorption of polyelectrolytes onto planar and convex highly charged surfaces: the nonlinear Poisson–Boltzmann approach

2016

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“…A PDI less than 0.25 suggested that the suspension had a narrow distribution (Ibrahim et al 2014;Ninjbadgar et al 2015). During the milling process, nanoparticle agglomeration was mainly caused by van der Waals forces and electrostatic interactions (Rance et al 2010;Moerz and Huber 2015). For anionic surfactant, SDS could provide sufficient electric charge to induce electrostatic repulsion for the suspended particles (Teeranachaideekul et al 2008).…”

Section: Preparation Of the Azoxystrobin Nanosuspensionmentioning

confidence: 99%

Preparation, characterization, and evaluation of azoxystrobin nanosuspension produced by wet media milling

et al. 2018

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To improve the bioavailability of the poorly water-soluble fungicide, an azoxystrobin nanosuspension was prepared by the wet media milling method. Due to their reduced mean particle size and polydispersity index, 1-Dodecanesulfonic acid sodium salt and polyvinylpyrrolidone K30 were selected from six conventional surfactants, the content only accounting for 15% of the active compound. The mean particle size, polydispersity index, and potential of the nanosuspension were determined to be 238.1 ± 1.5 nm, 0.17 ± 0.02 and − 31.8 ± 0.3 mV, respectively. The lyophilized nanosuspension mainly retained crystalline state, with only a little amorphous content as determined by powder X-ray diffraction. Compared to conventional fungicide formulations, the nanosuspension presented an increased retention volume and a reduced contact angle, indicating enhanced wettability and adhesion. In addition, the azoxystrobin nanosuspension showed the highest antifungal activity, with a medial lethal concentration of 1.4243 μg/mL against Fusarium oxysporum. In optical micrographs, hyphal deformations of thinner and intertwined hyphae were detected in the exposed group. Compared to the control group, the total soluble protein content, superoxide dismutase, and catalase activities were initially increased and then decreased with prolonged exposure time. The azoxystrobin nanosuspension reduced the defensive antioxidant capability of Fusarium oxysporum and resulted in the generation of excessive reactive oxygen species. This study provides a novel method for preparing nanosuspension formulation of poorly soluble antifungal agents to enhance the biological activity and decrease the negative environmental impact.

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“…It has also been used in the adsorption/immobilization of carbonic anhydrase [23] and biomolecules such as bovine serum albumin [24][25][26], lysozyme [27][28][29], myoglobin [30], lipase [31][32][33], cellulose [34], trypsin [26,35], aminoacids [36], laccase [37], glucose oxidase [38], cytochrome c and xylanase [39,40].…”

Section: A N U S C R I P Tmentioning

confidence: 99%

The effect of structure modifying agents in the SBA-15 for its application in the biomolecules adsorption

Santos

Cecilia

Vilarrasa-García

et al. 2016

Microporous and Mesoporous Materials

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Protein Adsorption into Mesopores: A Combination of Electrostatic Interaction, Counterion Release, and van der Waals Forces

Cited by 67 publications

References 46 publications

Critical adsorption of polyelectrolytes onto planar and convex highly charged surfaces: the nonlinear Poisson–Boltzmann approach

Critical adsorption of polyelectrolytes onto planar and convex highly charged surfaces: the nonlinear Poisson–Boltzmann approach

Preparation, characterization, and evaluation of azoxystrobin nanosuspension produced by wet media milling

The effect of structure modifying agents in the SBA-15 for its application in the biomolecules adsorption

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